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EasySep™小鼠CD8+ T细胞分选试剂盒

通过免疫磁珠负选分离无磁珠标记的小鼠CD8+ T细胞
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产品号 #（选择产品）

产品号 #19853_C

通过免疫磁珠负选分离无磁珠标记的小鼠CD8+ T细胞

产品优势

  • 快速且易于使用
  • 纯度高达95%
  • 不需要列
  • 未接触的活细胞

产品组分包括

  • EasySep™小鼠CD8+ T细胞分选试剂盒（产品号 #19853）
    • EasySep™小鼠CD8+ T细胞分选抗体混合物，0.5 mL
    • EasySep™ Streptavidin RapidSpheres™ 50001磁珠，2 x 1 mL
    • EasySep™小鼠FcR阻断剂，0.2 mL
  • RoboSep™小鼠CD8+ T细胞分选试剂盒（产品号 #19853RF）
    • EasySep™小鼠CD8+ T细胞分选抗体混合物，0.5 mL
    • EasySep™ Streptavidin RapidSpheres™ 50001磁珠，2 x 1 mL
    • EasySep™小鼠FcR阻断剂，0.2 mL
    • RoboSep™ 缓冲液（产品号 #20104）
    • RoboSep™过滤吸头（产品号20125）
main product photo
New format, same high quality! You may notice that your kit contents and packaging look slightly different from previous orders. We are currently updating the format of select EasySep™ Mouse kits to include a Mouse FcR blocker instead of Normal Rat Serum. With this change, all components will now be shipped in a single package, while providing the same cell isolation performance as before.
专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. EasyEights™ EasySep™ Magnet
    EasyEights™ EasySep™ Magnet

    Multiple sample processing magnet for column-free immunomagnetic cell separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

使用EasySep™小鼠CD8+ T细胞分选试剂盒，可通过免疫磁珠负选技术，轻松高效地从脾细胞或其他组织的单细胞悬液中分离高纯度的小鼠CD8+ T细胞。EasySep™无柱免疫磁珠分选技术结合单克隆抗体的特异性和无需分选柱的简便性，20多年来被广泛引用于已发表的文献中。

在此EasySep™负选流程中，非目标细胞会被抗体复合物和磁珠标记。表达以下标志物的非目标细胞将被特异性去除：CD11b、CD45R、Ter119、CD4、CD49b、CD19、CD11c、TCRγδ和CD24。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的细胞分离，接着只需将目的细胞倾倒或吸取至一个新的试管中，仅需17.5分钟即可获得高纯度的CD8+ T细胞，且可立即用于流式细胞术、细胞培养及细胞实验等下游应用。

该产品可替代EasySep™人CD8+ T细胞富集试剂盒 (产品号 #19053) 以进行更快的细胞分选。

深入了解EasySep™免疫磁珠分选技术原理，或探索如何通过RoboSep™实现免疫磁珠细胞分选全自动化。探索为您的实验流程优化的更多产品，包括培养基、添加剂、抗体等。

在此EasySep™负选流程中，非目标细胞会被抗体复合物和磁珠标记。表达以下标志物的非目标细胞将被特异性去除：CD11b、CD45R、Ter119、CD4、CD49b、CD19、CD11c、TCRγδ和CD24。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的细胞分离，接着只需将目的细胞倾倒或吸取至一个新的试管中，仅需17.5分钟即可获得高纯度的CD8+ T细胞，且可立即用于流式细胞术、细胞培养及细胞实验等下游应用。

该产品可替代EasySep™人CD8+ T细胞富集试剂盒 (产品号 #19053) 以进行更快的细胞分选。

深入了解EasySep™免疫磁珠分选技术原理，或探索如何通过RoboSep™实现免疫磁珠细胞分选全自动化。探索为您的实验流程优化的更多产品，包括培养基、添加剂、抗体等。

在此EasySep™负选流程中，非目标细胞会被抗体复合物和磁珠标记。表达以下标志物的非目标细胞将被特异性去除：CD11b、CD45R、Ter119、CD4、CD49b、CD19、CD11c、TCRγδ和CD24。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的细胞分离，接着只需将目的细胞倾倒或吸取至一个新的试管中，仅需17.5分钟即可获得高纯度的CD8+ T细胞，且可立即用于流式细胞术、细胞培养及细胞实验等下游应用。

该产品可替代EasySep™人CD8+ T细胞富集试剂盒 (产品号 #19053) 以进行更快的细胞分选。

深入了解EasySep™免疫磁珠分选技术原理，或探索如何通过RoboSep™实现免疫磁珠细胞分选全自动化。探索为您的实验流程优化的更多产品，包括培养基、添加剂、抗体等。

