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mTeSR™3 d

用于人胚胎干细胞和iPS细胞悬浮培养的无血清培养基
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产品号 #03950_C

用于人胚胎干细胞和iPS细胞悬浮培养的无血清培养基

产品组分包括

  • mTeSR™3D Seed基础培养基，400 mL
  • mTeSR™3D Seed 5X 补充剂，100 mL
  • mTeSR™3D Feed 补充剂 A，100 mL
  • mTeSR™3D Feed 补充剂 B，12 mL
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  1. Gentle Cell Dissociation Reagent
    Gentle Cell Dissociation Reagent

    cGMP, enzyme-free cell dissociation reagent

  2. 37μm Reversible Strainers|27215,27250
    Reversible Strainers

    Reversible strainer for filtration of single cells and isolation of cellular aggregates, including embryoid bodies and spheroids

  3. Y-27632 (Dihydrochloride)
    Y-27632 (Dihydrochloride)

    RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2

总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

mTeSR™3D用于未分化的人胚胎干细胞（ES）和人诱导多能干细胞（iPS）细胞在3D悬浮培养中的扩增和放大。新颖的进料批工作流程节省了时间和媒体。每日饲料补充营养，并消除非通过日媒介交换的需要。

mTeSR™3D是一种专门的无血清制剂。该培养系统与一系列悬浮培养容器兼容。

Subtype
Specialized Media
 
Cell Type
Pluripotent Stem Cells
 
Species
Human
 
Application
Cell Culture, Expansion
 
Brand
TeSR
 
Area of Interest
Stem Cell Biology
 
Formulation Category
Serum-Free
 

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实验数据

Figure 1. hPSC growth in mTeSR™3D

mTeSR™3D supports expansion of hES and hiPS cells over multiple passages in aggregate suspension culture. Shown are cumulative viable cell numbers for (A) hESC line H9 cultured in shaker bottles (10 passages) and (B) hiPSC line STiPS-F016 cultured in spinner flasks (5 passages). Some cell lines may experience 1 passage of adaptation from 2D to 3D, as shown in (B).

Figure 2. hPSC aggregate morphology in mTeSR3D

Representative images of hPSC aggregates cultured in mTeSR™3D. (A) H9 cells (B) H7 cells (C) STiPS-F016 cells.


Figure 3. Expression of undifferentiated cell markers

hPSCs cultured in mTeSR™3D maintain high expression of OCT4. (A) H9 cells after 10 passages in shaker bottles. (B) STiPS-F016 cells after 5 passages in spinner flasks. Representative flow cytometry plots shown.

Figure 4. Tri-lineage Differentiation

hPSCs cultured in mTeSR™3D can be efficiently differentiated to all three germ layers. Trilineage differentiation potential was assessed using the STEMdiff™ Trilineage Differentiation Kit (Cat # 05230).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
mTeSR™3D
Catalog #
03950
Lot #
All
Language
English
Document Type
Technical Manual
Product Name
mTeSR™3D
Catalog #
03950
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
mTeSR™3D
Catalog #
03950
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
mTeSR™3D
Catalog #
03950
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
mTeSR™3D
Catalog #
03950
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
mTeSR™3D
Catalog #
03950
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

更多信息

更多信息
种属 Human
配方类别 Serum-Free
法律声明:

mTeSR™3D is manufactured and sold under global exclusive license from Accellta for culture medium for hPSCs in suspension under feeder-free, non-adherent conditions. 本产品系基于WiCell™研究院（WiCell™ Research Institute）知识产权授权许可开发。本产品的出售目的仅限于基于不可转让、用途受限之许可下的研究用途（无论购买者为学术机构或营利性主体）。购买本产品并不被授予为商业用途（即，以获利为目的的任何行为，如将本产品用于制造用途、或转售本产品或使用本产品所制成的任何材料、或将本产品或使用本产品所制成的材料用于提供服务）或临床应用（即，将本产品或本产品所用材料应用于人体）而出售、使用或另行转让本产品的权利，或出于基础临床前研究应用（包括但不限于畸胎瘤试验）以外的目的，由营利性主体或与其合作，将使用本产品制造的任何材料植入动物体内的权利。
购买者若不同意前述条款和条件，应保持产品完好，将其退还出售方以获退款。

质量保证:

产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
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