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抗人 TRA-2-49 抗体,克隆 TRA-2-49/6E

抗人、黑猩猩、长臂猿TRA-2-49(组织非特异性碱性磷酸酶)的小鼠单克隆IgG1抗体

产品号 #(选择产品)

产品号 #60066_C

抗人、黑猩猩、长臂猿TRA-2-49(组织非特异性碱性磷酸酶)的小鼠单克隆IgG1抗体

总览

TRA-2-49/6E 抗体可与碱性磷酸酶 (AP) 的组织非特异性同工酶发生反应,该同工酶在未分化的多能干细胞(例如人类和小鼠胚胎干细胞 (ES) 以及诱导性多能干细胞 (iPS) 和胚胎癌 (EC) 细胞)中高水平表达。该 AP 同工酶与肝脏、骨和肾脏细胞表达的 AP 同工酶相同。分化后 AP 水平会降低,因此 TRA-2-49/6E 抗体可用于监测多能干细胞的分化状态。TRA-2-49/6E 抗体不会与其他通过翻译后修饰而不同的 AP 同工酶发生交叉反应,据报道,它无法识别来自牛、狗、山羊、豚鼠、仓鼠、小鼠、大鼠或绵羊,或恒河猴、狒狒和非洲绿猴等旧世界猴的 AP。

亚型
一抗
 
靶抗原
TRA-2-49
 
别名
肝/骨/肾碱性磷酸酶;组织非特异性碱性磷酸酶
 
活性物种
Cat,人,非人灵长类,Pig,兔
 
偶联
未偶联的
 
宿主物种
小鼠
 
细胞类型
多能干细胞
 
种属
人,非人灵长类,其它细胞系
 
应用
流式细胞术,免疫细胞化学,免疫荧光,免疫组化,免疫沉淀,Western印迹
 
研究领域
干细胞生物学
 
克隆
TRA-2-49/6E
 
基因编号
249
 
同种型
IgG1,kappa
 

实验数据

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of human ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control; dashed line histogram) labeled with AntiHuman TRA-2-49 Antibody, Clone TRA-2-49/6E, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI). Labeling of human ES cells with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC is shown (solid line histogram). (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and labeled with Anti-Human TRA-2-49 Antibody, Clone TRA-2-49/6E, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with a mouse IgG1, kappa isotype control antibody, followed by goat anti-mouse IgG, FITC. (C) Flow cytometry analysis of human iPS cells labeled with Anti-Human TRA-2-49 Antibody, Clone TRA-2-49/6E, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram). (D) Western blot analysis of denatured/reduced cell lysates from human ES cells (lane 1) or HT1080 fibrosarcoma cells (negative control, lane 2) with Anti-Human TRA-2-49 Antibody, Clone TRA-2-49/6E.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60066
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60066
Lot #
All
Language
English

应用领域

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更多信息

更多信息
种属 Human, Non-Human Primate, Other
克隆 TRA-2-49/6E
Gene Id 249
Alternative Names Liver/bone/kidney alkaline phosphatase, tissue non-specific alkaline phosphatase
同种型/isotype IgG1, kappa
法律声明:

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

质量保证:

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