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产品号 #05990_C
无饲养层、无动物成分的人胚胎干细胞和诱导多能干细胞维持培养基
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总览
TeSR™-E8™ 是一款无饲养层、无动物成分的培养基，适用于培养人胚胎干细胞 (ES) 和人诱导性多能干细胞 (iPS)。它基于威斯康星大学麦迪逊分校 James Thomson 博士实验室研发的 E8 配方，该实验室是 mTeSR™1 研发的主导研发团队，mTeSR™1 是目前发表最广泛的多能干细胞无饲养层培养基。
与 TeSR™ 产品家族中的其他产品一样，TeSR™-E8™ 培养基以最高标准和严谨工艺制造。该培养基专门研发，仅包含维持 ES 和 iPS 细胞生长所需的必需成分，是多能干细胞培养中最简化的培养基。TeSR™-E8™ 可搭配 Corning® Matrigel® hESC-Qualified Matrix（Corning 354277）一起使用，若需完全明确的无异种成分系统，可选用 Vitronectin XF™（目录号 07180）或 Laminin-521（目录号 77003）作为培养基质。
与 TeSR™ 产品家族中的其他产品一样，TeSR™-E8™ 培养基以最高标准和严谨工艺制造。该培养基专门研发，仅包含维持 ES 和 iPS 细胞生长所需的必需成分，是多能干细胞培养中最简化的培养基。TeSR™-E8™ 可搭配 Corning® Matrigel® hESC-Qualified Matrix（Corning 354277）一起使用，若需完全明确的无异种成分系统，可选用 Vitronectin XF™（目录号 07180）或 Laminin-521（目录号 77003）作为培养基质。
与 TeSR™ 产品家族中的其他产品一样，TeSR™-E8™ 培养基以最高标准和严谨工艺制造。该培养基专门研发，仅包含维持 ES 和 iPS 细胞生长所需的必需成分，是多能干细胞培养中最简化的培养基。TeSR™-E8™ 可搭配 Corning® Matrigel® hESC-Qualified Matrix（Corning 354277）一起使用，若需完全明确的无异种成分系统，可选用 Vitronectin XF™（目录号 07180）或 Laminin-521（目录号 77003）作为培养基质。
与 TeSR™ 产品家族中的其他产品一样，TeSR™-E8™ 培养基以最高标准和严谨工艺制造。该培养基专门研发，仅包含维持 ES 和 iPS 细胞生长所需的必需成分，是多能干细胞培养中最简化的培养基。TeSR™-E8™ 可搭配 Corning® Matrigel® hESC-Qualified Matrix（Corning 354277）一起使用，若需完全明确的无异种成分系统，可选用 Vitronectin XF™（目录号 07180）或 Laminin-521（目录号 77003）作为培养基质。
亚型
专用培养基
细胞类型
多能干细胞
种属
人
应用
细胞培养，扩增，培养
品牌
TeSR
研究领域
干细胞生物学
制剂类别
Animal Component-Free
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (18)
文献 (4)
A Novel Toolkit for Characterizing the Mechanical and Electrical Properties of Engineered Neural Tissues. Biosensors 2019 apr
Abstract
We have designed and validated a set of robust and non-toxic protocols for directly evaluating the properties of engineered neural tissue. These protocols characterize the mechanical properties of engineered neural tissues and measure their electrophysical activity. The protocols obtain elastic moduli of very soft fibrin hydrogel scaffolds and voltage readings from motor neuron cultures. Neurons require soft substrates to differentiate and mature, however measuring the elastic moduli of soft substrates remains difficult to accurately measure using standard protocols such as atomic force microscopy or shear rheology. Here we validate a direct method for acquiring elastic modulus of fibrin using a modified Hertz model for thin films. In this method, spherical indenters are positioned on top of the fibrin samples, generating an indentation depth that is then correlated with elastic modulus. Neurons function by transmitting electrical signals to one another and being able to assess the development of electrical signaling serves is an important verification step when engineering neural tissues. We then validated a protocol wherein the electrical activity of motor neural cultures is measured directly by a voltage sensitive dye and a microplate reader without causing damage to the cells. These protocols provide a non-destructive method for characterizing the mechanical and electrical properties of living spinal cord tissues using novel biosensing methods.
