抗小鼠CD48（SLAMF2）抗体，克隆HM48-1

仓鼠（亚美尼亚）抗小鼠 CD48（SLAMF2）单克隆 IgG1 抗体

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产品号 #60162_C

仓鼠（亚美尼亚）抗小鼠 CD48（SLAMF2）单克隆 IgG1 抗体

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总览

实验数据

产品说明书及文档

应用领域

总览

HM48-1抗体可与小鼠CD48发生反应。CD48是一种45 kDa GPI锚定糖蛋白，属于信号淋巴细胞活化分子(SLAM)家族和免疫球蛋白超家族。SLAM家族成员在免疫功能的多个方面发挥作用，包括共刺激、细胞毒性和细胞因子产生。CD48还在细胞粘附和T细胞活化中发挥作用。在小鼠中，CD48被认为存在于除中性粒细胞和ESLAM细胞（具有长期自我更新能力的CD45+EPCR+CD48-CD150+造血干细胞）以外的几乎所有造血细胞中。CD48的配体包括CD2和CD244，它们的结合有助于促进免疫细胞之间的相互作用。据报道，HM48-1能够在体外和体内阻断CD48:CD2和CD48:CD244的相互作用。此外，HM48-1 还能抑制脾细胞对有丝分裂原的增殖反应、抑制 CTL 活性并提高心脏移植的存活率。

亚型
一抗

靶抗原
CD48 (SLAMF2)

活性物种
小鼠

偶联
APC

宿主物种
Hamster

细胞类型
B 细胞，造血干/祖细胞，巨噬细胞，单核细胞，T 细胞

应用
流式细胞术

研究领域
免疫，干细胞生物学

克隆
HM48-1

基因编号
12506

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实验数据

(A) Primary human airway epithelial cells were cultured in PneumaCult™-ALI Medium (Catalog #05001) at the air-liquid interface, then cryo-sectioned and labeled with Anti-Human MUC1 (CD227) Antibody, Clone 16A, followed by a goat anti-rabbit IgG antibody, Alexa Fluor® 594 (red), and an anti-human NGF Receptor/p75NTR (CD271) antibody, followed by a donkey anti-mouse IgG antibody, Alexa Fluor® 488 (green). (B) Flow cytometry analysis of human peripheral blood lymphocytes following stimulation with PHA for 3 days. Cells were labeled with Anti-Human MUC1 (CD227) Antibody, Clone 16A, followed by an anti-mouse IgG1 antibody, PE and anti-human CD3 antibody, Clone HIT3a, Pacific Blue™. (C) Flow cytometry analysis of human peripheral blood lymphocytes following stimulation with PHA for 3 days. Cells were labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by an anti-mouse IgG1 antibody, PE, and anti-human CD3 antibody, clone HIT3a, Pacific Blue™.

(A) Flow cytometry analysis of human airway epithelial cells cultured in PneumaCult™-ALI Medium (Catalog #05001) at the air-liquid interface. Cells were enzymatically dissociated and labeled with Anti-Human MUC1 (CD227) Antibody, Clone 16A, PE (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (Catalog #60070PE, solid line histogram). (C) Flow cytometry analysis human peripheral blood lymphocytes following stimulation with PHA for 3 days. Cells were labeled with Anti-Human MUC1 (CD227) Antibody, Clone 16A, PE and anti-human CD3 antibody, clone HIT3a, Pacific Blue™. (D) Flow cytometry analysis of PHA-activated human peripheral blood lymphocytes following stimulation with PHA for 3 days. Cells were labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE, and anti-human CD3 antibody, clone HIT3a, Pacific Blue™.

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD48 (SLAMF2) Antibody, Clone HM48-1, APC (filled histogram) or an Armenian hamster IgG isotype control antibody, APC (solid line histogram).