Tissue processing doesn't need to be complex and time consuming. STEMprep™ takes the hassle out of sample prep with a sleek, customizable interface and built-in temperature control to keep your samples cool and your workflow smooth. Less time prepping, more time generating data that you trust—that’s the power of STEMprep™.
The STEMprep™ Tissue Dissociator enables efficient processing of a wide range of tissues, including mouse brain, liver, lung, spleen, and tumors. In this workflow, tissue samples are placed into a STEMprep™ Sample Tube and processed using the STEMprep™ Tissue Dissociator in combination with the appropriate STEMprep™ Tissue Dissociation Kit. Depending on the selected protocol-dissociation or homogenization-the resulting output is either a single-cell suspension or a sample suitable for nucleic acid isolation. STEMprep™-processed cells are compatible with downstream applications such as cell separation, cell culture, flow cytometry, and other assays.
Figure 2. STEMprep™ Cold Temperature Hold Feature Preserves Mouse Lung and Spleen Cell Viability
Mouse lung and spleen tissues were dissociated using the STEMprep™ Mouse Lung and Spleen Dissociation Kits, respectively. After the protocol run was completed, the samples were held on the STEMprep™ Tissue Dissociator for up to 24 hours at 4°C, 22°C, and 37°C. Viability of total nucleated (A) lung and (B) spleen cells. Data are presented as mean ± SD (n = 3).
Figure 3. RNA Yields from Tissues Homogenized Using the STEMprep™ Tissue Dissociator Are Comparable to Other Common Homogenization Methods.
Mouse (A) brain, (B) lung, or (C) liver tissues were homogenized using the STEMprep™ Tissue Dissociator or an alternative automated system. RNA was subsequently extracted using the EasySep™ Total Nucleic Acid Extraction Kit (with DNase I treatment). The yield of RNA per mg of tissue was assessed using the Qubit Flex Instrument and Qubit RNA Broad Range Assay kit. Data are presented as mean ± SD (n = 8).
Figure 4. STEMprep™ Direct Tissue Homogenization Yields High-Quality RNA
RNA integrity number (RIN) of RNA extracted from mouse (A) brain, (B) lung, or (C) liver tissues. Tissues were homogenized using the STEMprep™ Tissue Dissociator or an alternative automated system. RNA was subsequently extracted from the resulting homogenates by EasySep™ Total Nucleic Acid Extraction Kit (with DNase I treatment). The quality of the extracted RNA was assessed using the 2100 Bioanalyzer Instrument and Agilent RNA 6000 Nano Kit. Data are presented as mean ± SD (n = 8).
Figure 5. STEMprep™ Template Protocol Information
Step 1 (Prime): A brief slow spin during pre-heat.
Steps 2 and 3 (Cut): Steps 2 and 3 are looped with a repeat count of 2. A reverse spin (-40 RPM) is applied to step 3 to ensure free movement of tissue.
Note: Reduce speed for soft tissues. Increase speed or number of loops for hard tissues.
Step 4 (Incubate): Slow spin to agitate tissue during incubation with enzyme cocktail.
Step 5 (Dissociate): High-speed spin to generate single cells.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Microbial dysbiosis sculpts a systemic ILC3/IL-17 axis governing lung inflammatory responses A Kabil et al. Mucosal Immunology 2025 July
Abstract
Advancements in vaccination and sanitation have significantly reduced the prevalence and burden of infectious diseases; however, these benefits have coincided with a marked rise in autoimmune and allergic disorders. Recent studies have investigated these linked trends through the lens of host-microbiome alterations, proposing these shifts as a potential explanatory mechanism. Previously, we demonstrated that vancomycin-induced depletion of short-chain fatty acid (SCFA)-producing bacteria results in hyperactivation of ILC2s and exacerbated allergic responses. Here we investigate the effects of low-dose streptomycin on innate and adaptive immune cell populations and their activation states. Although streptomycin-treated mice exhibit normal allergic responses, they display heightened susceptibility to Th1/Th17-mediated disease, specifically hypersensitivity pneumonitis (HP). This is characterized by a two-fold increase in ILC3s and Th17 cells in the lungs, alongside activation of antigen-presenting cells (APCs) at steady state-an effect that is further amplified upon exposure to HP-inducing agents. Shotgun metagenomic analysis revealed that streptomycin-induced dysbiosis reduces microbial diversity, depletes bile acid-metabolizing bacteria, and enriches for metabolic pathways involved in branched-chain amino acid biosynthesis, including leucine-a known activator of mTORC1. Strikingly, administration of the secondary bile acid metabolite isolithocholic acid (an inverse agonist of RORγt), or an IL-23 neutralizing antibody, reverses the enhanced susceptibility to HP. Inhibition of mTORC1 significantly reduced Th17/ILC3 responses and histopathology. Our findings underscore microbial equilibrium as a key determinant of susceptibility to HP and uncover a positive feedback loop between IL and 23-producing APCs and ILC3/Th17 cells that mechanistically links dysbiosis to sustained type 3 inflammation, and we identify a simple, actionable means of intervention.
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