抗人CD2抗体，克隆RPA-2.10

小鼠抗人、恒河猴、食蟹猴CD2的单克隆IgG1抗体

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产品号 #60007_C

小鼠抗人、恒河猴、食蟹猴CD2的单克隆IgG1抗体

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总览

实验数据

产品说明书及文档

应用领域

总览

RPA-2.10抗体可与CD2发生反应。CD2是一种分子量约为50 kDa的 I 型跨膜糖蛋白，属于免疫球蛋白（Ig）超家族；其胞外部分含有两个 Ig 样结构域。CD2广泛表达于外周T细胞、NK细胞、树突状细胞、红细胞、大多数胸腺T细胞、部分胸腺B细胞亚群和内皮细胞。CD2的表达出现在T细胞分化早期。在某些淋巴瘤和髓系白血病中观察到异常表达。CD2对于T细胞的激活和信号传导以及淋巴细胞粘附至关重要。CD2的主要配体是位于抗原呈递细胞上的CD58（LFA-3），其他配体包括CD15（SSEA-1）、CD48和CD59。值得注意的是，RPA-2.10抗体可阻断混合淋巴细胞反应。

亚型
一抗

靶抗原
CD2

别名
LFA-2，SRBC-R，T11

活性物种
Baboon，Capuchin Monkey，Chimpanzee，Cynomolgus，人，Pig，Pigtailed Macaque，Rhesus，Squirrel Monkey

偶联
APC，FITC，PE，PerCP

宿主物种
小鼠

细胞类型
T 细胞

种属
人，非人灵长类，其它细胞系

应用
细胞分选，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，Western印迹

研究领域
免疫

克隆
RPA-2.10

基因编号
914

同种型
IgG1，kappa

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实验数据

Figure 1. Data for Anti-Human CD2 Antibody, Clone RPA-2.10, APC

(A) Flow cytometry analysis of human buffy coat cells labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (black line histogram).
(B) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) processed with the EasySep™ HLA CD3 Positive Selection Kit and labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (open histogram).

Figure 2. Data for Anti-Human CD2 Antibody, Clone RPA-2.10, Biotin

(A) Flow cytometry analysis of human buffy coat cells labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, Biotin followed by streptavidin (SAV) APC (filled histogram) or a mouse IgG1, kappa biotin isotype control antibody followed by SAV APC (black line histogram).
(B) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) processed with the EasySep™ HLA CD3 Positive Selection Kit and labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, Biotin followed by streptavidin (SAV) APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa biotin isotype control antibody followed by SAV APC is shown (open histogram).

Figure 3. Data for Anti-Human CD2 Antibody, Clone RPA-2.10, FITC

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, FITC and anti-human CD45 APC.
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ HLA CD3 Positive Selection Kit and labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, FITC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (open histogram).

Figure 4. Data for Anti-Human CD2 Antibody, Clone RPA-2.10, PE

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, PE and anti-human CD45 APC. (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ HLA CD3 Positive Selection Kit and labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, PE. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa PE isotype control antibody is shown (open histogram).

Figure 5. Data for Anti-Human CD2 Antibody, Clone RPA-2.10

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI; filled histogram), or a mouse IgG1, kappa isotype control antibody followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Image showing flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, PerCP or a mouse IgG1, kappa isotype control antibody, PerCP.

Figure 6. Data for Anti-Human CD2 Antibody, Clone RPA-2.10, PerCP

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD2 Antibody, Clone RPA-2.10, PerCP (filled histogram) or a mouse IgG1, kappa isotype control antibody, PerCP (solid line histogram).