Tech Tips & Protocols

Performing Total Nucleated Cell Counts with a Hemocytometer Technical tip from our dedicated team of Product and Scientific Support specialists

Working with Small Sample Volumes or Low Start Cell Numbers Using the Purple EasySep™ and EasyPlate™ EasySep™ Platforms Technical tip from our dedicated team of Product and Scientific Support specialists

Processing Multiple Samples at One Time with the EasyEights™ Magnet The EasyEights™ EasySep™ Magnet is for cell separation procedures using selected EasySep™ reagents when simultaneously processing multiple samples

Improving Cell Recovery with EasySep™ Negative Selection Kits Technical tip from our dedicated team of Product and Scientific Support specialists

Isolate Virtually Any Cell Type Using EasySep™ or EasySep™ Release Indirect Selection Kits Use any biotinylated, FITC-, PE- or APC-conjugated antibody to label your desired cells and mix with EasySep™ reagents to obtain highly purified cells

Using EasySep™ Positive Selection Kits for Cell Depletion The EasySep™ system allows you to use a positive selection kit to deplete the labeled cell type, by making changes to the separation scheme of the protocol

DNase I Treatment for Clumpy Cell Suspensions To reduce cell clumping and minimize presence of free-floating DNA fragments, we recommend adding DNase I Solution (Catalog #07900) to the cell suspension

Isolating Cells from Atypical Samples Technical tip from our dedicated team of Product and Scientific Support specialists

Assessment of Your Starting Sample and Calculation of Recovery from Cell Isolation Procedures Setting aside a small portion of your starting sample allows you to assess the frequency of desired cells in the sample prior to isolation

Isolate Multiple Cell Types from a Single Sample with Sequential Isolation Sequential separation, for higher recovery of purified cells from limited sample size and isolation of multiple cell types, is ideal for chimerism analysis

Importance of Using an FcR Blocker During Cell Separation Procedures EasySep™ Other Species indirect selection kits can be used to isolate cells from any species

Acquiring DNA or RNA from Positively Selected Cells Cells positvely selected with the EasySep™ system do not interfere with most downstream applications