Comprehensive Cell Surface Protein Profiling Identifies Specific Markers of Human Naive and Primed Pluripotent States. Cell stem cell 2017 MAR
Abstract
Human pluripotent stem cells (PSCs) exist in naive and primed states and provide important models to investigate the earliest stages of human development. Naive cells can be obtained through primed-to-naive resetting, but there are no reliable methods to prospectively isolate unmodified naive cells during this process. Here we report comprehensive profiling of cell surface proteins by flow cytometry in naive and primed human PSCs. Several naive-specific, but not primed-specific, proteins were also expressed by pluripotent cells in the human preimplantation embryo. The upregulation of naive-specific cell surface proteins during primed-to-naive resetting enabled the isolation and characterization of live naive cells and intermediate cell populations. This analysis revealed distinct transcriptional and X chromosome inactivation changes associated with the early and late stages of naive cell formation. Thus, identification of state-specific proteins provides a robust set of molecular markers to define the human PSC state and allows new insights into the molecular events leading to naive cell resetting.
Peripheral blood derived induced pluripotent stem cells (iPSCs) from a female with familial hypertrophic cardiomyopathy. Stem cell research 2017
Abstract
Induced pluripotent stem cells (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) obtained from a 62-year-old female with familial hypertrophic cardiomyopathy (HCM). PBMCs were reprogrammed to a pluripotent state following transfection with non-integrative episomal vectors carrying reprogramming factors OCT4, SOX2, LIN28, KLF4 and L-MYC. iPSCs were shown to express pluripotency markers, possess trilineage differentiation potential, carry rare variants identified in DNA isolated directly from the patient's whole blood, have a normal karyotype and no longer carry episomal vectors for reprogramming. This line is a useful resource for identifying unknown genetic causes of HCM.
更多信息
| 种属 | Human |
|---|---|
| 配方类别 | Animal Component-Free |
相关产品
法律声明:
本产品系基于WiCell™研究院（WiCell™ Research Institute）知识产权授权许可开发。本产品的出售目的仅限于基于不可转让、用途受限之许可下的研究用途（无论购买者为学术机构或营利性主体）。购买本产品并不被授予为商业用途（即，以获利为目的的任何行为，如将本产品用于制造用途、或转售本产品或使用本产品所制成的任何材料、或将本产品或使用本产品所制成的材料用于提供服务）或临床应用（即，将本产品或本产品所用材料应用于人体）而出售、使用或另行转让本产品的权利，或出于基础临床前研究应用（包括但不限于畸胎瘤试验）以外的目的，由营利性主体或与其合作，将使用本产品制造的任何材料植入动物体内的权利。
购买者若不同意前述条款和条件，应保持产品完好，将其退还出售方以获退款。
质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
本产品系基于WiCell™研究院（WiCell™ Research Institute）知识产权授权许可开发。本产品的出售目的仅限于基于不可转让、用途受限之许可下的研究用途（无论购买者为学术机构或营利性主体）。购买本产品并不被授予为商业用途（即，以获利为目的的任何行为，如将本产品用于制造用途、或转售本产品或使用本产品所制成的任何材料、或将本产品或使用本产品所制成的材料用于提供服务）或临床应用（即，将本产品或本产品所用材料应用于人体）而出售、使用或另行转让本产品的权利，或出于基础临床前研究应用（包括但不限于畸胎瘤试验）以外的目的，由营利性主体或与其合作，将使用本产品制造的任何材料植入动物体内的权利。
购买者若不同意前述条款和条件，应保持产品完好，将其退还出售方以获退款。
质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
