EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #02692_C
人红细胞扩增的无血清培养补充物
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总览
StemSpan™ 红系扩增添加物（100X）含有多种重组人细胞因子，用于选择性地促进从人脐带血（CB）或骨髓（BM）样本中分离的CD34+细胞向红系祖细胞扩增和分化。当添加到无血清培养基中时，StemSpan™红系扩增添加物通常可在14天的液体培养中促进每个CD34+细胞生成数千个红细胞（以CD34+人CB细胞为起始样本）。请查看数据了解更多详细信息。StemSpan™红系扩增添加物（100X）可与以下任何StemSpan™培养基结合使用：•StemSpan™ SFEM（产品号 #09600）•StemSpan™ SFEM II（产品号 #09605）•StemSpan™-XF（产品号 #100-0073）•StemSpan™-ACF红系扩增培养基（产品号 #09860）
包含
• 重组人干细胞因子（SCF）
• 重组人白介素3（IL-3）
• 重组人促红细胞生成素（EPO）
• 本产品仅含重组蛋白和合成成分
分类
添加剂
细胞类型
红系细胞，造血干/祖细胞
种属
人
应用
细胞培养，分化，扩增
品牌
StemSpan
研究领域
干细胞生物学，移植研究
制剂类别
不含动物成分，无血清
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (11)
文献 (3)
Peripheral blood derived induced pluripotent stem cells (iPSCs) from a female with familial hypertrophic cardiomyopathy. Stem cell research 2017
Abstract
Induced pluripotent stem cells (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) obtained from a 62-year-old female with familial hypertrophic cardiomyopathy (HCM). PBMCs were reprogrammed to a pluripotent state following transfection with non-integrative episomal vectors carrying reprogramming factors OCT4, SOX2, LIN28, KLF4 and L-MYC. iPSCs were shown to express pluripotency markers, possess trilineage differentiation potential, carry rare variants identified in DNA isolated directly from the patient's whole blood, have a normal karyotype and no longer carry episomal vectors for reprogramming. This line is a useful resource for identifying unknown genetic causes of HCM.
Generation of induced pluripotent stem cells (iPSCs) from a hypertrophic cardiomyopathy patient with the pathogenic variant p.Val698Ala in beta-myosin heavy chain (MYH7) gene. Stem cell research 2017
Abstract
Induced pluripotent stem cells (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) isolated from the whole blood of a 43-year-old male with hypertrophic cardiomyopathy (HCM) who carries the pathogenic variant p.Val698Ala in beta-myosin heavy chain (MYH7). Patient-derived PBMCs were reprogrammed using non-integrative episomal vectors containing reprogramming factors OCT4, SOX2, LIN28, KLF4 and L-MYC. iPSCs were shown to express pluripotent markers, have trilineage differentiation potential, carry the pathogenic MYH7 variant p.Val698Ala, have a normal karyotype and no longer carry the episomal reprogramming vector. This line is useful for studying the link between variants in MYH7 and the pathogenesis of HCM.
Selection-free genome editing of the sickle mutation in human adult hematopoietic stem/progenitor cells. Science translational medicine 2016 OCT
Abstract
Genetic diseases of blood cells are prime candidates for treatment through ex vivo gene editing of CD34+ hematopoietic stem/progenitor cells (HSPCs), and a variety of technologies have been proposed to treat these disorders. Sickle cell disease (SCD) is a recessive genetic disorder caused by a single-nucleotide polymorphism in the $\beta$-globin gene (HBB). Sickle hemoglobin damages erythrocytes, causing vasoocclusion, severe pain, progressive organ damage, and premature death. We optimize design and delivery parameters of a ribonucleoprotein (RNP) complex comprising Cas9 protein and unmodified single guide RNA, together with a single-stranded DNA oligonucleotide donor (ssODN), to enable efficient replacement of the SCD mutation in human HSPCs. Corrected HSPCs from SCD patients produced less sickle hemoglobin RNA and protein and correspondingly increased wild-type hemoglobin when differentiated into erythroblasts. When engrafted into immunocompromised mice, ex vivo treated human HSPCs maintain SCD gene edits throughout 16 weeks at a level likely to have clinical benefit. These results demonstrate that an accessible approach combining Cas9 RNP with an ssODN can mediate efficient HSPC genome editing, enables investigator-led exploration of gene editing reagents in primary hematopoietic stem cells, and suggests a path toward the development of new gene editing treatments for SCD and other hematopoietic diseases.
更多信息
| 种属 | Human |
|---|---|
| Contains | • Recombinant human stem cell factor (SCF) • Recombinant human interleukin 3 (IL-3) • Recombinant human erythropoietin (EPO) • This product contains only recombinant proteins and synthetic components |
| 配方类别 | Animal Component-Free, Serum-Free |
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