Optimized Workflows for High-Efficiency Genome Editing in Stem and Primary Cell Types
CRISPR-Cas genome editing in cell culture systems is a powerful technique for disease modeling and the development of cellular therapies. Compared to work with immortalized cell lines, genome editing of stem and primary cells presents unique challenges, including issues related to efficient delivery and expression of CRISPR machinery, clonogenicity, and cytotoxicity. In this webinar, our in-house expert, Dr. Ashley Watson, discusses optimized workflows for CRISPR-Cas9 genome editing in human pluripotent stem cells and primary T cells.
ClonaCell™ EasyPick: Automated High Throughput Cell Line Generation
STEMCELL Technologies automated ClonaCell™ EasyPick Platform merges the rapid selection and cloning of cells using ClonaCell™ media with Hamilton's 30 years of experience in robotics to meet the needs of cell line development for biologics production
Nature Research Round Table: Maintenance of Human Pluripotent Stem Cells In Vitro
Dr. Tenneille Ludwig from the WiCell Research Institute provides information on in vitro culture systems for ES and iPS cell maintenance. The WiCell Research Institute is widely known for their human pluripotent stem cell (hPSC) banking services, with over 1,350 cell lines available to researchers around the globe. This presentation and the following Q&A session were moderated by Dr. Ludovic Vallier from the Wellcome Trust Sanger Institute.
This presentation was part of a Round Table series titled “Challenges in Ensuring hPSC Quality”, hosted in partnership with Nature Research. Global experts gathered at the Springer Nature headquarters in London, UK, to tackle some of the most pertinent issues impacting the use of human pluripotent stem cells (hPSCs), ranging from fundamental biology research to therapeutic applications. Explore the full series here.
Nature Research Round Table: Genomic Integrity of Human Pluripotent Stem Cells
Dr. Martin Pera from the Jackson Laboratory and International Stem Cell Initiative (ISCI) discusses the biological effects of recurrent genetic variants that arise in human pluripotent stem cells (hPSCs) in culture and during differentiation. This presentation and the following Q&A session were moderated by Dr. Peter Andrews from the University of Sheffield.
This presentation was part of a Round Table series titled “Challenges in Ensuring hPSC Quality”, hosted in partnership with Nature Research. Global experts gathered at the Springer Nature headquarters in London, UK, to tackle some of the most pertinent issues impacting the use of human pluripotent stem cells (hPSCs), ranging from fundamental biology research to therapeutic applications. Explore the full series here.
Note: Some original data from this presentation has been omitted to abide by copyright rules.
Nature Research Round Table: Genome Editing in Human Pluripotent Stem Cells
Dr. Ludovic Vallier from the Wellcome Trust Sanger Institute discusses considerations for genome editing in human pluripotent stem cells (hPSCs). Dr. Vallier is involved at the Cambridge Biomedical Research Center Core Facility, whose services include providing genome-edited iPS cell lines for disease modeling and developing approaches for cell-based therapies. This presentation and the following Q&A session were moderated by Dr. Alex Alderton from the Wellcome Trust Sanger Institute.
This presentation was part of a Round Table series titled “Challenges in Ensuring hPSC Quality”, hosted in partnership with Nature Research. Global experts gathered at the Springer Nature headquarters in London, UK, to tackle some of the most pertinent issues impacting the use of human pluripotent stem cells (hPSCs), ranging from fundamental biology research to therapeutic applications. Explore the full series here.
Note: Some original data from this presentation has been omitted to abide by copyright rules.
Development of Simplified and Defined Cell Culture for Human Pluripotent Stem Cells
Human pluripotent stem cells (hPSCs) have the ability to generate all cell types in the human body and can be used in many applications in basic research and translational medicine, including disease modeling, drug screening and cell therapy. Maintenance of high quality hPSCs is dependent on consistent in vitro cell culture conditions and handling techniques. TeSR™-E8™ is a defined, xeno-free medium with a simple and published formula that was developed to meet the demands of a wide range of applications. The E8 culture system has been successfully used to derive and maintain hPSCs, and enzyme-free methods have also been developed for cell expansion and cryopreservation. More recently, defined differentiation conditions have also been established for specific lineages. These developments will provide an efficient and cost-effective E8 platform for the hPSC field. This webinar is presented by Dr. Guokai Chen at the University of Macau.