在此EasySep™负选流程中，非目标细胞会被抗体复合物和磁珠标记。表达以下标志物的非目标细胞将被特异性去除：CD11b、CD45R、Ter119、CD4、CD49b、CD19、CD11c、TCRγδ和CD24。通过EasySep™磁极将被磁珠标记的细胞与未被标记的目的细胞分离，接着只需将目的细胞倾倒或吸取至一个新的试管中，仅需17.5分钟即可获得高纯度的CD8+ T细胞，且可立即用于流式细胞术、细胞培养及细胞实验等下游应用。

该产品可替代EasySep™人CD8+ T细胞富集试剂盒 (产品号 #19053) 以进行更快的细胞分选。

深入了解EasySep™免疫磁珠分选技术原理，或探索如何通过RoboSep™实现免疫磁珠细胞分选全自动化。探索为您的实验流程优化的更多产品，包括培养基、添加剂、抗体等。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyPlate™ EasySep™ Magnet (Catalog 18102) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
 
亚型
细胞分选试剂盒
 
细胞类型
T 细胞，T 细胞，CD8+
 
种属
小鼠
 
样本来源
其它细胞系，Spleen
 
筛选方法
Negative
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫
 

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实验数据

Typical EasySep™ Mouse CD8+ T Cell Isolation Profile

Figure 1. Typical EasySep™ Mouse CD8+ T Cell Isolation Profile

Starting with mouse splenocytes, the CD8+ T cell content (CD3+CD8+) of the isolated fraction is 94.4 ± 0.7% (mean ± SD), using the purple EasySep™ Magnet.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™ Mouse CD8+ T Cell Isolation Kit
Catalog #
19853RF
Lot #
1000120417 or higher
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ Mouse CD8+ T Cell Isolation Kit
Catalog #
19853
Lot #
1000120417 or higher
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Mouse CD8+ T Cell Isolation Kit
Catalog #
19853RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Mouse CD8+ T Cell Isolation Kit
Catalog #
19853RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Mouse CD8+ T Cell Isolation Kit
Catalog #
19853RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ Mouse CD8+ T Cell Isolation Kit
Catalog #
19853RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
RoboSep™ Mouse CD8+ T Cell Isolation Kit
Catalog #
19853RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Mouse CD8+ T Cell Isolation Kit
Catalog #
19853
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Mouse CD8+ T Cell Isolation Kit
Catalog #
19853
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Mouse CD8+ T Cell Isolation Kit
Catalog #
19853
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Mouse CD8+ T Cell Isolation Kit
Catalog #
19853
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (14)

Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Mouse Cell Isolation
Brochure
Mouse Cell Isolation
Cell Enrichment Prior to Cell Sorting
Brochure
Cell Enrichment Prior to Cell Sorting
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Antigen Processing and Presentation
Wallchart
Antigen Processing and Presentation
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
0:57
Video
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
Systemic Dysfunction and Plasticity of the Immune Macroenvironment in Cancer Models
44:55
Webinar
Systemic Dysfunction and Plasticity of the Immune Macroenvironment in Cancer Models
Using Stable Isotope Tracing to Study CD8+ Immune Cell Dynamics In Vivo
34:58
Webinar
Using Stable Isotope Tracing to Study CD8+ Immune Cell Dynamics In Vivo
Peptide-Assisted Genome Editing of Primary Cells
48:59
Webinar
Peptide-Assisted Genome Editing of Primary Cells
Streamline Your Mouse T Cell Research from Isolation to Expansion
30:18
Webinar
Streamline Your Mouse T Cell Research from Isolation to Expansion
T Cell Differentiation and Cancer Immunity
48:32
Webinar
T Cell Differentiation and Cancer Immunity

常见问题

Can EasySep™ Streptavidin RapidSpheres™ be used for either positive or negative selection?

Currently, EasySep™ Streptavidin RapidSphere™ kits are only available for negative selection and work by targeting and removing unwanted cells.

How does the separation work?

Streptavidin RapidSphere™ magnetic particles are crosslinked to unwanted cells using biotinylated antibodies. When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a new tube.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ Streptavidin RapidSphere™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Are cells isolated using EasySep™ RapidSphere™ products FACS-compatible?

