Methods Library
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Customizable Semi-Solid Cloning of Mammalian Cells in 96-Well Plates Using ClonaCell™ FLEX Medium ClonaCell™ FLEX 96-well plate semi-solid cloning protocol, including plating, liquid medium overlay, screening, and colony picking
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Flow Cytometry Analysis of ALDH Bright Cells with the ALDEFLUOR™ Assay Kit The ALDEFLUOR™ fluorescent reagent system has supported over 200 publications to date
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Tapping Technique for Passaging Pluripotent Stem Cells Using ReLeSR™ Selection and Passaging Reagent Tip for the successful tapping technique when using ReLeSR™
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CFU Assay Instructions for Global Proficiency Testing Programs Learn to perform the colony-forming unit (CFU) assay for Proficiency Testing programs
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The Easy 50 EasySep™ Magnet: Portable Large-Volume Cell Separation In 25 Minutes Learn to use the portable yet powerful Easy 50 EasySep™ magnet to obtain purified cells from large volumes & a variety of sources
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How to Isolate PBMCs from Whole Blood Using the SepMate™ PBMC Isolation Tubes: 15-Minute Protocol Easy and rapid procedure for isolating peripheral blood mononuclear cells (PBMCs) from whole blood or bone marrow using the unique SepMate™ PBMC isolation tubes
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Optimization of ALDEFLUOR™ Protocol Learn how STEMCELL Technologies' scientists doubled the signal strength in mammary epithelial cells with a few simple protocol modifications using ALDEFLUOR™ to detect Aldehyde Dehydrogenase bright (ALDHbr) cells
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How to Isolate Cells Directly from Whole Blood Using the EasySep™ Purple/Silver Magnets How to isolate highly purified immune cells directly from whole blood and takes as little as 20 minutes from start to finish
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How to Isolate Cells Directly from Whole Blood - EasySep™ Direct Protocol, EasyEights Magnet How to isolate highly purified immune cells from up to 16 whole blood samples at once with EasySep™ Direct on the EasyEights EasySep™ Magnet and the protocol takes as little as 25 minutes from start to finish
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How to Isolate Cells Directly from Whole Blood Using the Easy 50 EasySep™ Magnet: 30-Minute Protocol How to isolate highly purified immune cells directly from whole blood samples of up to 30 mL with and the protocol takes as little as 30 minutes from start to finish
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ClonaCell™-HY Procedure Selection and growth of mouse hybridomas to generate mouse monoclonal antibodies using ClonaCell™-HY including preparation of myeloma cells and splenocytes, fusion, selection and cloning, and harvesting
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How to Deplete Red Blood Cells from Small Volume Samples with HetaSep™ How to use an erythrocyte aggregation agent to remove red blood cells (RBCs) from small volume blood samples prior to colony-forming unit (CFU) assays