Hybridoma and Mammalian Cell Line Development
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How to Prepare and Plate Semi-Solid Medium Steps involved with preparing and plating methylcellulose-based semi-solid media (e.g. MethoCult™ and ClonaCell™)
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Video Tutorial: Probability of Monoclonality Describes statistical models that can be used to estimate the probability of monoclonality for limiting dilution cloning and semi-solid cloning
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Semi-Solid Cloning of CHO Cells Using ClonaCell™-CHO Medium Procedure for semi-solid cloning, including preparation of the cells, plating, colony picking, and expansion
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How to Perform Semi-Solid Cloning of CHO Cell Lines in 96-Well Plates Using ClonaCell™-CHO Medium This video outlines the ClonaCell™-CHO 96-well plate semi-solid cloning protocol, including plating, liquid overlay, screening, and colony picking
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Mammalian Cell Cloning Procedure: Customizable Semi-Solid Cloning with ClonaCell™ FLEX Medium This video outlines the ClonaCell™ FLEX procedure for semi-solid cloning, including preparation of the cells, plating, colony picking, and expansion
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Why Use Semi-Solid Medium for Cloning Hybridomas and CHO Cells Cloning hybridomas and CHO cells can be done faster and more efficiently using semi-solid cloning, compared to traditional cloning techniques
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How to Generate Hybridomas in 96-Well Plates Using ClonaCell™-HY Semi-Solid Cloning Medium Adaptation of the ClonaCell™-HY semi-solid cloning protocol to a 96-well plate format
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Customizable Semi-Solid Cloning of Mammalian Cells in 96-Well Plates Using ClonaCell™ FLEX Medium ClonaCell™ FLEX 96-well plate semi-solid cloning protocol, including plating, liquid medium overlay, screening, and colony picking
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ClonaCell™-HY Procedure Selection and growth of mouse hybridomas to generate mouse monoclonal antibodies using ClonaCell™-HY including preparation of myeloma cells and splenocytes, fusion, selection and cloning, and harvesting