产品号 #60067_C
抗人、黑猩猩、长臂猿TRA-2-54(组织非特异性碱性磷酸酶)的小鼠单克隆IgG1抗体
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TRA-2-54/2J抗体与碱性磷酸酶(AP)的组织非特异性异构体反应,AP在未分化的多能干细胞,如人类和小鼠胚胎干细胞(ES),以及诱导多能干细胞(iPS)和胚胎癌(EC)细胞中高水平表达。AP的同工酶与肝、骨和肾细胞表达的同工酶相同。AP水平随着分化而降低,因此,TRA-2-54/2J抗体可用于监测多能干细胞的分化状态。TRA-2-54/2J抗体不与其他AP同工酶发生交叉反应,这些酶因翻译后修饰而不同,据报道,该抗体不识别来自牛、狗、山羊、豚鼠、仓鼠、小鼠、大鼠或羊的AP,也不识别恒河猴、狒狒和非洲绿猴等旧大陆猴子。
亚型
一抗
靶抗原
TRA-2-54
别名
肝/骨/肾碱性磷酸酶;组织非特异性碱性磷酸酶
活性物种
Cat,Chimpanzee,人,非人灵长类,Pig,兔
偶联
未偶联的
宿主物种
小鼠
细胞类型
多能干细胞
种属
人,非人灵长类,其它细胞系
应用
流式细胞术,免疫细胞化学,免疫荧光,免疫组化,Western印迹
研究领域
干细胞生物学
克隆
TRA-2-54/2J
基因编号
249
同种型
IgG1,kappa
Figure 1. Data for Unconjugated
(A) Flow cytometry analysis of human ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control; dashed line histogram) labeled with Anti-Human TRA-2-54 Antibody, Clone TRA-2-54/2J, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI). Labeling of human ES cells with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by Goat Anti-Mouse IgG (H+L), Polyclonal, FITC is shown (solid line histogram). (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and labeled with Anti-Human TRA-2-54 Antibody, Clone TRA-2-54/2J, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with a mouse IgG1, kappa isotype control antibody, followed by goat anti-mouse IgG, FITC. (C) Flow cytometry analysis of human iPS cells labeled with Anti-Human TRA-2-54 Antibody, Clone TRA-2-54/2J, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram). (D) Western blot analysis of denatured/reduced cell lysates from human ES cells (lane 1) or HT1080 fibrosarcoma cells (negative control, lane 2) with Anti-Human TRA-2-54 Antibody, Clone TRA-2-54/2J.
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种属 | Human, Non-Human Primate, Other |
---|---|
克隆 | TRA-2-54/2J |
Gene Id | 249 |
Alternative Names | Liver/bone/kidney alkaline phosphatase, tissue non-specific alkaline phosphatase |
同种型/isotype | IgG1, kappa |
人多能干细胞系,冷冻
小鼠单克隆IgG1, kappa同型对照抗体
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