技术资料

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核酸的分离、扩增和检测

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STEMscript
产品手册
Highway1™: Fast Cell Sorting from Bone Marrow

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仪器及软件

品牌：

Highway1
产品手册
Highway1™: High-Speed Enrichment from Blood Cells

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仪器及软件

品牌：

Highway1
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Highway1™: Automated Cell Sorting of PBMCs

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仪器及软件

品牌：

Highway1
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Highway1™: High-Purity Sorting of Bacterially-Infected Cells

产品类型：

仪器及软件

品牌：

Highway1
产品手册
Highway1™: Sorting Cell Subtypes from Murine Melanoma

产品类型：

仪器及软件

品牌：

Highway1
产品手册
Highway1™: Truly Gentle Enrichment Sorting

产品类型：

仪器及软件

品牌：

Highway1
Y. Zhang et al. ( 2015) The Journal of Immunology 194 5937-5947

Genetic Vaccines To Potentiate the Effective CD103+ Dendritic Cell-Mediated Cross-Priming of Antitumor Immunity

The development of effective cancer vaccines remains an urgent,but as yet unmet,clinical need. This deficiency is in part due to an incomplete understanding of how to best invoke dendritic cells (DC) that are crucial for the induction of tumor-specific CD8(+) T cells capable of mediating durable protective immunity. In this regard,elevated expression of the transcription factor X box-binding protein 1 (XBP1) in DC appears to play a decisive role in promoting the ability of DC to cross-present Ags to CD8(+) T cells in the therapeutic setting. Delivery of DNA vaccines encoding XBP1 and tumor Ag to skin DC resulted in increased IFN-? production by plasmacytoid DC (pDC) from skin/tumor draining lymph nodes and the cross-priming of Ag-specific CD8(+) T cell responses associated with therapeutic benefit. Antitumor protection was dependent on cross-presenting Batf3(+) DC,pDC,and CD8(+) T cells. CD103(+) DC from the skin/tumor draining lymph nodes of the immunized mice appeared responsible for activation of Ag-specific naive CD8(+) T cells,but were dependent on pDC for optimal effectiveness. Similarly,human XBP1 improved the capacity of human blood- and skin-derived DC to activate human T cells. These data support an important intrinsic role for XBP1 in DC for effective cross-priming and orchestration of Batf3(+) DC-pDC interactions,thereby enabling effective vaccine induction of protective antitumor immunity. View Publication

产品号#：

17858

17858RF

100-0694

产品名：

EasySep™人CD14正选试剂盒II

RoboSep™ 人CD14正选试剂盒II

EasySep™人CD14正选试剂盒II
F. Cadamuro et al. (Feb 2023) Carbohydrate polymers 302 120395

3D bioprinted colorectal cancer models based on hyaluronic acid and signalling glycans.

In cancer microenvironment,aberrant glycosylation events of ECM proteins and cell surface receptors occur. We developed a protocol to generate 3D bioprinted models of colorectal cancer (CRC) crosslinking hyaluronic acid and gelatin functionalized with three signalling glycans characterized in CRC,3'-Sialylgalactose,6'-Sialylgalactose and 2'-Fucosylgalactose. The crosslinking,performed exploiting azide functionalized gelatin and hyaluronic acid and 4arm-PEG-dibenzocyclooctyne,resulted in biocompatible hydrogels that were 3D bioprinted with commercial CRC cells HT-29 and patient derived CRC tumoroids. The glycosylated hydrogels showed good 3D printability,biocompatibility and stability over the time. SEM and synchrotron radiation SAXS/WAXS analysis revealed the influence of glycosylation in the construct morphology,whereas MALDI-MS imaging showed that protein profiles of tumoroid cells vary with glycosylation,indicating that sialylation and fucosylation of ECM proteins induce diverse alterations to the proteome of the tumoroid and surrounding cells. View Publication

产品号#：

06010

产品名：

IntestiCult™ 类器官生长培养基 (人)
实验方案
How to Identify ALDH-Expressing Cells in Human Breast Tissue

研究方向：

上皮细胞生物学，癌症研究
技术公告
使用StemSpan™白血病细胞培养试剂盒进行体外药物筛选

细胞类型：

癌细胞及细胞系，白血病/淋巴瘤细胞，造血干/祖细胞
45:17

线上讲座
Troubleshooting In Vitro Expansion of Leukemic Cells

发布日期: 07/16/2019