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STEMdiff™ 小胶质细胞成熟试剂盒

用于将人胚胎干细胞(ES)和诱导多能干细胞(iPS)来源的小胶质细胞前体分化成熟为小胶质细胞的成熟试剂盒。

产品号 #(选择产品)

产品号 #100-0020_C

用于将人胚胎干细胞(ES)和诱导多能干细胞(iPS)来源的小胶质细胞前体分化成熟为小胶质细胞的成熟试剂盒。

产品优势

  • 超过90%的造血祖细胞(HPCs)可成功分化为小胶质细胞。
  • 与使用 STEMdiff™ 造血细胞试剂盒生成的 HPCs 兼容,并可与 STEMdiff™ 神经类器官共培养。
  • 可分化生成几乎不含污染性巨噬细胞或单核细胞的小胶质细胞。
  • 通过该试剂盒生成的小胶质细胞具备吞噬和激活功能。
  • 所需材料以简便易用的形式提供。

产品组分包括

  • STEMdiff™ 小胶质细胞成熟试剂盒(目录号 #100-0020)
    • STEMdiff™ 小胶质细胞基础培养基,90 mL
    • STEMdiff™ 小胶质细胞补充剂 1,10 mL
    • STEMdiff™ 小胶质细胞补充剂 2,400 μL
    • STEMdiff™ 小胶质细胞补充剂 3,400 μL
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总览

STEMdiff™ 小胶质细胞培养系统包括 STEMdiff™ 小胶质细胞分化试剂盒和 STEMdiff™ 小胶质细胞成熟试剂盒。这两种试剂盒结合使用,用于分化和成熟采用 STEMdiff™ 造血试剂盒(货号 #05310)获得的人类多能干细胞 (hPSC) 衍生小胶质细胞。
该系统基于 Mathew Blurton-Jones 实验室的方案(Abud 等,2017),生成的细胞为高纯度的小胶质细胞群(>80% CD45/CD11b 阳性,>50% TREM2 阳性小胶质细胞;形态上明显不同的单核细胞或巨噬细胞占比 <20%)。

使用该系列产品获得的细胞可作为研究神经炎症、人类神经系统发育与疾病、共培养实验以及毒性测试的多功能工具。

使用该系列产品获得的细胞可作为研究神经炎症、人类神经系统发育与疾病、共培养实验以及毒性测试的多功能工具。

使用该系列产品获得的细胞可作为研究神经炎症、人类神经系统发育与疾病、共培养实验以及毒性测试的多功能工具。

使用该系列产品获得的细胞可作为研究神经炎症、人类神经系统发育与疾病、共培养实验以及毒性测试的多功能工具。

亚型
专用培养基
 
细胞类型
造血细胞,PSC衍生,小胶质细胞,神经细胞,PSC衍生
 
种属

 
应用
细胞培养,分化
 
品牌
STEMdiff
 
研究领域
疾病建模,药物发现和毒理检测,免疫,神经科学
 
制剂类别
无血清
 

实验数据

Experimental protocol schematic for generating microglial precursors from hPSC-derived hematopoietic progenitor cells.

Figure 1. Schematic for the STEMdiff™ Microglia Culture System Protocol

Functional microglia can be generated in 4-10 days from microglial precursors after 24 days in STEMdiff™ Microglia Differentiation Medium. For the differentiation of microglial precursors from hPSC-derived hematopoietic progenitor cells, see the PIS.

Bulk RNA-seq heatmap showing that STEMdiff™ Microglia express disease-relevant genes similar to those from popular protocols.

Figure 2. Microglia Generated with STEMdiff™ Microglia Culture System Express Disease-Relevant Genes Similar to Those from Published Differentiation and Maturation Protocols

Bulk RNA-seq datasets were extracted from 8 different publications that generated hPSC- (iMGL) and primary- (MGL) derived microglia and their transcriptional profiles compared to data from microglia generated with STEMdiff™ Microglia Culture System. The heat map displays absolute expression levels for select genes associated with Alzheimer’s disease, Parkinson’s disease, and viral encephalitis. Significant differences in gene expression between microglia generated with STEMdiff™ Microglia Culture System and any of the other 3 groups were identified by differential gene expression analysis. *= p<0.05 (DEseq2, adjusted). hPSC = human pluripotent stem cell.

Graphs demonstrating that STEMdiff™ Microglia release cytokines in response to LPS stimulation, compared to control.

Figure 3. Microglia Generated with STEMdiff™ Microglia Culture System Release Cytokines in Response to Inflammatory Signals

Microglia were generated using the STEMdiff™ Microglia Culture System and stimulated with 100 ng/mL LPS for 24 hours. The release of pro-inflammatory (TNFα, IL-6, IFN-γ, IL-1β, GM-CSF, IL-12p70, IL-2, IL-8) and anti-inflammatory (IL-10) cytokines were measured by MSD. The microglia release cytokines in response to LPS treatment, as expected. *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001. LPS = lipopolysaccharide; MSD = Meso Scale Discovery.

Time lapse of functional microglia phagocytosing bioindicator particles in culture. Over time, cells turn red and aggregate.

Figure 4. STEMdiff™ Microglia Culture System Generates Functional Microglia Capable of Phagocytosis at Day 34

Microglia taking up pH-sensitive bioindicator particles at a concentration of 250 μg/mL (small dots) were measured over a 12-hour time period with live cell imaging. As the particles are phagocytosed, the cells turn red. Over time, the number of small dots decreased, and the red cells increased in number and aggregated. Scale bar = 100μm.

Figure 5. PSC-Derived Microglia Incorporate into Brain Organoids After 10 Days and Display an Activated Morphology upon Injury.

(A) Representative microglia and brain organoid co-cultures after 10 days, stained with IBA1 for microglia (green) and MAP2 for neurons (magenta). The microglia integrate among the neurons and display an unactivated morphology with extended processes (arrow). (B) The microglia display an activated amoeboid morphology upon injury as shown by IBA1 staining.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0020
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0020
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0020
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-0020
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Catalog #
100-0020
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。

相关材料与文献

技术资料 (20)

更多信息

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种属 Human
配方类别 Serum-Free
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