EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #100-1364_C
无动物成分的补充剂，用于提高人多能干细胞（hPSC）衍生视网膜色素上皮细胞的存活率和贴壁能力
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专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
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STEMdiff™-XF RPE Maturation MediumSerum-free and xeno-free medium for generating fully functional and mature hPSC-derived retinal pigment epithelium
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STEMdiff™-ACF RPE Differentiation KitAnimal component-free differentiation kit for the generation of immature retinal pigment epithelium from human pluripotent stem cells
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Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells
总览
通过在STEMdiff™-XF RPE 成熟培养基 中添加 STEMdiff™-ACF RPE 铺板补充剂，可显著提高解离、复苏和重新铺板后人多能干细胞 (hPSC) 衍生的视网膜色素上皮 (RPE) 细胞的存活率。
此补充剂旨在提高使用 STEMdiff™-ACF RPE分化试剂盒生成的 hPSC 衍生 RPE细胞的实验通量。
请将本补充剂与以下兼容的 STEMdiff™ RPE 产品配合使用，以构建完整且优化的工作流程：
STEMdiff™-ACF RPE 分化试剂盒
STEMdiff™-XF RPE 成熟培养基
如您计划将此产品用于商业或临床用途，请与我们联系。
通过在STEMdiff™-XF RPE 成熟培养基 中添加 STEMdiff™-ACF RPE 铺板补充剂，可显著提高解离、复苏和重新铺板后人多能干细胞 (hPSC) 衍生的视网膜色素上皮 (RPE) 细胞的存活率。
此补充剂旨在提高使用 STEMdiff™-ACF RPE分化试剂盒生成的 hPSC 衍生 RPE细胞的实验通量。
请将本补充剂与以下兼容的 STEMdiff™ RPE 产品配合使用，以构建完整且优化的工作流程：
STEMdiff™-ACF RPE 分化试剂盒
STEMdiff™-XF RPE 成熟培养基
如您计划将此产品用于商业或临床用途，请与我们联系。
此补充剂旨在提高使用 STEMdiff™-ACF RPE分化试剂盒生成的 hPSC 衍生 RPE细胞的实验通量。
请将本补充剂与以下兼容的 STEMdiff™ RPE 产品配合使用，以构建完整且优化的工作流程：
STEMdiff™-ACF RPE 分化试剂盒
STEMdiff™-XF RPE 成熟培养基
如您计划将此产品用于商业或临床用途，请与我们联系。
此补充剂旨在提高使用 STEMdiff™-ACF RPE分化试剂盒生成的 hPSC 衍生 RPE细胞的实验通量。
请将本补充剂与以下兼容的 STEMdiff™ RPE 产品配合使用，以构建完整且优化的工作流程：
STEMdiff™-ACF RPE 分化试剂盒
STEMdiff™-XF RPE 成熟培养基
如您计划将此产品用于商业或临床用途，请与我们联系。
Cell Type
Neural Cells, PSC-Derived, Pluripotent Stem Cells
Application
Cell Culture
Brand
STEMdiff
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience, Cell Therapy Development
实验数据
Figure 1. Workflow for the Differentiation of hPSCs into Retinal Pigment Epithelium (RPE) with STEMdiff™ RPE Culture System
hPSC colonies, previously harvested and seeded as clumps, are put directly into the medium provided in STEMdiff™-ACF RPE Differentiation Kit. Seeding into STEMdiff™ RPE Differentiation Medium A induces cells toward immature RPE. A full medium change is performed on Day 1 with fresh STEMdiff™ RPE Differentiation Medium A and then on Day 2 with STEMdiff™ RPE Differentiation Medium B. On Days 4 and 6, medium changes are performed with fresh STEMdiff™ RPE Differentiation Medium C. On Days 7 and every second day thereafter a medium change is performed with fresh STEMdiff™ RPE Differentiation Medium D. On Day 14, immature RPE cells are enzymatically harvested and subcultured in STEMdiff™-XF RPE Maturation Medium with the addition of STEMdiff™-ACF RPE Plating Supplement from Days 14 to 21 to improve survival after passaging. RPE cells begin to mature over the course of 5 weeks of culture in STEMdiff™-XF RPE Maturation Medium and become fully matured by Day 49, possessing key characteristics such as polygonal shape, polarization, pigmentation and phagocytosis.
Figure 2. STEMdiff™ Retinal Pigment Epithelial (RPE) Plating Supplement Improves Attachment and Morphology of Replated hPSC-Derived Immature RPE Cells Derived From 4 hPSC Lines
(A) Four different cell lines of hPSC-derived immature RPE cells were replated in either STEMdiff™-XF RPE Maturation Medium alone, or (B) with added STEMdiff™-ACF RPE Plating Supplement (RPE Plating Medium). (A) Representative images of replated hPSC-derived immature RPE cells are shown 1 Day after replating. (B) hPSC-derived immature RPE cells exhibit improved attachment when replated with RPE Plating Medium. Data shown as mean + SEM; n = 3. (C) Plating Efficiency was measured across the four cell lines following replating showing a 3-4 fold increase with the STEMdiff™ RPE-ACF Plating Supplement.
Figure 3. hPSC-Derived Immature Retinal Pigment Epithelial (RPE) Cells Replated Using STEMdiff™-ACF RPE Plating Supplement Show Increased Cell Survival and Improved Morphology Post Cryopreservation
(A) Four different cell lines of cryopreserved hPSC-derived immature RPE cells were thawed and replated in either STEMdiff™-XF RPE Maturation Medium alone, or (B) with STEMdiff™-ACF RPE Plating Supplement (RPE Plating Medium). Representative images show cells 1 Day post-thaw. Post-thaw, hPSC-derived immature RPE cells exhibited improved attachment when replated with RPE Plating Medium. Data shown as mean + SEM; n = 3. (C) Post-Thaw Plating Efficiency was measured across the four cell lines following replating showing a 3-4 fold increase with the STEMdiff™-ACF RPE Plating Supplement.
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
STEMdiff™ RPE Plating Supplement Catalog #
100-1364 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
STEMdiff™ RPE Plating Supplement Catalog #
100-1364 Lot #
All Language
English 应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
Research Area
Workflow Stages
Workflow Stages for Human Pluripotent Stem Cell Research
Workflow Stages for hPSC-Derived Sensory Research
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