抗小鼠F4/80抗体，克隆BM8

大鼠单克隆IgG2a抗体，抗小鼠F4/80

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产品号 #（选择产品）

产品号 #60027_C

大鼠单克隆IgG2a抗体，抗小鼠F4/80

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总览

实验数据

产品说明书及文档

应用领域

总览

BM8抗体识别F4/80抗原（在小鼠中也称为Ly-71，在人中也称为EMR-1），这是一种~160 kDa的跨膜糖蛋白，属于G蛋白偶联受体EGF-TM7家族。F4/80被认为是鉴定成熟组织巨噬细胞的首选标志物，在肝脏（Kupffer细胞）、皮肤（Langerhans细胞）、骨髓基质、胰腺、胸腺、脾脏（红髓）、肺等组织中广泛地呈可变表达。循环单核细胞、嗜酸性粒细胞和一部分树突状细胞也表达F4/80。巨噬细胞活化后F4/80表达水平升高。据报导，F4/80是唯一适合区分胰腺中破坏性和非破坏性炎症过程的巨噬细胞标志物。该蛋白被认为在免疫耐受所涉及的CD8+调节性T细胞的产生中发挥作用。

该抗体克隆已被验证可用于评估EasySep™试剂盒（包括EasySep™小鼠单核细胞富集试剂盒（产品号 #19761）和EasySep™小鼠CD11b正选试剂盒（产品号 #18770））分选的细胞纯度。

亚型
一抗

靶抗原
F4/80

别名
EMR1，Ly71

活性物种
小鼠

偶联
Alexa Fluor 488，APC，Biotin 或生物素，FITC，PE，PerCP-Cyanine5.5，未偶联的

宿主物种
大鼠

细胞类型
单核细胞，其它细胞系

种属
小鼠

应用
细胞分选，ELISAs，流式细胞术，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹

研究领域
免疫

克隆
BM8

基因编号
13733

同种型
IgG2a，kappa

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实验数据

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, Alexa Fluor® 488 and anti-mouse CD45 APC.
(B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells processed with the EasySep™ Mouse CD11b Positive Selection Kit and labeled with Anti-Mouse F4/80 Antibody, Clone BM8, Alexa Fluor® 488.
(C) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with a rat IgG2a, kappa Alexa Fluor® 488 isotype control antibody and antimouse CD45 APC.

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, PE and anti-mouse CD45 APC.
(B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with a rat IgG2a, kappa PE isotype control antibody and anti-mouse CD45 APC.

Figure 3. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, followed by a mouse anti-rat IgG2a antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Figure 4. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (Catalog #60019PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE.

Figure 5. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, Biotin, followed by streptavidin (SAV) APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (Catalog #60019PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT), followed by SAV APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE.

Figure 6. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, FITC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (Catalog #60019PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, FITC (Catalog #60076FI) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE.

Figure 7. Data for PerCP-Cy55-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, PerCP-Cy5.5 and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (Catalog #60019PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE.