EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #100-1364_C
无动物成分的补充剂，用于提高人多能干细胞（hPSC）衍生视网膜色素上皮细胞的存活率和贴壁能力
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总览
通过在STEMdiff™-XF RPE 成熟培养基 中添加 STEMdiff™-ACF RPE 铺板补充剂，可显著提高解离、复苏和重新铺板后人多能干细胞 (hPSC) 衍生的视网膜色素上皮 (RPE) 细胞的存活率。
此补充剂旨在提高使用 STEMdiff™-ACF RPE分化试剂盒生成的 hPSC 衍生 RPE细胞的实验通量。
请将本补充剂与以下兼容的 STEMdiff™ RPE 产品配合使用，以构建完整且优化的工作流程：
STEMdiff™-ACF RPE 分化试剂盒
STEMdiff™-XF RPE 成熟培养基
如您计划将此产品用于商业或临床用途，请与我们联系。
细胞类型
神经细胞，PSC衍生，多能干细胞
应用
细胞培养
品牌
STEMdiff
研究领域
疾病建模，药物发现和毒理检测，神经科学，细胞治疗开发
实验数据
Figure 1. Workflow for the Differentiation of hPSCs into Retinal Pigment Epithelium (RPE) with STEMdiff™ RPE Culture System
hPSC colonies, previously harvested and seeded as clumps, are put directly into the medium provided in STEMdiff™-ACF RPE Differentiation Kit. Seeding into STEMdiff™ RPE Differentiation Medium A induces cells toward immature RPE. A full medium change is performed on Day 1 with fresh STEMdiff™ RPE Differentiation Medium A and then on Day 2 with STEMdiff™ RPE Differentiation Medium B. On Days 4 and 6, medium changes are performed with fresh STEMdiff™ RPE Differentiation Medium C. On Days 7 and every second day thereafter a medium change is performed with fresh STEMdiff™ RPE Differentiation Medium D. On Day 14, immature RPE cells are enzymatically harvested and subcultured in STEMdiff™-XF RPE Maturation Medium with the addition of STEMdiff™-ACF RPE Plating Supplement from Days 14 to 21 to improve survival after passaging. RPE cells begin to mature over the course of 5 weeks of culture in STEMdiff™-XF RPE Maturation Medium and become fully matured by Day 49, possessing key characteristics such as polygonal shape, polarization, pigmentation and phagocytosis.
Figure 2. STEMdiff™ Retinal Pigment Epithelial (RPE) Plating Supplement Improves Attachment and Morphology of Replated hPSC-Derived Immature RPE Cells Derived From 4 hPSC Lines
(A) Four different cell lines of hPSC-derived immature RPE cells were replated in either STEMdiff™-XF RPE Maturation Medium alone, or (B) with added STEMdiff™-ACF RPE Plating Supplement (RPE Plating Medium). (A) Representative images of replated hPSC-derived immature RPE cells are shown 1 Day after replating. (B) hPSC-derived immature RPE cells exhibit improved attachment when replated with RPE Plating Medium. Data shown as mean + SEM; n = 3. (C) Plating Efficiency was measured across the four cell lines following replating showing a 3-4 fold increase with the STEMdiff™ RPE-ACF Plating Supplement.
Figure 3. hPSC-Derived Immature Retinal Pigment Epithelial (RPE) Cells Replated Using STEMdiff™-ACF RPE Plating Supplement Show Increased Cell Survival and Improved Morphology Post Cryopreservation
(A) Four different cell lines of cryopreserved hPSC-derived immature RPE cells were thawed and replated in either STEMdiff™-XF RPE Maturation Medium alone, or (B) with STEMdiff™-ACF RPE Plating Supplement (RPE Plating Medium). Representative images show cells 1 Day post-thaw. Post-thaw, hPSC-derived immature RPE cells exhibited improved attachment when replated with RPE Plating Medium. Data shown as mean + SEM; n = 3. (C) Post-Thaw Plating Efficiency was measured across the four cell lines following replating showing a 3-4 fold increase with the STEMdiff™-ACF RPE Plating Supplement.
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
STEMdiff™ RPE Plating Supplement Catalog #
100-1364 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
STEMdiff™ RPE Plating Supplement Catalog #
100-1364 Lot #
All Language
English 应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
Research Area
Workflow Stages
Workflow Stages for Human Pluripotent Stem Cell Research
Workflow Stages for hPSC-Derived Sensory Research
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