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EasySep™小鼠CD90.1正选试剂盒

免疫磁珠正选细胞分选试剂盒
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产品号 #（选择产品）

产品号 #18958_C

免疫磁珠正选细胞分选试剂盒

产品优势

  • 快速、易于操作
  • 纯度高达97%
  • 无需分离柱
  • 分选得到的细胞无荧光标记

产品组分包括

  • EasySep™小鼠CD90.1正选试剂盒（产品号 #18958）
    • EasySep™小鼠CD90.1正选抗体混合物，1mL
    • EasySep™ Dextran RapidSpheres™ 50100 磁珠，2 X 1mL
  • EasySep™小鼠CD138正选试剂盒（产品号 #18957RF）
    • EasySep™小鼠CD90.1正选抗体混合物，1mL
    • EasySep™ Dextran RapidSpheres™ 50100 磁珠，2 X 1mL
    • RoboSep™ 缓冲液（产品号 #20104）
    • RoboSep™过滤吸头（产品 号#20125）
专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. EasyEights™EasySep™磁铁
    EasyEights™EasySep™磁铁
  2. EasySep™缓冲液
    EasySep™缓冲液
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

EasySep™ 小鼠CD90.1正选试剂盒专为从脾细胞、淋巴结及外周血的单细胞悬液中通过正选分离CD90.1+（Thy1.1+）细胞而设计。目的细胞被识别CD14的四聚体抗体复合物和磁珠标记。通过EasySep™磁极分离被标记的目的细胞，无需使用分离柱。目的细胞被保留在分离管中，而非目的细胞倒出即可。

磁极兼容性
• EasySep™磁极（产品号 #18000） • “The Big Easy” EasySep™磁极（产品号 #18001） • EasyPlate™ EasySep™磁极（产品号 #18102） • EasyEights™ EasySep™磁极（产品号 #18103） • RoboSep™-S（产品号 #21000）
 
分类
细胞分选试剂盒
 
细胞类型
T 细胞
 
种属
小鼠
 
样本来源
其它细胞系，脾脏
 
分选方法
正选
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫
 

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实验数据

FACS Histogram Results with EasySep™ Mouse CD90.1 Positive Selection Kit

Figure 1. FACS Histogram Results with EasySep™ Mouse CD90.1 Positive Selection Kit

Starting with mouse splenocytes, the CD90.1+ (Thy1.1+) cell content of the isolated fraction is typically 93.5 ± 3.9% (mean ± SD; using the purple EasySep™ Magnet).

FACS Histogram Results with EasySep™ Mouse CD90.1 Positive Selection Kit on Adoptive Lymphocyte Transfer Samples

Figure 2. FACS Histogram Results with EasySep™ Mouse CD90.1 Positive Selection Kit on Adoptive Lymphocyte Transfer Samples

Starting with mouse splenocytes from adoptive lymphocyte transfer samples, the CD90.1+ (Thy1.1+) cell content of the isolated fraction is typically 90.8 ± 1.5% (mean ± SD; using the purple EasySep™ Magnet).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™ Mouse CD90.1 Positive Selection Kit
Catalog #
18958RF
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ Mouse CD90.1 Positive Selection Kit
Catalog #
18958
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Mouse CD90.1 Positive Selection Kit
Catalog #
18958RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Mouse CD90.1 Positive Selection Kit
Catalog #
18958RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Mouse CD90.1 Positive Selection Kit
Catalog #
18958RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Mouse CD90.1 Positive Selection Kit
Catalog #
18958
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Mouse CD90.1 Positive Selection Kit
Catalog #
18958
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (9)

Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Mouse Cell Isolation
Brochure
Mouse Cell Isolation
EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Frequencies and Percentages of Mouse Immune Cell Types
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
0:57
Video
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
Using Stable Isotope Tracing to Study CD8+ Immune Cell Dynamics In Vivo
34:58
Webinar
Using Stable Isotope Tracing to Study CD8+ Immune Cell Dynamics In Vivo

常见问题

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

更多信息

更多信息
种属 Mouse
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyPlate™ EasySep™ Magnet (Catalog #18102) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™- S (Catalog #21000)
样本来源 Other, Spleen
Selection Method Positive
标记抗体
  1. 抗大鼠CD90抗体，克隆OX-7
    抗大鼠CD90抗体，克隆OX-7

    小鼠单克隆IgG1抗体，抗小鼠、大鼠、豚鼠CD90/CD90.1

质量保证:

产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
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