若您需要咨询产品或有任何技术问题,请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系

TeSR™-E5

成分明确、无血清、无异种成分的多能干细胞培养基

产品号 #(选择产品)

产品号 #05916_C

成分明确、无血清、无异种成分的多能干细胞培养基

产品组分包括

  • TeSR™-E5/E6 基础培养基,475 mL
  • TeSR™-E5 20X 补充剂,25 mL

总览

TeSR™-E5 是一种成分明确、无血清且无异种成分的培养基,基于 TeSR™-E8™ 的配方,但不含转化生长因子 β(TGF-β)、碱性成纤维细胞生长因子(bFGF)和胰岛素。它可用作人胚胎干(ES)细胞和诱导多能干(iPS)细胞分化的基础培养基,或用于其他需要去除上述细胞因子和胰岛素的应用场景。

亚型
专用培养基
 
细胞类型
多能干细胞
 
种属

 
应用
细胞培养,鉴定,分化
 
品牌
TeSR
 
研究领域
药物发现和毒理检测,干细胞生物学
 
制剂类别
无血清,Xeno-Free
 

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Product Name
TeSR™-E5
Catalog #
05916
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
TeSR™-E5
Catalog #
05916
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
TeSR™-E5
Catalog #
05916
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。

相关材料与文献

技术资料 (5)

文献 (1)

Increased culture density is linked to decelerated proliferation, prolonged G1 phase, and enhanced propensity for differentiation of self-renewing human pluripotent stem cells. Wu J et al. Stem cells and development 2015 APR

Abstract

Human pluripotent stem cells (hPSCs) display a very short G1 phase and rapid proliferation kinetics. Regulation of the cell cycle, which is linked to pluripotency and differentiation, is dependent on the stem cell environment, particularly on culture density. This link has been so far empirical and central to disparities in the growth rates and fractions of self-renewing hPSCs residing in different cycle phases. In this study, hPSC cycle progression in conjunction with proliferation and differentiation were comprehensively investigated for different culture densities. Cell proliferation decelerated significantly at densities beyond 50×10(4) cells/cm(2). Correspondingly, the G1 fraction increased from 25% up to 60% at densities greater than 40×10(4) cells/cm(2) while still hPSC pluripotency marker expression was maintained. In parallel, expression of the cycle inhibitor CDKN1A (p21) was increased, while that of p27 and p53 did not change significantly. After 4 days of culture in an unconditioned medium, greater heterogeneity was noted in the differentiation outcomes and was limited by reducing the density variation. A quantitative model was constructed for self-renewing and differentiating hPSC ensembles to gain a better understanding of the link between culture density, cycle progression, and stem cell state. Results for multiple hPSC lines and medium types corroborated experimental findings. Media commonly used for maintenance of self-renewing hPSCs exhibited the slowest kinetics of induction of differentiation (kdiff), while BMP4 supplementation led to 14-fold higher kdiff values. Spontaneous differentiation in a growth factor-free medium exhibited the largest variation in outcomes at different densities. In conjunction with the quantitative framework, our findings will facilitate rationalizing the selection of cultivation conditions for the generation of stem cell therapeutics.

更多信息

更多信息
种属 Human
配方类别 Serum-Free, Xeno-Free
法律声明:

本产品系基于WiCell™研究院(WiCell™ Research Institute)知识产权授权许可开发。本产品的出售目的仅限于基于不可转让、用途受限之许可下的研究用途(无论购买者为学术机构或营利性主体)。购买本产品并不被授予为商业用途(即,以获利为目的的任何行为,如将本产品用于制造用途、或转售本产品或使用本产品所制成的任何材料、或将本产品或使用本产品所制成的材料用于提供服务)或临床应用(即,将本产品或本产品所用材料应用于人体)而出售、使用或另行转让本产品的权利,或出于基础临床前研究应用(包括但不限于畸胎瘤试验)以外的目的,由营利性主体或与其合作,将使用本产品制造的任何材料植入动物体内的权利。
购买者若不同意前述条款和条件,应保持产品完好,将其退还出售方以获退款。

质量保证:

产品仅供研究使用,不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息,请访问 STEMCELL.CN/COMPLIANCE.
Copyright © 2025 by STEMCELL Technologies. All rights reserved.