抗小鼠CD150抗体，克隆TC15-12F12.2

抗小鼠CD150（SLAM）大鼠单克隆IgG2a抗体

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产品号 #（选择产品）

产品号 #60036_C

抗小鼠CD150（SLAM）大鼠单克隆IgG2a抗体

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总览

实验数据

产品说明书及文档

应用领域

总览

TC15-12F12.2抗体与CD150（信号淋巴细胞活化分子，SLAM）发生反应。CD150是一种分子量约为75 kDa的I型跨膜糖蛋白，作为细胞粘附分子和/或辅助受体，在免疫反应中发挥多种作用。它在T细胞、未成熟胸腺细胞、B细胞、树突状细胞、巨噬细胞和内皮细胞中存在差异表达。其表达模式因细胞类型和活化状态而异。T细胞、B细胞和树突状细胞活化后，CD150会快速诱导表达，且T细胞的合成主要在Th1细胞克隆上进行，而非Th2细胞克隆。CD150介导的TCR激活T细胞的共刺激作用可增强Th1细胞产生IFN-γ，而这种应答可通过TC15-12F12.2抗体的结合得到增强。 CD150被认为通过与细胞内蛋白酪氨酸磷酸酶SHP-2结合来介导信号转导。CD150还在造血细胞发育中发挥作用，并且是检测多能造血干细胞的有效标记物（与CD48和CD41等其他标记物协同作用）。CD150在非多能造血祖细胞上不表达。

亚型
一抗

靶抗原
CD150 (SLAM)

别名
IPO-3，信号淋巴细胞活化分子

活性物种
小鼠

偶联
Alexa Fluor 488，APC，Biotin 或生物素，PE，PE-Cyanine7，未偶联的

宿主物种
Hamster，大鼠

细胞类型
造血干/祖细胞

种属
小鼠

应用
流式细胞术，功能学筛选，免疫荧光，免疫组化，免疫沉淀

研究领域
干细胞生物学

克隆
TC15-12F12.2

基因编号
27218

同种型
IgG2a，lambda

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实验数据

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD150 Antibody, Clone TC15-12F12.2, Alexa Fluor® 488 (filled histogram) or a rat IgG2a, lambda Alexa Fluor® 488 isotype control antibody (solid line histogram).

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD150 Antibody, Clone TC15-12F12.2, PE (filled histogram) or a rat IgG2a, lambda PE isotype control antibody (solid line histogram). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD150 Antibody, Clone TC15-12F12.2, PE (filled histogram) or a rat IgG2a, lambda PE isotype control antibody (solid line histogram).

Figure 3. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD150 Antibody, Clone TC15-12F12.2, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or a rat IgG2a isotype control antibody followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD150 Antibody, Clone TC15-12F12.2, APC (filled histogram) or a rat IgG2a isotype control antibody, APC (solid line histogram).

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD150 Antibody, Clone TC15-12F12.2, Biotin followed by streptavidin (SAV) APC (filled histogram), or a biotinylated rat IgG2a isotype control antibody followed by SAV APC (solid line histogram).

(A) Primary human airway epithelial cells were cultured in PneumaCult™-ALI Medium (Catalog #05001) at the air-liquid interface, then cryo-sectioned and labeled with Anti-Human MUC1 (CD227) Antibody, Clone 16A, followed by a goat anti-rabbit IgG antibody, Alexa Fluor® 594 (red), and an anti-human NGF Receptor/p75NTR (CD271) antibody, followed by a donkey anti-mouse IgG antibody, Alexa Fluor® 488 (green). (B) Flow cytometry analysis of human peripheral blood lymphocytes following stimulation with PHA for 3 days. Cells were labeled with Anti-Human MUC1 (CD227) Antibody, Clone 16A, followed by an anti-mouse IgG1 antibody, PE and anti-human CD3 antibody, Clone HIT3a, Pacific Blue™. (C) Flow cytometry analysis of human peripheral blood lymphocytes following stimulation with PHA for 3 days. Cells were labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by an anti-mouse IgG1 antibody, PE, and anti-human CD3 antibody, clone HIT3a, Pacific Blue™.

(A) Flow cytometry analysis of human airway epithelial cells cultured in PneumaCult™-ALI Medium (Catalog #05001) at the air-liquid interface. Cells were enzymatically dissociated and labeled with Anti-Human MUC1 (CD227) Antibody, Clone 16A, PE (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (Catalog #60070PE, solid line histogram). (C) Flow cytometry analysis human peripheral blood lymphocytes following stimulation with PHA for 3 days. Cells were labeled with Anti-Human MUC1 (CD227) Antibody, Clone 16A, PE and anti-human CD3 antibody, clone HIT3a, Pacific Blue™. (D) Flow cytometry analysis of PHA-activated human peripheral blood lymphocytes following stimulation with PHA for 3 days. Cells were labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE, and anti-human CD3 antibody, clone HIT3a, Pacific Blue™.

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD150 Antibody, Clone TC15-12F12.2, PE-Cyanine7 (filled histogram) or a rat IgG2a isotype control antibody, PE-Cyanine7 (solid line histogram).