FreSR™- S

用于将胚胎干细胞和诱导多能干细胞以单细胞形式冻存的无动物成分冻存液

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产品号 #（选择产品）

产品号 #05859_C

用于将胚胎干细胞和诱导多能干细胞以单细胞形式冻存的无动物成分冻存液

产品优势

成分明确、无血清、无动物成分的冻存液，用于将ESC/iPSC以单细胞形式冻存
ESC/iPSC复苏后可迅速恢复细胞集落
可重复的高回收率
针对在TeSR™维持培养基中培养的ESC/iPSC的冻存进行了优化
保持ESC/iPSC的多能性和扩增能力
便捷、即用型

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总览

FreSR™-S是一种成分确定、无血清、无动物成分的冻存液，用于将人多能干细胞（hPSCs）以单细胞形式进行冻存。这款完全即用型冻存液推荐用于使用mTeSR™ 1、mTeSR™ Plus、TeSR™ 2或TeSR™-E8™中培养的hPSCs。冻存的hPSCs应在-135°C（液氮）或更低的温度下储存。

实验数据

Figure 1. High Viability and Recovery of Cells Stored in FreSR™-S

hPSCs cryopreserved as single cells using FreSR™-S have (A) higher post-thaw recovery (number of cells recovered / number of cells frozen) and (B) maintain higher viability (number of live cells / total number of cells) compared to competitor medium. All data values are plotted as percentages, (n=18, p < 0.0001 for each).

Figure 2. More Vials Frozen and Banked With FreSR™-S

hPSCs are cryopreserved in FreSR™-S at lower cell density compared to traditional methods. Graph indicates the number of vials cryopreserved for each well of a 6 well plate that is harvested.
Note: 1 vial is typically thawed and seeded directly into 1 well of a six well plate. Cells should be cultured as aggregates after thawing.

Figure 3. hPSCs Frozen and Thawed as Single Cells with FreSR™-S Display a Normal Karyotype

Karyograms of (A-B) WLS-4D1 hiPS cells and (C-D) H1 hES cells that were frozen and thawed as single cells using FreSR™-S. (A, C) Thawed cells were seeded into culture containing TeSR™-E8™ medium and 10 µM Y-27632 and maintained as aggregates for five passages. (B,D) hPSCs were also subjected to a second freeze-thaw cycle as single cells with FreSR™-S and cultured for five passages as aggregates prior to collection of karyotype data.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type

Product Name

Catalog #

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Language

Document Type

Product Information Sheet

Product Name

FreSR™-S

Catalog #

05859

Lot #

All

Language

English

Document Type

Safety Data Sheet

Product Name

FreSR™-S

Catalog #

05859

Lot #

All

Language

English

应用领域

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文献 (1)

Abstract

We have observed karyotypic changes involving the gain of chromosome 17q in three independent human embryonic stem (hES) cell lines on five independent occasions. A gain of chromosome 12 was seen occasionally. This implies that increased dosage of chromosome 17q and 12 gene(s) provides a selective advantage for the propagation of undifferentiated hES cells. These observations are instructive for the future application of hES cells in transplantation therapies in which the use of aneuploid cells could be detrimental.

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种属	Human
配方类别	Animal Component-Free, Serum-Free

FreSR™- S

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产品号 #05859_C

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FreSR™- S

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产品号 #05859_C

产品优势

总览

实验数据

产品说明书及文档

应用领域

相关材料与文献

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