Methods Library
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Large-Scale Expansion of Human Pluripotent Stem Cells in 3D Suspension Culture with mTeSR™3D Medium Features and benefits of mTeSR™3D, a specialized medium for expansion of human pluripotent stem cells in suspension culture, including the novel fed-batch culture system
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Setting Up Controls and Gating for ALDHbr Assay Analysis Find everything you need to know about preparing the proper single color controls, FMOs and gates for performing flow cytometry analysis of the ALDHbr assay
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How to Prepare and Plate Semi-Solid Medium Steps involved with preparing and plating methylcellulose-based semi-solid media (e.g. MethoCult™ and ClonaCell™)
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Culture and Analysis of Hematopoietic Progenitor Cells in CFU Assays Use these tips to better understand how the right medium can make the analysis of your hematopoietic CFU assays clearer
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Maturation of Culture-Expanded Erythroid Progenitor Cells Technical tip from our dedicated team of Product and Scientific Support specialists
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Serum-Free Expansion of CD34+ Cells with SFEM II and UM729 Explore the use of StemSpan™ SFEM II medium, StemSpan™ CD34+ Expansion Supplement and small molecules UM171 or UM729 to enhance expansion of HSPCs
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Human Hematopoietic Stem and Progenitor Cell Markers and Phenotyping Panels Technical tip from our dedicated team of Product and Scientific Support specialists
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Cord Blood CD34+ Cell Isolation Technical tip from our dedicated team of Product and Scientific Support specialists
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How to Reprogram Fibroblasts into Human Induced Pluripotent Stem (iPS) Cells Using ReproRNA™-OKSGM How to reprogram fibroblasts in using ReproRNA™-OKSGM is a non-integrating, self-replicating RNA reprogramming vector for generating human induced pluripotent stem (iPS) cells