切换导航
  • 比较产品
我的购物车

若您需要咨询产品或有任何技术问题，请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系

EasySep™ HLA嵌合体全血CD33正选试剂盒

免疫磁珠正选人CD33+髓系细胞
率先评论此产品
添加并比较

产品号 #（选择产品）

产品号 #17885_C

免疫磁珠正选人CD33+髓系细胞

产品优势

  • 操作简单、快速
  • 纯度高达99%
  • 无需分离柱

产品组分包括

  • EasySep™ HLA嵌合全血CD3正选试剂盒（产品号#17885）
    • EasySep™ HLA嵌合全血CD3正选抗体混合物，3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101 磁珠，3 x 1 mL
    • EasySep™ 10X浓缩红细胞裂解试剂，10 mL（产品号#20110）    
  • RoboSep™ HLA嵌合全血CD33阳性分选试剂盒（含过滤枪头）（产品号#17885RF）
    • EasySep™ HLA嵌合全血CD3正选抗体混合物，3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101 磁珠，3 x 1 mL
    • EasySep™ 10X浓缩红细胞裂解试剂，10 mL（产品号#20110）    
    • RoboSep™ 缓冲液（产品号 #20104）
    • RoboSep™过滤枪头（产品号 #20125）
main product photo
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. The Big Easy EasySep™ Magnet
    "The Big Easy" EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

总览
实验数据
产品说明书及文档
应用领域
相关材料与文献

总览

使用EasySep™ HLA嵌合全血CD33正选试剂盒，通过免疫磁珠正选技术，从新鲜人全血或白膜层样本中分离高纯度人CD33+髓系细胞。EasySep™技术结合单克隆抗体的特异性和无柱磁珠系统的简便性，已在发表的研究中广泛应用超过20年。

在此EasySep™正选流程中，目的细胞通过被CD33的抗体复合物及磁珠结合标记，并通过 EasySep™ 磁极进行无柱分选，只需倾倒非目标细胞即可，目标细胞则保留在管中。分选后的细胞可立即用于下游应用，例如流式 、细胞培养、或DNA/RNA提取以用于谱系特异性嵌合分析。CD33抗原在髓系祖细胞、粒细胞和单核细胞表面表达。

本产品取代EasySep™人全血CD33阳性分选试剂盒（产品号#18287）和EasySep™ HLA全血CD33阳性分选试剂盒（产品号#18287HLA），可实现更快速的细胞分离。

深入了解免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现全自动化的免疫磁珠细胞分选，以节省时间并提升实验室通量。探索更多为您的实验流程优化的产品，包括细胞表征、冷存等相关产品。

在此EasySep™正选流程中，目的细胞通过被CD33的抗体复合物及磁珠结合标记，并通过 EasySep™ 磁极进行无柱分选，只需倾倒非目标细胞即可，目标细胞则保留在管中。分选后的细胞可立即用于下游应用，例如流式 、细胞培养、或DNA/RNA提取以用于谱系特异性嵌合分析。CD33抗原在髓系祖细胞、粒细胞和单核细胞表面表达。

本产品取代EasySep™人全血CD33阳性分选试剂盒（产品号#18287）和EasySep™ HLA全血CD33阳性分选试剂盒（产品号#18287HLA），可实现更快速的细胞分离。

深入了解免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现全自动化的免疫磁珠细胞分选，以节省时间并提升实验室通量。探索更多为您的实验流程优化的产品，包括细胞表征、冷存等相关产品。

在此EasySep™正选流程中，目的细胞通过被CD33的抗体复合物及磁珠结合标记，并通过 EasySep™ 磁极进行无柱分选，只需倾倒非目标细胞即可，目标细胞则保留在管中。分选后的细胞可立即用于下游应用，例如流式 、细胞培养、或DNA/RNA提取以用于谱系特异性嵌合分析。CD33抗原在髓系祖细胞、粒细胞和单核细胞表面表达。

本产品取代EasySep™人全血CD33阳性分选试剂盒（产品号#18287）和EasySep™ HLA全血CD33阳性分选试剂盒（产品号#18287HLA），可实现更快速的细胞分离。

深入了解免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现全自动化的免疫磁珠细胞分选，以节省时间并提升实验室通量。探索更多为您的实验流程优化的产品，包括细胞表征、冷存等相关产品。

在此EasySep™正选流程中，目的细胞通过被CD33的抗体复合物及磁珠结合标记，并通过 EasySep™ 磁极进行无柱分选，只需倾倒非目标细胞即可，目标细胞则保留在管中。分选后的细胞可立即用于下游应用，例如流式 、细胞培养、或DNA/RNA提取以用于谱系特异性嵌合分析。CD33抗原在髓系祖细胞、粒细胞和单核细胞表面表达。

本产品取代EasySep™人全血CD33阳性分选试剂盒（产品号#18287）和EasySep™ HLA全血CD33阳性分选试剂盒（产品号#18287HLA），可实现更快速的细胞分离。

深入了解免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现全自动化的免疫磁珠细胞分选，以节省时间并提升实验室通量。探索更多为您的实验流程优化的产品，包括细胞表征、冷存等相关产品。

