抗人CD71（转铁蛋白受体）抗体，克隆OKT9

小鼠单克隆IgG1抗体，抗人CD71（转铁蛋白受体）

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产品号 #60106_C

小鼠单克隆IgG1抗体，抗人CD71（转铁蛋白受体）

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总览

实验数据

产品说明书及文档

应用领域

总览

OKT9抗体识别CD71（转铁蛋白受体），CD71是一种~180 - 190 kDa的二硫键结合的同型二聚体，属于2型跨膜糖蛋白，在多种活跃增殖的细胞、网织红细胞、单核细胞、巨噬细胞和骨髓基质细胞上高水平表达。CD71在静息白细胞表面的表达水平较低，但在受抗原或丝裂原刺激后的淋巴细胞、单核细胞和巨噬细胞表面CD71表达水平上调。在网织红细胞向成熟红细胞分化的过程中，CD71表达会丢失。CD71通过受体介导的内吞作用和转铁蛋白-受体复合物到细胞表面的再循环来介导转铁蛋白-铁复合物的摄取。CD71已被用作评估红系前体和评估红系白血病和骨髓增生异常综合征等疾病的标志物。CD71也被用作药物递送的靶标。据报导，OKT9抗体的结合表位与抗体克隆CY1G4重叠，但与转铁蛋白的配体结合位点不同。

亚型
一抗

靶抗原
CD71 (Transferrin Receptor)

别名
Mtvr1，p90，T9，TFR，TFRC，TFR1，TR，转铁蛋白受体，TRFR

活性物种
人

偶联
APC，Biotin 或生物素，FITC，PE，未偶联的

宿主物种
小鼠

细胞类型
红系细胞

种属
人

应用
流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，显微镜技术，Western印迹

研究领域
干细胞生物学

克隆
OKT9

基因编号
7037

同种型
IgG1，kappa

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实验数据

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of CD3+ cells (T cells) isolated from human peripheral blood mononuclear cells (PBMCs), then stimulated by incubation with anti-CD3 and anti-CD28 antibodies. Cells were labeled with Anti-Human CD71 (Transferrin Receptor) Antibody, Clone OKT9, followed by a rat anti-mouse IgG1 antibody, PE (filled histogram), or a mouse IgG1, kappa isotype control antibody followed by a rat anti-mouse IgG1 antibody, PE (solid line histogram).
(B) Flow cytometry analysis of CD3+ cells isolated from human PBMCs, then stimulated by incubation with anti-CD3 and anti-CD28 antibodies. Cells were labeled with Anti-Human CD71 (Transferrin Receptor) Antibody, Clone OKT9, which had been pre-incubated for 5 min with a rat anti-mouse IgG1 antibody, PE (filled histogram), or a mouse IgG1, kappa isotype control antibody, which had been pre-incubated for 5 min with a rat anti-mouse IgG1 antibody, PE (solid line histogram). Pre-incubating the primary and secondary antibodies helped minimize background binding of the secondary antibody to the anti-CD3 and anti-CD28 antibodies used for stimulating the cells.

Figure 2. Data for APC-Conjugated

Flow cytometry analysis of CD3+ cells (T cells) isolated from human peripheral blood mononuclear cells (PBMCs), then stimulated by incubation with anti-CD3 and anti-CD28 antibodies. Cells were labeled with Anti-Human CD71 (Transferrin Receptor) Antibody, Clone OKT9, APC (filled histogram) or a mouse IgG1, kappa isotype control antibody, APC (solid line histogram).

Figure 3. Data for Biotin-Conjugated

Flow cytometry analysis of CD3+ cells (T cells) isolated from human peripheral blood mononuclear cells (PBMCs), then stimulated by incubation with anti-CD3 and anti-CD28 antibodies. Cells were labeled with Anti-Human CD71 (Transferrin Receptor) Antibody, Clone OKT9, Biotin followed by streptavidin (SAV) APC (filled histogram), or a biotinylated mouse IgG1, kappa isotype control antibody followed by SAV APC (solid line histogram).

Figure 4. Data for FITC-Conjugated

Flow cytometry analysis of CD3+ cells (T cells) isolated from human peripheral blood mononuclear cells (PBMCs), then stimulated by incubation with anti-CD3 and anti-CD28 antibodies. Cells were labeled with Anti-Human CD71 (Transferrin Receptor) Antibody, Clone OKT9, FITC (filled histogram) or a mouse IgG1, kappa isotype control antibody, FITC (solid line histogram).

Figure 5. Data for PE-Conjugated

Flow cytometry analysis of CD3+ cells (T cells) isolated from human peripheral blood mononuclear cells (PBMCs), then stimulated by incubation with anti-CD3 and anti-CD28 antibodies. Cells were labeled with Anti-Human CD71 (Transferrin Receptor) Antibody, Clone OKT9, PE (filled histogram) or a mouse IgG1, kappa isotype control antibody, PE (solid line histogram).