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人IL-1 β酶联免疫吸附测定试剂盒

用于人白细胞介素1 β的检测和测量

产品号 #(选择产品)

产品号 #02004_C

用于人白细胞介素1 β的检测和测量

产品组分包括

  • 人IL-1 β酶联免疫吸附测定试剂盒,2板(目录#02004)
    • 人IL-1β酶联免疫吸附测定板,2
    • 人IL-1β标准品,1瓶
    • Human IL-1β Biotinylated Detection Antibody, 50µL
    • Sa-hrp, 30µl
    • 标准重组缓冲液B, 1ml
    • 洗涤缓冲液(20X), 120 mL
    • ELISA稀释液,120 mL
    • SA-HRP稀释液,25ml
    • TMB底物,25ml
    • 停止液,25ml
    • 粘接板盖,6个
  • 人IL-1 β酶联免疫吸附测定试剂盒,10板(目录#02005)
    • 人IL-1β ELISA板,10x
    • 人IL-1β标准品,1瓶
    • Human IL-1β Biotinylated Detection Antibody, 250µL
    • Sa-hrp, 130µl
    • 标准重组缓冲液B, 1ml
    • 洗涤缓冲液(20倍),5 × 120毫升
    • ELISA稀释剂,3 × 120ml
    • SA-HRP稀释剂,120 mL
    • TMB底物,120 mL
    • 停止液,120ml
    • 胶粘剂板盖,30x

总览

人白细胞介素1β(IL-1β)ELISA试剂盒用于定量检测和测定血清、血浆和细胞培养上清液等生物体液中的人IL-1β。IL-1β由单核细胞、巨噬细胞和树突状细胞分泌。IL-1β是一种促炎细胞因子,它通过刺激粘附分子的表达,将免疫活性细胞募集到受累部位。

该检测基于夹心ELISA方法,将样品加入预先包被有针对该细胞因子的特异性捕获抗体的ELISA板中。通过添加生物素化的检测抗体,然后加入与生物素化抗体结合的链霉亲和素-辣根过氧化物酶,即可检测捕获的细胞因子。添加显色酶底物3,3',5,5' 四甲基联苯胺(TMB)会产生有色产物,其强度与样品中细胞因子的浓度成正比。通过与平行分析的细胞因子标准的连续稀释度进行比较来确定细胞因子的浓度。

分类
完整试剂盒
 
细胞类型
树突状细胞(DCs),巨噬细胞,单核细胞
 
种属

 
研究领域
免疫
 

实验数据

Representative Standard Curve

• Reportable Range: 1 - 316 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 4 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC international standard 86/680.
• Recovery: A mid-curve recovery of 83 - 98% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 2.6% (CV). The inter-assay precision of this assay is 3.6% (CV).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

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应用领域

本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。

相关材料与文献

技术资料 (2)

更多信息

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种属 Human
质量保证:

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