磁体兼容性
• “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
 
亚型
细胞分选试剂盒
 
细胞类型
粒细胞及其亚群，髓系细胞
 
种属
人
 
样本来源
Buffy Coat，Whole Blood
 
筛选方法
Positive
 
应用
细胞分选
 
品牌
EasySep
 
研究领域
嵌合体，HLA，免疫
 

查看更多 显示更少

实验数据

Typical EasySep™ HLA Chimerism Whole Blood CD33 Positive Selection Profile

Figure 1. Typical EasySep™ HLA Chimerism Whole Blood CD33 Positive Selection Profile

Starting with fresh whole blood, the CD33+ cell content of the isolated fraction is typically 96 ± 2% (mean ± SD) using “The Big Easy” EasySep™ Magnet. In the above example, the purities of the start (as assessed by labeling with anti-CD33) and the final isolated fractions are 68.0% and 96.2% (as assessed by labeling with anti-CD14 and anti-CD66b), respectively.
NOTE: RBCs were removed from the start sample by lysis prior to flow cytometry.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™ HLA Chimerism Whole Blood CD33 Positive Selection Kit
Catalog #
17885RF
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ HLA Chimerism Whole Blood CD33 Positive Selection Kit
Catalog #
17885
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ HLA Chimerism Whole Blood CD33 Positive Selection Kit
Catalog #
17885RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ HLA Chimerism Whole Blood CD33 Positive Selection Kit
Catalog #
17885RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ HLA Chimerism Whole Blood CD33 Positive Selection Kit
Catalog #
17885RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ HLA Chimerism Whole Blood CD33 Positive Selection Kit
Catalog #
17885RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ HLA Chimerism Whole Blood CD33 Positive Selection Kit
Catalog #
17885
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ HLA Chimerism Whole Blood CD33 Positive Selection Kit
Catalog #
17885
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ HLA Chimerism Whole Blood CD33 Positive Selection Kit
Catalog #
17885
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (11)

Cell Separation Products for HLA Analysis
Brochure
Cell Separation Products for HLA Analysis
EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Technical Bulletin
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Fully Automated, High-Purity Cell Isolation for Chimerism Labs
Technical Bulletin
Fully Automated, High-Purity Cell Isolation for Chimerism Labs
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
How to Prepare a Buffy Coat from Whole Blood
3:46
Video
How to Prepare a Buffy Coat from Whole Blood
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
1:41
Video
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™
56:02
Webinar
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™
Isolation of Very Pure Lymphoid (CD3+, CD19+ or CD56+) and Myeloid (CD15+ or CD33/CD66b+) Cell Subsets in as Little as 15 Minutes for Use in Lineage-Specific Chimerism Analysis
Scientific Poster
Isolation of Very Pure Lymphoid (CD3+, CD19+ or CD56+) and Myeloid (CD15+ or CD33/CD66b+) Cell Subse...

文献 (1)

Antibody-Mediated LILRB2-Receptor Antagonism Induces Human Myeloid-Derived Suppressor Cells to Kill Mycobacterium tuberculosis. V. K. Singh et al. Frontiers in immunology 2022

Abstract

Tuberculosis is a leading cause of death in mankind due to infectious agents, and Mycobacterium tuberculosis (Mtb) infects and survives in macrophages (MФs). Although MФs are a major niche, myeloid-derived suppressor cells (MDSCs) are an alternative site for pathogen persistence. Both MФs and MDSCs express varying levels of leukocyte immunoglobulin-like receptor B (LILRB), which regulate the myeloid cell suppressive function. Herein, we demonstrate that antagonism of LILRB2 by a monoclonal antibody (mab) induced a switch of human MDSCs towards an M1-macrophage phenotype, increasing the killing of intracellular Mtb. Mab-mediated antagonism of LILRB2 alone and its combination with a pharmacological blockade of SHP1/2 phosphatase increased proinflammatory cytokine responses and phosphorylation of ERK1/2, p38 MAPK, and NF-kB in Mtb-infected MDSCs. LILRB2 antagonism also upregulated anti-mycobacterial iNOS gene expression and an increase in both nitric oxide and reactive oxygen species synthesis. Because genes associated with the anti-mycobacterial function of M1-MФs were enhanced in MDSCs following mab treatment, we propose that LILRB2 antagonism reprograms MDSCs from an immunosuppressive state towards a pro-inflammatory phenotype that kills Mtb. LILRB2 is therefore a novel therapeutic target for eradicating Mtb in MDSCs.
View publication
View All Publications

更多信息

更多信息
种属 Human
Magnet Compatibility • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
样本来源 Buffy Coat, Whole Blood
Selection Method Positive
标记抗体
  1. 抗人CD14抗体，克隆MoP9
    抗人CD14抗体，克隆MoP9

    抗人、恒河猴、食蟹猴CD14的小鼠单克隆IgG2b抗体

  2. 抗人CD66b抗体，克隆G10F5
    抗人CD66b抗体，克隆G10F5

    小鼠单克隆IgM抗体，抗人、黑猩猩CD66b

  3. 山羊抗小鼠 IgG (H+L) 抗体，多克隆
    山羊抗小鼠 IgG (H+L) 抗体，多克隆

    抗小鼠IgG（H+L）山羊多克隆抗体

  4. 抗人CD14抗体，克隆M5E2
    抗人CD14抗体，克隆M5E2

    抗人、恒河猴、食蟹猴CD14的小鼠单克隆IgG2a抗体

质量保证:

产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
Copyright © 2025 by STEMCELL Technologies. All rights reserved.