Scientific Resources

科学海报
Fast and Easy Isolation of CD27-Positive Human Memory B Cells Using EasySep™ Releasable RapidSpheres™

产品类型：

Cell Isolation Products

Conference：

AAI 2017
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Human Primary Cells: It All Starts with the Right Cells

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Primary and Cultured Cells

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MegaCult，EasySep，MesenCult，MyeloCult，StemSpan
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Tools For Your Immunology Research

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EasySep，ImmunoCult，RoboSep，RosetteSep，SepMate
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15-Minute Mouse Cell Isolation

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Cell Isolation Products

品牌：

EasySep，RoboSep
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EasySep™ Direct: Cell Isolation from Whole Blood in 20 Minutes

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Cell Isolation Products

品牌：

EasySep
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ELISA Kits for Immunology Research

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ELISAs

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ELISA
科学海报
Fast and Easy Immunomagnetic Positive Selection of PE- or Biotin-Conjugated Antibody Labeled Cells with Releasable RapidSpheres™ Results in Functional, Pure and Particle-Free Cells

产品类型：

Cell Isolation Products

Conference：

CYTO 2016
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Cell Enrichment Prior to Cell Sorting

产品类型：

Cell Isolation Products

品牌：

EasySep，RosetteSep
1:46

视频
How to Isolate PBMCs from Whole Blood Using the SepMate™ PBMC Isolation Tubes: 15-Minute Protocol

发布日期: 3/23/18
技术窍门
Fully Automated, High-Purity Cell Isolation for Chimerism Labs

产品类型：

Cell Isolation Products

细胞类型：

B 细胞，T 细胞，淋巴细胞，髓系细胞
技术窍门
Enhance Sensitivity of Multiple Myeloma Testing with Purified CD138+ Plasma Cells

产品类型：

Cell Isolation Products

细胞类型：

B 细胞
文献

Ng PP et al. (OCT 2006) Blood 108 8 2745--54

Molecular events contributing to cell death in malignant human hematopoietic cells elicited by an IgG3-avidin fusion protein targeting the transferrin receptor.

We have previously reported that an anti-human transferrin receptor IgG3-avidin fusion protein (anti-hTfR IgG3-Av) inhibits the proliferation of an erythroleukemia-cell line. We have now found that anti-hTfR IgG3-Av also inhibits the proliferation of additional human malignant B and plasma cells. Anti-hTfR IgG3-Av induces internalization and rapid degradation of the TfR. These events can be reproduced in cells treated with anti-hTfR IgG3 cross-linked with a secondary Ab,suggesting that they result from increased TfR cross-linking. Confocal microscopy of cells treated with anti-hTfR IgG3-Av shows that the TfR is directed to an intracellular compartment expressing the lysosomal marker LAMP-1. The degradation of TfR is partially blocked by cysteine protease inhibitors. Furthermore,cells treated with anti-hTfR IgG3-Av exhibit mitochondrial depolarization and activation of caspases 9,8,and 3. The mitochondrial damage and cell death can be prevented by iron supplementation,but cannot be fully blocked by a pan-caspase inhibitor. These results suggest that anti-hTfR IgG3-Av induces lethal iron deprivation,but the resulting cell death does not solely depend on caspase activation. This report provides insights into the mechanism of cell death induced by anti-TfR Abs such as anti-hTfR IgG3-Av,a molecule that may be useful in the treatment of B-cell malignancies such as multiple myeloma. View Publication