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EasySep™ 小鼠F4/80正选试剂盒

从小鼠脾细胞、肺组织和腹腔灌洗液中免疫磁珠正选小鼠F4/80+细胞
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产品号 #（选择产品）

产品号 #100-0659_C

从小鼠脾细胞、肺组织和腹腔灌洗液中免疫磁珠正选小鼠F4/80+细胞

产品优势

  • 操作简单、快捷，且无需分离柱
  • 纯度高达90%
  • 分选得到的细胞无荧光标记

产品组分包括

  • EasySep™小鼠F4/80阳性选择组分A， 0.3 mL
  • EasySep™小鼠F4/80阳性选择组分B， 0.6 mL
  • EasySep™Dextran RapidSpheres™50100,2 × 1 mL
  • 鼠标FcR PolyBlock（目录#300-0902），1.2 mL
main product photo
New format, same high quality! You may notice that your kit contents and packaging look slightly different from previous orders. We are currently updating the format of select EasySep™ Mouse kits to include a Mouse FcR blocker instead of Normal Rat Serum. With this change, all components will now be shipped in a single package, while providing the same cell isolation performance as before.
专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

使用EasySep™小鼠F4/80正选试剂盒，通过免疫磁珠正选技术，可轻松从小鼠脾细胞、肺组织和腹腔灌洗液样本中分离高纯度小鼠F4/80+细胞。EasySep™技术结合单克隆抗体的特异性和无柱磁分选系统的简便性，已在发表的研究中广泛应用超过20年。

在该EasySep™正选流程中，目标细胞通过识别F4/80的抗体复合物和磁珠标记，并通过EasySep™磁极进行分选。只需简单倾倒或吸取以弃去非目的细胞，而目的细胞则保留在试管中。在短至24分钟的磁分选后，目的F4/80+细胞可立即用于流式细胞术、培养和基于细胞的实验等下游应用。

了解更多关于EasySep™免疫磁珠分选技术的工作原理。探索更多为您实验流程优化的其它产品，包括培养基、补充剂、抗体等。

在该EasySep™正选流程中，目标细胞通过识别F4/80的抗体复合物和磁珠标记，并通过EasySep™磁极进行分选。只需简单倾倒或吸取以弃去非目的细胞，而目的细胞则保留在试管中。在短至24分钟的磁分选后，目的F4/80+细胞可立即用于流式细胞术、培养和基于细胞的实验等下游应用。

了解更多关于EasySep™免疫磁珠分选技术的工作原理。探索更多为您实验流程优化的其它产品，包括培养基、补充剂、抗体等。

在该EasySep™正选流程中，目标细胞通过识别F4/80的抗体复合物和磁珠标记，并通过EasySep™磁极进行分选。只需简单倾倒或吸取以弃去非目的细胞，而目的细胞则保留在试管中。在短至24分钟的磁分选后，目的F4/80+细胞可立即用于流式细胞术、培养和基于细胞的实验等下游应用。

了解更多关于EasySep™免疫磁珠分选技术的工作原理。探索更多为您实验流程优化的其它产品，包括培养基、补充剂、抗体等。

在该EasySep™正选流程中，目标细胞通过识别F4/80的抗体复合物和磁珠标记，并通过EasySep™磁极进行分选。只需简单倾倒或吸取以弃去非目的细胞，而目的细胞则保留在试管中。在短至24分钟的磁分选后，目的F4/80+细胞可立即用于流式细胞术、培养和基于细胞的实验等下游应用。

了解更多关于EasySep™免疫磁珠分选技术的工作原理。探索更多为您实验流程优化的其它产品，包括培养基、补充剂、抗体等。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyPlate™ EasySep™ Magnet (Catalog #18102) • EasyEights™ EasySep™ Magnet (Catalog #18103)
 
亚型
细胞分选试剂盒
 
细胞类型
巨噬细胞
 
种属
小鼠
 
样本来源
Lung，其它细胞系，Spleen
 
筛选方法
Positive
 
应用
细胞分选
 
品牌
EasySep
 
研究领域
免疫，感染性疾病(传染病)
 

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实验数据

FACS plot showing cell populations from lung tissue pre- and post-cell isolation using the EasySep™ Mouse F4/80 Positive Selection Kit

Figure 1. Typical EasySep™ Mouse F4/80 Positive Cell Isolation Profile from Lung Tissue

Starting with a naïve mouse lung single-cell suspension, the F4/80+ cell content of the isolated fraction is typically 94.3 ± 2.8% (mean ± SD) using the purple EasySep™ magnet. In the above example, the purities of the start and final isolated fractions are 26.3% and 95.3%, respectively.

Typical Profile for F4/80+ Cells Isolated from Peritoneal Lavage Using the EasySep™ Mouse F4/80 Positive Selection Kit

Figure 2. Typical EasySep™ Mouse F4/80 Positive Cell Isolation Profile from Peritoneal Lavage

Starting with lavage cells from the peritoneal cavity of a naïve mouse, the F4/80+ cell content of the isolated fraction is typically 97.0 ± 0.4% (mean ± SD) using the purple EasySep™ magnet. In the above example, the purities of the start and final isolated fractions are 47.9% and 97.0%, respectively.

