PneumaCult™气道类器官试剂盒

无血清和无bpe培养基用于气道类器官的高效建立和分化

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产品号 #（选择产品）

产品号 #05060_C

无血清和无bpe培养基用于气道类器官的高效建立和分化

产品优势

三维体外系统，概括了人体内气道的关键特征
用于人气道上皮细胞扩增和分化的完整培养基配方，与PneumaCult™-Ex Plus兼容
方便的格式和易于使用的协议，提供一致的结果

产品组分包括

PneumaCult™气道类器官基础培养基，450 mL（目录#05061）
PneumaCult™气道类器官播种补充剂，10 mL（目录#05062）
PneumaCult™气道类器官分化补充剂，40ml（目录#05063）

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要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。

PneumaCult™-Ex Plus Medium
Serum- and BPE-free medium for expansion of primary human airway epithelial cells
Animal Component-Free Cell Dissociation Kit
Dissociation kit for human stem and progenitor cells
Hydrocortisone Stock Solution
Cell culture supplement
Heparin Solution
Cell culture supplement
Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells

总览

实验数据

产品说明书及文档

应用领域

总览

PneumaCult™气道类器官试剂盒是一种不含血清和bpe的培养基系统，用于有效地建立和分化源自人支气管上皮细胞（HBECs）的气道类器官。
在PneumaCult™-Ex Plus培养基（#05040）中进行2D扩展后，PneumaCult™气道类器官种子培养基允许开始3D类器官培养，然后使用PneumaCult™气道类器官分化培养基获得形态学相关的气道类器官。气道类器官为研究气道上皮提供了一种功能性的体外器官型培养系统。当完全分化时，类器官表现为中央管腔，周围是极化的气道上皮细胞层，由分化的细胞类型组成，如纤毛细胞和杯状细胞。气道类器官培养的应用包括肺部疾病建模、药物疗效和毒性筛选以及气道上皮功能研究。

浏览我们的常见问题（faq）关于使用PneumaCult™培养气道类器官的问题。

如果您打算将此产品用于商业用途，请通过www.huborganoids.nl获取商业用途许可证或HUB许可的相关说明。

Subtype
Specialized Media

Cell Type
Airway Cells

Species
Human

Application
Cell Culture, Differentiation, Expansion, Organoid Culture

Brand
PneumaCult

Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Organoids, Respiratory Research

Formulation Category
Serum-Free

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实验数据

Schematic of human airway organoid culture workflow using PneumaCult™ Airway Organoid Kit

Figure 1. Overview of the PneumaCult™ Culture System for Human Airway Organoid Generation

In the early two-dimensional expansion phase of the human airway organoid culture procedure, HBECs are expanded using PneumaCult™-Ex Plus Medium. The HBECs are then embedded into a Matrigel® dome and expanded for 4 - 7 days using PneumaCult™ Airway Organoid Seeding Medium. Following the expansion, the HBECs are differentiated using PneumaCult™ Airway Organoid Differentiation Medium for an additional 21+ days.

Fully differentiated human airway organoids generated using PneumaCult™ Airway Organoid Kit display physiologically relevant morphology.

Figure 2. Fully Differentiated Human Airway Organoids Generated Using PneumaCult™ Airway Organoid Kit

(A) Bright-field image of airway organoids growing in PneumaCult™ Airway Organoid Seeding Medium at day 7 exhibit basal cell spheroid morphology. (B) Bright-field image of airway organoids differentiated in PneumaCult™ Airway Organoid Differentiation Medium at day 21 exhibit hollow lumens. (C) Airway organoid stained for ZO-1 (junction protein marker, red), MUC5AC (goblet cell marker, purple), AC-Tubulin (ciliated cell marker, green), and DAPI (nuclei, blue).

Comparison of Forskolin-treated airway organoids derived from healthy donors and CF donors.

Figure 3. Forskolin-Induced Swelling of Airway Organoids

(A) Forskolin-treated organoids derived from healthy donors increased in size compared to the DMSO control, indicating functional CFTR protein expression. (B) Forskolin-induced swelling is lost in organoids derived from CF donors, but re-established in VX-809-treated airway organoids. Error bars represent ± 95% confidence interval for the mean (n=3). Bright-field images of airway organoids taken during the Forskolin swelling assay at (C) 0 hours and (D) 6 hours show organoid swelling after treatment.

Ciliated cell percentage in fully differentiated airway organoids derived from healthy and cystic fibrosis (CF) donors using PneumaCult™ Airway Organoid Kit at different passages.

Figure 4. Fully Differentiated Airway Organoids Retain Morphological Characteristics at Different Passages

The ciliated cell percentage in organoids grown from (A) healthy and (B) CF donors using PneumaCult™ Airway Organoid Kit increased from P3 to P5. The total and ciliated cells were counted using a hemocytometer. Error bars represent ± 95% confidence interval for the mean (n=3).