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StemSpan™-ACF红系扩增培养基

用于培养和扩增人红系细胞的无动物成分培养基

产品号 #(选择产品)

产品号 #09860_C

用于培养和扩增人红系细胞的无动物成分培养基

产品优势

  • 专为红系祖细胞扩增和分化设计的无动物成分培养条件
  • 为搭配使用StemSpan™红系扩增添加物而优化
  • 可从每个起始CD34+细胞产生数千个CD71+GlyA+红系细胞

产品组分包括

  • 专为红系祖细胞扩增和分化设计的无动物成分培养条件
  • 为搭配使用StemSpan™红系扩增添加物而优化
  • 可从每个起始CD34+细胞产生数千个CD71+GlyA+红系细胞

总览

StemSpan™-ACF红系扩增培养基(ACF-E)是一种无动物成分(ACF)培养基,用于人造血细胞的体外培养和扩增。当搭配使用StemSpan™红系扩增添加物(100X; 产品号 #02692)时,该培养基可在ACF培养条件下支持人造血祖细胞扩增并分化为CD71+和血型糖蛋白A+(GlyA+)的成红细胞。该培养基仅包含重组蛋白和合成成分,不含血清或其他人或动物来源的成分。

包含
This product contains only recombinant proteins and synthetic components
 
亚型
专用培养基
 
细胞类型
红系细胞,造血干/祖细胞
 
种属

 
应用
细胞培养,分化,扩增
 
品牌
StemSpan
 
研究领域
干细胞生物学,移植研究
 
制剂类别
Animal Component-Free,无血清,Xeno-Free
 

实验数据

Figure 1. Production of Erythroid Cells from Human CB-Derived CD34+ Cells Cultured in StemSpan™ Media Containing StemSpan™ Erythroid Expansion Supplement

(A) Average numbers of erythroid cells generated after culturing purified CD34+ CB cells (n = 5) for 14 days in StemSpan™ SFEM (black bars), SFEM II (grey bars), or StemSpan™-ACF Erythroid Expansion Medium (ACF-E, orange bars) media containing StemSpan™ Erythroid Expansion Supplement (Catalog #02692). Shown are the number of erythroid cells that express CD71 and/or Glycophorin A (GlyA) per input CD34+ cell. (B) The percentages of the different erythroid cell subsets generated in these cultures are shown, including CD71+GlyA+ erythroblasts, immature CD71+GlyA- erythroid progenitor cells and pro-erythroblasts, and CD71-/lowGlyA+ normoblasts. All three media supported the generation of thousands of erythroid cells per CB-derived CD34+ cell plated.

Table 1. Production of Erythroid Cells from Human CB-Derived CD34+ Cells Cultured in StemSpan™ Media Containing StemSpan™ Erythroid Expansion Supplement

Yields and percentages of erythroid cells produced after culturing purified CD34+ CB cells (n = 5) for 14 days in StemSpan™ SFEM, SFEM II or ACF-E media containing Erythroid Expansion Supplement. Erythroid cells were identified by flow cytometry after staining with antibodies against CD71 and GlyA. The % erythroid cells represent the percentage of cells that express CD71 and/or GlyA.

*CI: Confidence Interval.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
09860
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
09860
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。

更多信息

更多信息
种属 Human
Contains This product contains only recombinant proteins and synthetic components
配方类别 Animal Component-Free, Serum-Free, Xeno-Free
质量保证:

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