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NaïveCult™扩增培养基

用于naïve态人胚胎干细胞和iPS细胞扩增的细胞培养基

产品号 #(选择产品)

产品号 #05590_C

用于naïve态人胚胎干细胞和iPS细胞扩增的细胞培养基

产品优势

  • 支持naïve态人ES和iPS细胞的稳健生长;
  • 维持PSC 的naïve态相关的基因高表达;
  • 与不依赖转基因的、转基因诱导的和胚胎来源的重置naïve人类ES和iPS细胞兼容;
  • 成分明确,采用高品质、预筛选原材料配制而成

产品组分包括

  • NaïveCult™扩增基础培养基,400 mL;
  • NaïveCult™5X扩增添加物,100mL;
  • NaïveCult™1000X扩增添加物,500µL
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总览

使用成分明确的细胞培养基,可高效扩增naïve态人类胚胎干细胞(ES)和诱导多能干细胞(iPS)。在该培养基中培养naïve态人多能干细胞(hPSCs)具有折射边缘的均匀圆顶、相位明亮的集落,并保持与重置Naive hPSC相当的基因表达谱(参考文献:Guo et al. Stem Cell Reports, 2016; Guo et al. Development, 2017; Takashima et al. Cell, 2014)。

在重置为始发态(re-priming)后,这些细胞可实现三胚层方向的分化,具备向所有三种体细胞谱系分化的能力。

该培养基基于Austin Smith的t2iL + Gö配方,并获得英国剑桥大学剑桥干细胞研究所的授权,其兼容广泛,适用于使用NaïveCult™诱导试剂盒(产品号:#05580)获得的重置naïve人ES/iPS细胞、转基因诱导的重置型hPSC(Takashima 等,2014)以及胚胎来源的重置型hPSC(Guo 等,2016)

在重置为始发态(re-priming)后,这些细胞可实现三胚层方向的分化,具备向所有三种体细胞谱系分化的能力。

该培养基基于Austin Smith的t2iL + Gö配方,并获得英国剑桥大学剑桥干细胞研究所的授权,其兼容广泛,适用于使用NaïveCult™诱导试剂盒(产品号:#05580)获得的重置naïve人ES/iPS细胞、转基因诱导的重置型hPSC(Takashima 等,2014)以及胚胎来源的重置型hPSC(Guo 等,2016)

在重置为始发态(re-priming)后,这些细胞可实现三胚层方向的分化,具备向所有三种体细胞谱系分化的能力。

该培养基基于Austin Smith的t2iL + Gö配方,并获得英国剑桥大学剑桥干细胞研究所的授权,其兼容广泛,适用于使用NaïveCult™诱导试剂盒(产品号:#05580)获得的重置naïve人ES/iPS细胞、转基因诱导的重置型hPSC(Takashima 等,2014)以及胚胎来源的重置型hPSC(Guo 等,2016)

在重置为始发态(re-priming)后,这些细胞可实现三胚层方向的分化,具备向所有三种体细胞谱系分化的能力。

该培养基基于Austin Smith的t2iL + Gö配方,并获得英国剑桥大学剑桥干细胞研究所的授权,其兼容广泛,适用于使用NaïveCult™诱导试剂盒(产品号:#05580)获得的重置naïve人ES/iPS细胞、转基因诱导的重置型hPSC(Takashima 等,2014)以及胚胎来源的重置型hPSC(Guo 等,2016)

亚型
专用培养基
 
细胞类型
多能干细胞
 
种属

 
应用
细胞培养,扩增,培养
 
品牌
NaïveCult
 
研究领域
干细胞生物学
 

实验数据

Figure 1. Schematic of Reversion of Primed to Naïve hPSCs

Primed hPSCs, grown either in mTeSR™1 or TeSR™-E8™ on Corning® Matrigel®, are plated as single cells on inactivated murine embryonic fibroblasts (iMEFs) and treated with Rho-kinase inhibition (10 μM Y-27632) for 24 hours in hypoxic conditions. On day 1, medium is changed to Induction Medium 1 supplemented with a histone deacetylase inhibitor (HDACi) and cells are cultured for 3 days with daily medium changes. On day 4, medium is changed to Induction Medium 2 and cells are cultured until day 11 with daily medium changes. On day 11, hPSCs are passaged as single cells onto fresh iMEFs and subsequently passaged every 3 - 4 days until the end of passage 2 in Induction Medium 2. From passage 3, hPSCs are cultured in Induction Medium 3. Background differentiation will decrease between passage 3 and 8. At this time cells can be transferred into NaïveCult™ Expansion Medium for long-term maintenance and expansion.

Figure 2. Human ES and iPS Cells Can Be Reverted to a Naïve State

Representative images of human (A) passage-7 H9 ES cells and (B) passage-9 WLS-1C iPS cells that were reverted to a naïve state using the NaïveCult™ Induction Kit and subsequently cultured in NaïveCult™ Expansion Medium. During reversion, colonies change from a flat morphology to a tightly packed, domed morphology with refractive edges characteristic of naïve-state hPSCs.

Figure 3. hPSCs Cultured in the NaïveCult™ Media System Express High Levels of Factors Associated with Naïve hPSCs

Gene expression profile of human (A) H9 ES cells and (B) WLS-1C iPS cells reverted using the NaïveCult™ Induction Kit and maintained in NaïveCult™ Expansion Medium.

Figure 4. Reset Naïve Human PSCs Cultured in NaïveCult™ Expansion Medium are Capable of Tri-Lineage Differentiation Following Re-Priming

Reset naïve human iPS cells cultured in NaïveCult™ Expansion Medium were re-primed in mTeSR™1 and differentiated to all three somatic lineages. (A) Representative flow cytometry plot of human WLS-1C iPS cells differentiated using the STEMdiff™ Definitive Endoderm Kit (Catalog #05110) demonstrating >90% CXCR4+/SOX17+ definitive endodermal progenitors. (B) Representative flow cytometry plot of human WLS-1C iPS cells differentiated using STEMdiff™ Mesodermal Induction Medium (Catalog #05220) demonstrating >80% Brachyury+/OCT4- mesodermal progenitors. (C) Representative immunofluorescence image of PAX6 (green) and SOX1 (red) double positive neural progenitors derived from human WLS-1C iPS cells differentiated using the STEMdiff™ SMADi Neural Induction Kit (Catalog #08581).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

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应用领域

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种属 Human
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