Cell Line Development
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Video Tutorial: Probability of Monoclonality Describes statistical models that can be used to estimate the probability of monoclonality for limiting dilution cloning and semi-solid cloning
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Semi-Solid Cloning of CHO Cells Using ClonaCell™-CHO Medium Procedure for semi-solid cloning, including preparation of the cells, plating, colony picking, and expansion
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Mammalian Cell Cloning Procedure: Customizable Semi-Solid Cloning with ClonaCell™ FLEX Medium This video outlines the ClonaCell™ FLEX procedure for semi-solid cloning, including preparation of the cells, plating, colony picking, and expansion
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Customizable Semi-Solid Cloning of Mammalian Cells in 96-Well Plates Using ClonaCell™ FLEX Medium ClonaCell™ FLEX 96-well plate semi-solid cloning protocol, including plating, liquid medium overlay, screening, and colony picking
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ClonaCell™-HY Procedure Selection and growth of mouse hybridomas to generate mouse monoclonal antibodies using ClonaCell™-HY including preparation of myeloma cells and splenocytes, fusion, selection and cloning, and harvesting