Educational Materials

Nature Research Round Table: Identifying Acquired and Background Genetic Variants in Human Pluripotent Stem Cells Dr. Florian Merkle from the University of Cambridge talks about the implications of quality control during gene editing, in particular genetic variants that arise during routine culture and genetic variants that might have been acquired during the process of gene editing. This presentation and the following Q&A session were moderated by Dr. Alex Alderton from the Wellcome Trust Sanger Institute.

This presentation was part of a Round Table series titled “Challenges in Ensuring hPSC Quality”, hosted in partnership with Nature Research. Global experts gathered at the Springer Nature headquarters in London, UK, to tackle some of the most pertinent issues impacting the use of human pluripotent stem cells (hPSCs), ranging from fundamental biology research to therapeutic applications. Explore the full series here.

Nature Research Round Table: Defining and Maintaining Pluripotency & hPSC Line Registration and Banking - Panel Discussion In this panel discussion led by Dr. Ludovic Vallier from the Wellcome Trust Sanger Institute and Dr. Joanne Mountford from the University of Glasgow, keynote speakers respond to questions from the forum and evaluate consensuses on topics raised in the previous talks.

This panel discussion was part of a Round Table series titled “Challenges in Ensuring hPSC Quality”, hosted in partnership with Nature Research. Global experts gathered at the Springer Nature headquarters in London, UK, to tackle some of the most pertinent issues impacting the use of human pluripotent stem cells (hPSCs), ranging from fundamental biology research to therapeutic applications. Explore the full series here.

Nature Research Round Table: Standards for Pluripotent Stem Cell Banking Dr. Glyn Stacey, Director of the International Stem Cell Banking Initiative (ISCBI), discusses standards for pluripotent stem cell (PSC) banking with a focus on scientific challenges for PSCs, standardization of ethics, the role of stem cell biobanks, and best practices for cell therapy. This presentation and the following Q&A session were moderated by Dr. Joanne Mountford from the University of Glasgow.

This presentation was part of a Round Table series titled “Challenges in Ensuring hPSC Quality”, hosted in partnership with Nature Research. Global experts gathered at the Springer Nature headquarters in London, UK, to tackle some of the most pertinent issues impacting the use of human pluripotent stem cells (hPSCs), ranging from fundamental biology research to therapeutic applications. Explore the full series here.

Nature Research Round Table: HLA Typing Considerations for Human Pluripotent Stem Cell Banking Dr. David Turner from the Scottish National Blood Transfusion Service discusses immunological considerations around banking of human pluripotent stem cell (hPSC) lines for use in clinical trials. Dr. Turner’s work at the Histocompatibility and Immunogenetics (H&I) Laboratory involves donor-patient HLA-matching for clinical transplants. This presentation and the following Q&A session were moderated by Dr. Joanne Mountford from the University of Glasgow.

This presentation was part of a Round Table series titled “Challenges in Ensuring hPSC Quality”, hosted in partnership with Nature Research. Global experts gathered at the Springer Nature headquarters in London, UK, to tackle some of the most pertinent issues impacting the use of human pluripotent stem cells (hPSCs), ranging from fundamental biology research to therapeutic applications. Explore the full series here.

Note: Some original data from this presentation has been omitted to abide by copyright rules.

Nature Research Round Table: Regulations Around Human Induced Pluripotent Stem Cell Registration Dr. Andreas Kurtz from the Berlin-Brandenburg Center for Regenerative Therapies discusses data associated with human pluripotent stem cells (hPSCs) as well as the benefits of and regulations around data registration for cell line characterization. Since 2010, Dr. Kurtz has managed hPSCreg, a freely accessible global registry for human hPSC lines. This presentation and the following Q&A session were moderated by Dr. Joanne Mountford from the University of Glasgow.

This presentation was part of a Round Table series titled “Challenges in Ensuring hPSC Quality”, hosted in partnership with Nature Research. Global experts gathered at the Springer Nature headquarters in London, UK, to tackle some of the most pertinent issues impacting the use of human pluripotent stem cells (hPSCs), ranging from fundamental biology research to therapeutic applications. Explore the full series here.

Nature Research Round Table: Maintenance of Human Pluripotent Stem Cells In Vitro Dr. Tenneille Ludwig from the WiCell Research Institute provides information on in vitro culture systems for ES and iPS cell maintenance. The WiCell Research Institute is widely known for their human pluripotent stem cell (hPSC) banking services, with over 1,350 cell lines available to researchers around the globe. This presentation and the following Q&A session were moderated by Dr. Ludovic Vallier from the Wellcome Trust Sanger Institute.

This presentation was part of a Round Table series titled “Challenges in Ensuring hPSC Quality”, hosted in partnership with Nature Research. Global experts gathered at the Springer Nature headquarters in London, UK, to tackle some of the most pertinent issues impacting the use of human pluripotent stem cells (hPSCs), ranging from fundamental biology research to therapeutic applications. Explore the full series here.

Nature Research Round Table: Pluripotency Tests Dr. Peter Andrews discusses what the term “pluripotency” means in relation to ES and iPS cells, how the term has evolved, and how to assess pluripotent potential by comparing existing assays. Dr. Andrews is co-director of the Centre for Stem Cell Biology at the University of Sheffield. This presentation and the following Q&A session were moderated by Dr. Ludovic Vallier from the Wellcome Trust Sanger Institute.

This presentation was part of a Round Table series titled “Challenges in Ensuring hPSC Quality”, hosted in partnership with Nature Research. Global experts gathered at the Springer Nature headquarters in London, UK, to tackle some of the most pertinent issues impacting the use of human pluripotent stem cells (hPSCs), ranging from fundamental biology research to therapeutic applications. Explore the full series here.

Note: Some original data from this presentation has been omitted to abide by copyright rules.

How to Count hPSC Aggregates to Determine Plating Density for Maintenance and Differentiation Step-by-step instructions for counting human pluripotent stem cell (hPSC) aggregates to determine and adjust the plating densities for hPSC maintenance and differentiation

Isolation of Mouse CD45 Positive Leukocytes from Tissues

Automated Lymphocyte Isolation for the Flow Cytometry Crossmatch Assay

Bone Marrow Skeletal Progenitors and Bone Diseases

Cellular Organization and Biology of the Respiratory System Overview of the organization and function of different parts of the respiratory system