Methods Library

Assessment of Your Starting Sample and Calculation of Recovery from Cell Isolation Procedures Setting aside a small portion of your starting sample allows you to assess the frequency of desired cells in the sample prior to isolation
Isolate Multiple Cell Types from a Single Sample with Sequential Isolation Sequential separation, for higher recovery of purified cells from limited sample size and isolation of multiple cell types, is ideal for chimerism analysis
Importance of Using an FcR Blocker During Cell Separation Procedures EasySep™ Other Species indirect selection kits can be used to isolate cells from any species
Acquiring DNA or RNA from Positively Selected Cells Cells positvely selected with the EasySep™ system do not interfere with most downstream applications
Workflows for Culturing Primary-Derived Neural Stem and Progenitor Cells NeuroCult™, developed to support the proliferation of NSCs in neurosphere suspension cultures, is also fully compatible with adherent culture systems
Cryopreservation of Neurospheres Freeze intact neurospheres in the appropriate species-specific complete NeuroCult™ Proliferation Medium using controlled rate freezing containers
Identification of Human Neural Stem and Progenitor Cells Derived from Pluripotent Cells Technical tip from our dedicated team of Product and Scientific Support specialists
Neural Induction of hPSCs Using the Monolayer Method STEMdiff™ Neural Induction Media (NIM) is a serum-free, defined medium optimized for embryoid body formation from hPSCs
Enzyme-Free Passaging and Generation of Single Cells from Human ES and iPS Cells with Gentle Cell Dissociation Reagent Gentle Cell Dissociation Reagent (GCDR) is enzyme-free, animal component-free and allows great expansion (12-40 fold) of human ES and iPS cell cultures
Critical Tips for Establishing and Maintaining Mouse Intestinal Organoid Culture Technical tip from our dedicated team of Product and Scientific Support specialists
Considerations for Flow Cytometry Gating To achieve accurate results, it is important to use an optimal gating strategy when assessing cell samples via flow cytometry
Reducing Platelet Contamination in Blood Samples Platelets (thrombocytes), cell fragments derived from megakaryocytes, are used in normal blood clotting, and must be handled gently to prevent activation