FACS plot showing cell populations from spleen pre- and post-cell isolation using the EasySep™ Mouse F4/80 Positive Selection Kit

Figure 3. Typical EasySep™ Mouse F4/80 Positive Cell Isolation Profile from Spleen

Starting with naïve mouse splenocytes, the F4/80+ cell content of the isolated fraction is typically 88.8 ± 3.4% (mean ± SD) using the purple EasySep™ magnet. In the above example, the purities of the start and final isolated fractions are 5.4% and 90.6%, respectively.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet - Peritoneal Lavage
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
1000158054 or higher
Language
English
Document Type
Product Information Sheet - Lung Tissue
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
1000158053 or lower
Language
English
Document Type
Product Information Sheet - Spleen
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
1000158054 or higher
Language
English
Document Type
Product Information Sheet - Peritoneal Lavage
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
1000158053 or lower
Language
English
Document Type
Product Information Sheet - Lung Tissue
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
1000158054 or higher
Language
English
Document Type
Product Information Sheet - Spleen
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
1000158053 or lower
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (7)

How to Isolate Mouse Peritoneal Cells
Protocol
How to Isolate Mouse Peritoneal Cells
Frequencies and Percentages of Mouse Immune Cell Types
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
1:23
Video
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
Online Immunology Journal Club: New Strategies to Target Macrophages in Cancer
30:07
Webinar
Online Immunology Journal Club: New Strategies to Target Macrophages in Cancer
Easy Isolation of F4/80-Positive Macrophages from Mouse Tissues
Scientific Poster
Easy Isolation of F4/80-Positive Macrophages from Mouse Tissues
A Serum-Free Medium for Differentiation of Monocytes to Macrophages
Scientific Poster
A Serum-Free Medium for Differentiation of Monocytes to Macrophages

文献 (1)

Novel lipid emulsion for total parenteral nutrition based on 18-carbon n-3 fatty acids elicits a superior immunometabolic phenotype in a murine model compared with standard lipid emulsions. E. Lucchinetti et al. The American journal of clinical nutrition 2022 dec

Abstract

BACKGROUND While lipid emulsions in modern formulations for total parenteral nutrition (TPN) provide essential fatty acids and dense calories, they also promote inflammation and immunometabolic disruptions. OBJECTIVES We aimed to develop a novel lipid emulsion for TPN use with superior immunometabolic actions compared with available standard lipid emulsions. METHODS A novel lipid emulsion [Vegaven (VV)] containing 30% of 18-carbon n-3 fatty acids ($\alpha$-linolenic acid and stearidonic acid) was developed for TPN (VV-TPN) and compared with TPN containing soybean oil-based lipid emulsion (IL-TPN) and fish-oil-based lipid emulsion (OV-TPN). In vivo studies were performed in instrumented male C57BL/6 mice subjected to 7-d TPN prior to analysis of cytokines, indices of whole-body and hepatic glucose metabolism, immune cells, lipid mediators, and mucosal bowel microbiome. RESULTS IL-6 to IL-10 ratios were significantly lower in liver and skeletal muscle of VV-TPN mice when compared with IL-TPN or OV-TPN mice. VV-TPN and OV-TPN each increased hepatic insulin receptor abundance and resulted in similar HOMA-IR values, whereas only VV-TPN increased hepatic insulin receptor substrate 2 and maintained normal hepatic glycogen content, effects that were IL-10-dependent and mediated by glucokinase activation. The percentages of IFN-$\gamma$- and IL-17-expressing CD4+ T cells were increased in livers of VV-TPN mice, and liver macrophages exhibited primed phenotypes when compared with IL-TPN. This immunomodulation was associated with successful elimination of the microinvasive bacterium Akkermansia muciniphila from the bowel mucosa by VV-TPN as opposed to standard lipid emulsions. Assay of hepatic lipid mediators revealed a distinct profile with VV-TPN, including increases in 9(S)-hydroxy-octadecatrienoic acid. When co-administered with IL-TPN, hydroxy-octadecatrienoic acids mimicked the VV-TPN immunometabolic phenotype. CONCLUSIONS We here report the unique anti-inflammatory, insulin-sensitizing, and immunity-enhancing properties of a newly developed lipid emulsion designed for TPN use based on 18-carbon n-3 fatty acids.
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更多信息

更多信息
种属 Mouse
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyPlate™ EasySep™ Magnet (Catalog #18102) • EasyEights™ EasySep™ Magnet (Catalog #18103)
样本来源 Lung, Other, Spleen
Selection Method Positive
标记抗体
  1. 抗小鼠CD11b抗体，克隆M1/70
    抗小鼠CD11b抗体，克隆M1/70

    抗人、小鼠、恒河猴CD11b的大鼠单克隆IgG2b抗体

  2. 抗小鼠CD45抗体，克隆30-F11
    抗小鼠CD45抗体，克隆30-F11

    大鼠单克隆IgG2b抗体，抗小鼠CD45

  3. 抗小鼠F4/80抗体，克隆BM8
    抗小鼠F4/80抗体，克隆BM8

    大鼠单克隆IgG2a抗体，抗小鼠F4/80

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