抗人CD138（Syndecan-1）抗体，克隆 MI15

抗人、恒河猴、食蟹猴CD138（syndecan-1）的小鼠单克隆IgG1抗体

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产品号 #60003_C

抗人、恒河猴、食蟹猴CD138（syndecan-1）的小鼠单克隆IgG1抗体

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总览

实验数据

产品说明书及文档

应用领域

总览

MI15抗体与细胞外表位CD138 （Syndecan-1）反应，CD138 （Syndecan-1）是一种分子量~85 - 95 kDa的1型跨膜糖蛋白，表达于前B细胞、未成熟B细胞、正常和恶性浆细胞（但不表达于成熟循环B细胞）以及非造血细胞（如胚胎间充质细胞、内皮细胞、上皮细胞和神经细胞）的表面。CD138的表达被用作几多种肿瘤的诊断标志物。CD138主要作为一种受体，通过连接细胞外基质和细胞骨架来调节细胞增殖、细胞迁移和细胞-基质关联。附着在CD138上的硫酸肝素和硫酸软骨素部分与多种蛋白质相关联，包括胶原蛋白、纤维连接蛋白、肌腱蛋白、血小板反应蛋白和特定细胞因子。MI15抗体识别与克隆DL-101抗CD138抗体不同的表位，但阻断克隆B-B4的结合。

亚型
一抗

靶抗原
CD138 (Syndecan-1)

别名
B-B4，SDC1，突触黏附素‑1

活性物种
Cynomolgus，人，Rhesus

偶联
APC，FITC，PE，未偶联的

宿主物种
小鼠

细胞类型
B 细胞，血浆

种属
人，非人灵长类

应用
流式细胞术，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹

研究领域
癌症，免疫

克隆
MI15

基因编号
6382

同种型
IgG1，kappa

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实验数据

Figure 1. Data for APC-Conjugated

(A) Flow cytometry analysis of human U266 myeloma cells labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human U266 myeloma cells isolated with EasySep™ Human CD138 Positive Selection Kit from a mixed population of U266 cells and human peripheral blood mononuclear cells. Cells were labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, APC. Histograms show labeling of the starting population containing ~5% U266 cells (Start) and the isolated cells (Isolated). Labeling of the start cells with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (Catalog #60071AZ) is shown (solid line histogram).

Figure 2. Data for FITC-Conjugated

(A) Flow cytometry analysis of human U266 myeloma cells labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human U266 myeloma cells isolated with EasySep™ Human CD138 Positive Selection Kit from a mixed population of U266 cells and human peripheral blood mononuclear cells. Cells were labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, FITC. Histograms show labeling of the starting population containing ~5% U266 cells (Start) and the isolated cells (Isolated). Labeling of the start cells with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60071FI) is shown (solid line histogram).

Figure 3. Data for PE-Conjugated

(A) Flow cytometry analysis of human U266 myeloma cells labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human U266 myeloma cells isolated with EasySep™ Human CD138 Positive Selection Kit from a mixed population of U266 cells and human peripheral blood mononuclear cells. Cells were labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, PE. Histograms show labeling of the starting population containing ~5% U266 cells (Start) and the isolated cells (Isolated). Labeling of the start cells with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (Catalog #60071PE) is shown (solid line histogram).

Figure 4. Data for Unconjugated

Flow cytometry analysis of human U266 myeloma cells labeled with Anti-Human CD138 (Syndecan-1) Antibody, Clone MI15, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI; filled histogram) or a mouse IgG1, kappa isotype control antibody followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (open histogram).