Figure 1. STEMprep™ Mouse Liver Dissociation Kit Achieves High Cell Viability and Consistent Yield
Mouse liver tissue was processed into single-cell suspensions using the STEMprep™ Mouse Liver Dissociation Kit and the STEMprep™ Tissue Dissociator or an alternative automated system. (A) Viability of total nucleated cells. (B) Yield of viable cells per whole liver tissue (900 - 2000 mg). (C and D) Proportion and yield of F4/80+CLEC4F+ Kupffer cells, and CD31+ liver sinusoidal endothelial cells. Viability, yield, and subset composition were assessed by flow cytometry. Red blood cells were lysed with ammonium chloride solution before subset analysis. Data are presented as mean ± SD (n = 15 - 37).
Figure 2. STEMprep™-Processed Mouse Kupffer Cells Maintain Phagocytic Function
Mouse liver tissue was dissociated into single-cell suspensions using the STEMprep™ Mouse Liver Dissociation Kit and the STEMprep™ Tissue Dissociator, or an alternative automated system. (A) Kupffer cells were isolated from the single-cell suspensions using the EasySep™ Mouse F4/80 Positive Selection Kit. (B) The isolated F4/80+CLEC4F+ cells were incubated for 2h in the presence of pHrodo™ Green-conjugated E. coli BioParticles™ (BP) at 2 - 8°C (Cold) or 37°C. The fluorescence of phagocytosed BP was measured by flow cytometry. Data are presented as mean ± SD (n = 6).
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EasySep™小鼠TIL（CD45）正选试剂盒
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