EasySep™小鼠TIL（CD45）正选试剂盒
Optimized Workflows for High-Efficiency Genome Editing in Stem and Primary Cell Types CRISPR-Cas genome editing in cell culture systems is a powerful technique for disease modeling and the development of cellular therapies. Compared to work with immortalized cell lines, genome editing of stem and primary cells presents unique challenges, including issues related to efficient delivery and expression of CRISPR machinery, clonogenicity, and cytotoxicity. In this webinar, our in-house expert, Dr. Ashley Watson, discusses optimized workflows for CRISPR-Cas9 genome editing in human pluripotent stem cells and primary T cells.
How to Generate Neural Progenitor Cells from hPSCs Using STEMdiff™ Neural Induction Medium STEMdiff™ Neural Induction Medium is a defined, serum-free and highly efficient medium for the differentiation of hESCs & iPSCs to NPCs
ClonaCell™ EasyPick: Automated High Throughput Cell Line Generation STEMCELL Technologies automated ClonaCell™ EasyPick Platform merges the rapid selection and cloning of cells using ClonaCell™ media with Hamilton's 30 years of experience in robotics to meet the needs of cell line development for biologics production
Nature Research Round Table: Maintenance of Human Pluripotent Stem Cells In Vitro Dr. Tenneille Ludwig from the WiCell Research Institute provides information on in vitro culture systems for ES and iPS cell maintenance. The WiCell Research Institute is widely known for their human pluripotent stem cell (hPSC) banking services, with over 1,350 cell lines available to researchers around the globe. This presentation and the following Q&A session were moderated by Dr. Ludovic Vallier from the Wellcome Trust Sanger Institute.
Nature Research Round Table: Genomic Integrity of Human Pluripotent Stem Cells Dr. Martin Pera from the Jackson Laboratory and International Stem Cell Initiative (ISCI) discusses the biological effects of recurrent genetic variants that arise in human pluripotent stem cells (hPSCs) in culture and during differentiation. This presentation and the following Q&A session were moderated by Dr. Peter Andrews from the University of Sheffield.
Nature Research Round Table: Genome Editing in Human Pluripotent Stem Cells Dr. Ludovic Vallier from the Wellcome Trust Sanger Institute discusses considerations for genome editing in human pluripotent stem cells (hPSCs). Dr. Vallier is involved at the Cambridge Biomedical Research Center Core Facility, whose services include providing genome-edited iPS cell lines for disease modeling and developing approaches for cell-based therapies. This presentation and the following Q&A session were moderated by Dr. Alex Alderton from the Wellcome Trust Sanger Institute.
How to Reprogram Fibroblasts into Human Induced Pluripotent Stem (iPS) Cells Using ReproRNA™-OKSGM How to reprogram fibroblasts in using ReproRNA™-OKSGM is a non-integrating, self-replicating RNA reprogramming vector for generating human induced pluripotent stem (iPS) cells
Development of Simplified and Defined Cell Culture for Human Pluripotent Stem Cells Human pluripotent stem cells (hPSCs) have the ability to generate all cell types in the human body and can be used in many applications in basic research and translational medicine, including disease modeling, drug screening and cell therapy. Maintenance of high quality hPSCs is dependent on consistent in vitro cell culture conditions and handling techniques. TeSR™-E8™ is a defined, xeno-free medium with a simple and published formula that was developed to meet the demands of a wide range of applications. The E8 culture system has been successfully used to derive and maintain hPSCs, and enzyme-free methods have also been developed for cell expansion and cryopreservation. More recently, defined differentiation conditions have also been established for specific lineages. These developments will provide an efficient and cost-effective E8 platform for the hPSC field. This webinar is presented by Dr. Guokai Chen at the University of Macau.