Yes. Desired cells are unlabeled and ready to use in downstream applications, such as FACS analysis.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

文献 (53)

Novel immunomodulatory properties of adenosine analogs promote their antiviral activity against SARS-CoV-2. G. Monticone et al. EMBO reports 2024 aug

Abstract

The COVID-19 pandemic reminded us of the urgent need for new antivirals to control emerging infectious diseases and potential future pandemics. Immunotherapy has revolutionized oncology and could complement the use of antivirals, but its application to infectious diseases remains largely unexplored. Nucleoside analogs are a class of agents widely used as antiviral and anti-neoplastic drugs. Their antiviral activity is generally based on interference with viral nucleic acid replication or transcription. Based on our previous work and computer modeling, we hypothesize that antiviral adenosine analogs, like remdesivir, have previously unrecognized immunomodulatory properties which contribute to their therapeutic activity. In the case of remdesivir, we here show that these properties are due to its metabolite, GS-441524, acting as an Adenosine A2A Receptor antagonist. Our findings support a new rationale for the design of next-generation antiviral agents with dual - immunomodulatory and intrinsic - antiviral properties. These compounds could represent game-changing therapies to control emerging viral diseases and future pandemics.
View publication
Liraglutide enhances the effect of checkpoint blockade in lung and liver cancers through the inhibition of neutrophil extracellular traps. D. Chen et al. FEBS open bio 2024 aug

Abstract

Glucagon-like peptide-1 (GLP-1) regulates glycemic excursions by augmenting insulin production and inhibiting glucagon secretion. Liraglutide, a long-acting GLP-1 analog, can improve glycemic control for treating type 2 diabetes and prevent neutrophil extravasation in inflammation. Here, we explored the role of liraglutide in the development and therapy of murine lung and liver cancers. In this study, liraglutide substantially decreased circulating neutrophil extracellular trap (NET) markers myeloperoxidase, elastase, and dsDNA in Lewis lung cancer (LLC) and Hepa1-6 tumor-bearing mice. Furthermore, liraglutide downregulated NETs and reactive oxygen species (ROS) of neutrophils in the tumor microenvironment. Functionally, in??vitro experiments showed that liraglutide reduced NET formation by inhibiting ROS. In addition, we showed that liraglutide enhanced the anti-tumoral efficiency of programmed cell death-1 (PD-1) inhibition in LLC and Hepa1-6 tumor-bearing C57BL/6 mice. However, the removal of NETs significantly weakened the antitumor efficiency of liraglutide. We further demonstrated that the long-term antitumor CD8+ T cell responses induced by the combination therapy rejected rechallenges by respective tumor cell lines. Taken together, our findings suggest that liraglutide may promote the anti-tumoral efficiency of PD-1 inhibition by reducing NETs in lung and liver cancers.
View publication
S100a9 Protects Against the Effects of Repeated Social Defeat Stress. C. M. Moshfegh et al. Biological psychiatry global open science 2023 oct

Abstract

BACKGROUND Posttraumatic stress disorder, a consequence of psychological trauma, is associated with increased inflammation and an elevated risk of developing comorbid inflammatory diseases. However, the mechanistic link between this mental health disorder and inflammation remains elusive. We previously found that S100a8 and S100a9 messenger RNA, genes that encode the protein calprotectin, were significantly upregulated in T lymphocytes and positively correlated with inflammatory gene expression and the mitochondrial redox environment in these cells. Therefore, we hypothesized that genetic deletion of calprotectin would attenuate the inflammatory and redox phenotype displayed after psychological trauma. METHODS We used a preclinical mouse model of posttraumatic stress disorder known as repeated social defeat stress (RSDS) combined with pharmacological and genetic manipulation of S100a9 (which functionally eliminates calprotectin). A total of 186 animals (93 control, 93 RSDS) were used in these studies. RESULTS Unexpectedly, we observed worsening of behavioral pathology, inflammation, and the mitochondrial redox environment in mice after RSDS compared with wild-type animals. Furthermore, loss of calprotectin significantly enhanced the metabolic demand on T lymphocytes, suggesting that this protein may play an undescribed role in mitochondrial regulation. This was further supported by single-cell RNA sequencing analysis demonstrating that RSDS and loss of S100a9 primarily altered genes associated with mitochondrial function and oxidative phosphorylation. CONCLUSIONS These data demonstrate that the loss of calprotectin potentiates the RSDS-induced phenotype, which suggests that its observed upregulation after psychological trauma may provide previously unexplored protective functions.
View publication
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更多信息

更多信息
种属 Mouse
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyPlate™ EasySep™ Magnet (Catalog 18102) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
样本来源 Other, Spleen
Selection Method Negative
标记抗体
  1. 抗鼠CD8a抗体，克隆53-6.7
    抗鼠CD8a抗体，克隆53-6.7

    抗小鼠、蟾蜍CD8a的大鼠单克隆IgG2a抗体

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