产品号 #03950_C
用于人胚胎干细胞和iPS细胞悬浮培养的无血清培养基
若您需要咨询产品或有任何技术问题,请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系
cGMP, enzyme-free cell dissociation reagent
Reversible strainer for filtration of single cells and isolation of cellular aggregates, including embryoid bodies and spheroids
RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2
Compatible antibodies for purity assessment of isolated cells
mTeSR™3D用于未分化的人胚胎干细胞(ES)和人诱导多能干细胞(iPS)细胞在3D悬浮培养中的扩增和放大。新颖的进料批工作流程节省了时间和媒体。每日饲料补充营养,并消除非通过日媒介交换的需要。
mTeSR™3D是一种专门的无血清制剂。该培养系统与一系列悬浮培养容器兼容。
Subtype
Specialized Media
Cell Type
Pluripotent Stem Cells
Species
Human
Application
Cell Culture, Expansion
Brand
TeSR
Area of Interest
Stem Cell Biology
Formulation Category
Serum-Free
Figure 1. hPSC growth in mTeSR™3D
mTeSR™3D supports expansion of hES and hiPS cells over multiple passages in aggregate suspension culture. Shown are cumulative viable cell numbers for (A) hESC line H9 cultured in shaker bottles (10 passages) and (B) hiPSC line STiPS-F016 cultured in spinner flasks (5 passages). Some cell lines may experience 1 passage of adaptation from 2D to 3D, as shown in (B).
Figure 2. hPSC aggregate morphology in mTeSR3D
Representative images of hPSC aggregates cultured in mTeSR™3D. (A) H9 cells (B) H7 cells (C) STiPS-F016 cells.
Figure 3. Expression of undifferentiated cell markers
hPSCs cultured in mTeSR™3D maintain high expression of OCT4. (A) H9 cells after 10 passages in shaker bottles. (B) STiPS-F016 cells after 5 passages in spinner flasks. Representative flow cytometry plots shown.
Figure 4. Tri-lineage Differentiation
hPSCs cultured in mTeSR™3D can be efficiently differentiated to all three germ layers. Trilineage differentiation potential was assessed using the STEMdiff™ Trilineage Differentiation Kit (Cat # 05230).
请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。
本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。
Thank you for your interest in IntestiCult™ Organoid Growth Medium (Human). Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
种属 | Human |
---|---|
配方类别 | Serum-Free |
功能检测试剂盒,用于评估人ES和iPS细胞定向分化至三个胚层的多能性
防止细胞粘附的培养容器的冲洗液
小鼠单克隆IgG2b抗体,抗人OCT4(OCT3)
cGMP标准、无饲养层的人胚胎干细胞(ES)和诱导多能干细胞(iPS)维持培养基
扫描二维码或搜索微信号STEMCELLTech,即可关注我们的微信平台,第一时间接收丰富的技术资源和最新的活动信息。
如您有任何问题,欢迎发消息给STEMCELLTech微信公众平台,或与我们通过电话/邮件联系:400 885 9050 INFO.CN@STEMCELL.COM。