EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #19656_C
全血免疫磁珠负选试剂盒
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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
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EasySep™ BufferCell separation buffer
总览
EasySep™ Direct人嗜酸性粒细胞分选试剂盒，通过免疫磁性负选，直接从人全血中分选出具有功能的、高纯度的嗜酸性粒细胞。无需裂解、密度梯度离心或其他处理步骤，分选得到的细胞可立即用于流式细胞术、功能分析、细胞培养及其他后续应用。
磁体兼容性
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
亚型
细胞分选试剂盒
细胞类型
粒细胞及其亚群
种属
人
样本来源
Whole Blood
筛选方法
Negative
应用
细胞分选
品牌
EasySep
研究领域
免疫
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (11)
文献 (1)
Neutrophils from severe asthmatic patients induce epithelial to mesenchymal transition in healthy bronchial epithelial cells. Respiratory research 2019 oct
Abstract
BACKGROUND Asthma is a heterogenous disease characterized by chronic inflammation and airway remodeling. An increase in the severity of airway remodeling is associated with a more severe form of asthma. There is increasing interest in the epithelial to mesenchymal transition process and mechanisms involved in the differentiation and repair of the airway epithelium, especially as they apply to severe asthma. Growing evidence suggests that Epithelial-Mesenchymal transition (EMT) could contribute to airway remodeling and fibrosis in asthma. Severe asthmatic patients with remodeled airways have a neutrophil driven inflammation. Neutrophils are an important source of TGF-$\beta$1, which plays a role in recruitment and activation of inflammatory cells, extracellular matrix (ECM) production and fibrosis development, and is a potent inducer of EMT. OBJECTIVE As there is little data examining the contribution of neutrophils and/or their mediators to the induction of EMT in airway epithelial cells, the objective of this study was to better understand the potential role of neutrophils in severe asthma in regards to EMT. METHODS We used an in vitro system to investigate the neutrophil-epithelial cell interaction. We obtained peripheral blood neutrophils from severe asthmatic patients and control subjects and examined for their ability to induce EMT in primary airway epithelial cells. RESULTS Our data indicate that neutrophils from severe asthmatic patients induce changes in morphology and EMT marker expression in bronchial epithelial cells consistent with the EMT process when co-cultured. TGF-$\beta$1 levels in the culture medium of severe asthmatic patients were increased compared to that from co-cultures of non-asthmatic neutrophils and epithelial cells. CONCLUSIONS AND CLINICAL RELEVANCE As an inducer of EMT and an important source of TGF-$\beta$1, neutrophils may play a significant role in the development of airway remodeling and fibrosis in severe asthmatic airways.
更多信息
| 种属 | Human |
|---|---|
| Magnet Compatibility | • “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) • EasyEights™ EasySep™ Magnet (Catalog #18103) |
| 样本来源 | Whole Blood |
| Selection Method | Negative |
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标记抗体
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抗人CD45抗体，克隆HI30小鼠单克隆IgG1抗体，抗人、黑猩猩CD45
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抗人CD66b抗体，克隆G10F5小鼠单克隆IgM抗体，抗人、黑猩猩CD66b
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抗人CD16抗体，克隆号3G8小鼠抗人、恒河猴、食蟹猴CD16单克隆IgG1抗体
