Educational Materials
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Optimized Workflows for High-Efficiency Genome Editing in Stem and Primary Cell Types CRISPR-Cas genome editing in cell culture systems is a powerful technique for disease modeling and the development of cellular therapies. Compared to work with immortalized cell lines, genome editing of stem and primary cells presents unique challenges, including issues related to efficient delivery and expression of CRISPR machinery, clonogenicity, and cytotoxicity. In this webinar, our in-house expert, Dr. Ashley Watson, discusses optimized workflows for CRISPR-Cas9 genome editing in human pluripotent stem cells and primary T cells.
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Development, Compatibility, and Applications of mTeSR™ Plus; an Enhanced Medium for the Maintenance of Human Pluripotent Stem Cells (hPSCs) High quality hPSC maintenance cultures are the foundation for robust and reproducible downstream applications. In this talk, presented at the 2019 ISSCR Annual Meeting in Los Angeles, in-house scientist Dr. Melanie Kardel is joined by Loren Ornelas and Dr. Dhruv Sareen from the Cedars-Sinai Induced Pluripotent Stem Cell (iPSC) Core. This talk features an overview of mTeSR™ Plus, core services, highlights key collaborations, and describes the core facility’s experience with mTeSR™ Plus including cell line transition and quality control.