产品号 #05590_C
用于naïve态人胚胎干细胞和iPS细胞扩增的细胞培养基
若您需要咨询产品或有任何技术问题,请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系
用于naïve态人胚胎干细胞和iPS细胞扩增的细胞培养基
Coating for cultureware
Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer
RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2
Compatible antibodies for purity assessment of isolated cells
使用成分明确的细胞培养基,可高效扩增naïve态人类胚胎干细胞(ES)和诱导多能干细胞(iPS)。在该培养基中培养naïve态人多能干细胞(hPSCs)具有折射边缘的均匀圆顶、相位明亮的集落,并保持与重置Naive hPSC相当的基因表达谱(参考文献:Guo et al. Stem Cell Reports, 2016; Guo et al. Development, 2017; Takashima et al. Cell, 2014)。
在重置为始发态(re-priming)后,这些细胞可实现三胚层方向的分化,具备向所有三种体细胞谱系分化的能力。
该培养基基于Austin Smith的t2iL + Gö配方,并获得英国剑桥大学剑桥干细胞研究所的授权,其兼容广泛,适用于使用NaïveCult™诱导试剂盒(产品号:#05580)获得的重置naïve人ES/iPS细胞、转基因诱导的重置型hPSC(Takashima 等,2014)以及胚胎来源的重置型hPSC(Guo 等,2016)
在重置为始发态(re-priming)后,这些细胞可实现三胚层方向的分化,具备向所有三种体细胞谱系分化的能力。
该培养基基于Austin Smith的t2iL + Gö配方,并获得英国剑桥大学剑桥干细胞研究所的授权,其兼容广泛,适用于使用NaïveCult™诱导试剂盒(产品号:#05580)获得的重置naïve人ES/iPS细胞、转基因诱导的重置型hPSC(Takashima 等,2014)以及胚胎来源的重置型hPSC(Guo 等,2016)
在重置为始发态(re-priming)后,这些细胞可实现三胚层方向的分化,具备向所有三种体细胞谱系分化的能力。
该培养基基于Austin Smith的t2iL + Gö配方,并获得英国剑桥大学剑桥干细胞研究所的授权,其兼容广泛,适用于使用NaïveCult™诱导试剂盒(产品号:#05580)获得的重置naïve人ES/iPS细胞、转基因诱导的重置型hPSC(Takashima 等,2014)以及胚胎来源的重置型hPSC(Guo 等,2016)
在重置为始发态(re-priming)后,这些细胞可实现三胚层方向的分化,具备向所有三种体细胞谱系分化的能力。
该培养基基于Austin Smith的t2iL + Gö配方,并获得英国剑桥大学剑桥干细胞研究所的授权,其兼容广泛,适用于使用NaïveCult™诱导试剂盒(产品号:#05580)获得的重置naïve人ES/iPS细胞、转基因诱导的重置型hPSC(Takashima 等,2014)以及胚胎来源的重置型hPSC(Guo 等,2016)
亚型
专用培养基
细胞类型
多能干细胞
种属
人
应用
细胞培养,扩增,培养
品牌
NaïveCult
研究领域
干细胞生物学
Figure 1. Schematic of Reversion of Primed to Naïve hPSCs
Primed hPSCs, grown either in mTeSR™1 or TeSR™-E8™ on Corning® Matrigel®, are plated as single cells on inactivated murine embryonic fibroblasts (iMEFs) and treated with Rho-kinase inhibition (10 μM Y-27632) for 24 hours in hypoxic conditions. On day 1, medium is changed to Induction Medium 1 supplemented with a histone deacetylase inhibitor (HDACi) and cells are cultured for 3 days with daily medium changes. On day 4, medium is changed to Induction Medium 2 and cells are cultured until day 11 with daily medium changes. On day 11, hPSCs are passaged as single cells onto fresh iMEFs and subsequently passaged every 3 - 4 days until the end of passage 2 in Induction Medium 2. From passage 3, hPSCs are cultured in Induction Medium 3. Background differentiation will decrease between passage 3 and 8. At this time cells can be transferred into NaïveCult™ Expansion Medium for long-term maintenance and expansion.
Figure 2. Human ES and iPS Cells Can Be Reverted to a Naïve State
Representative images of human (A) passage-7 H9 ES cells and (B) passage-9 WLS-1C iPS cells that were reverted to a naïve state using the NaïveCult™ Induction Kit and subsequently cultured in NaïveCult™ Expansion Medium. During reversion, colonies change from a flat morphology to a tightly packed, domed morphology with refractive edges characteristic of naïve-state hPSCs.
Figure 3. hPSCs Cultured in the NaïveCult™ Media System Express High Levels of Factors Associated with Naïve hPSCs
Gene expression profile of human (A) H9 ES cells and (B) WLS-1C iPS cells reverted using the NaïveCult™ Induction Kit and maintained in NaïveCult™ Expansion Medium.
Figure 4. Reset Naïve Human PSCs Cultured in NaïveCult™ Expansion Medium are Capable of Tri-Lineage Differentiation Following Re-Priming
Reset naïve human iPS cells cultured in NaïveCult™ Expansion Medium were re-primed in mTeSR™1 and differentiated to all three somatic lineages. (A) Representative flow cytometry plot of human WLS-1C iPS cells differentiated using the STEMdiff™ Definitive Endoderm Kit (Catalog #05110) demonstrating >90% CXCR4+/SOX17+ definitive endodermal progenitors. (B) Representative flow cytometry plot of human WLS-1C iPS cells differentiated using STEMdiff™ Mesodermal Induction Medium (Catalog #05220) demonstrating >80% Brachyury+/OCT4- mesodermal progenitors. (C) Representative immunofluorescence image of PAX6 (green) and SOX1 (red) double positive neural progenitors derived from human WLS-1C iPS cells differentiated using the STEMdiff™ SMADi Neural Induction Kit (Catalog #08581).
请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。
本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。
Thank you for your interest in IntestiCult™ Organoid Growth Medium (Human). Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
种属 | Human |
---|
无转基因诱导人ES细胞和iPS细胞重置为原始状态的成分明确型培养基试剂盒
无饲养层、无动物成分的人胚胎干细胞和诱导多能干细胞维持培养基
cGMP标准、无饲养层的人胚胎干细胞(ES)和诱导多能干细胞(iPS)维持培养基
扫描二维码或搜索微信号STEMCELLTech,即可关注我们的微信平台,第一时间接收丰富的技术资源和最新的活动信息。
如您有任何问题,欢迎发消息给STEMCELLTech微信公众平台,或与我们通过电话/邮件联系:400 885 9050 INFO.CN@STEMCELL.COM。