StemSpan™-ACF红系扩增培养基

用于培养和扩增人红系细胞的无动物成分培养基

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产品号 #（选择产品）

产品号 #09860_C

用于培养和扩增人红系细胞的无动物成分培养基

产品优势

专为红系祖细胞扩增和分化设计的无动物成分培养条件
为搭配使用StemSpan™红系扩增添加物而优化
可从每个起始CD34+细胞产生数千个CD71+GlyA+红系细胞

产品组分包括

专为红系祖细胞扩增和分化设计的无动物成分培养条件
为搭配使用StemSpan™红系扩增添加物而优化
可从每个起始CD34+细胞产生数千个CD71+GlyA+红系细胞

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总览

实验数据

产品说明书及文档

应用领域

总览

StemSpan™-ACF红系扩增培养基（ACF-E）是一种无动物成分（ACF）培养基，用于人造血细胞的体外培养和扩增。当搭配使用StemSpan™红系扩增添加物（100X; 产品号 #02692）时，该培养基可在ACF培养条件下支持人造血祖细胞扩增并分化为CD71+和血型糖蛋白A+（GlyA+）的成红细胞。该培养基仅包含重组蛋白和合成成分，不含血清或其他人或动物来源的成分。

包含
This product contains only recombinant proteins and synthetic components

亚型
专用培养基

细胞类型
红系细胞，造血干/祖细胞

种属
人

应用
细胞培养，分化，扩增

品牌
StemSpan

研究领域
干细胞生物学，移植研究

制剂类别
Animal Component-Free，无血清，Xeno-Free

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实验数据

Figure 1. Production of Erythroid Cells from Human CB-Derived CD34+ Cells Cultured in StemSpan™ Media Containing StemSpan™ Erythroid Expansion Supplement

(A) Average numbers of erythroid cells generated after culturing purified CD34+ CB cells (n = 5) for 14 days in StemSpan™ SFEM (black bars), SFEM II (grey bars), or StemSpan™-ACF Erythroid Expansion Medium (ACF-E, orange bars) media containing StemSpan™ Erythroid Expansion Supplement (Catalog #02692). Shown are the number of erythroid cells that express CD71 and/or Glycophorin A (GlyA) per input CD34+ cell. (B) The percentages of the different erythroid cell subsets generated in these cultures are shown, including CD71+GlyA+ erythroblasts, immature CD71+GlyA- erythroid progenitor cells and pro-erythroblasts, and CD71-/lowGlyA+ normoblasts. All three media supported the generation of thousands of erythroid cells per CB-derived CD34+ cell plated.

Table 1. Production of Erythroid Cells from Human CB-Derived CD34+ Cells Cultured in StemSpan™ Media Containing StemSpan™ Erythroid Expansion Supplement

Yields and percentages of erythroid cells produced after culturing purified CD34+ CB cells (n = 5) for 14 days in StemSpan™ SFEM, SFEM II or ACF-E media containing Erythroid Expansion Supplement. Erythroid cells were identified by flow cytometry after staining with antibodies against CD71 and GlyA. The % erythroid cells represent the percentage of cells that express CD71 and/or GlyA.

*CI: Confidence Interval.