Nature Research Round Table: Regulations Around Human Induced Pluripotent Stem Cell Registration
Dr. Andreas Kurtz from the Berlin-Brandenburg Center for Regenerative Therapies discusses data associated with human pluripotent stem cells (hPSCs) as well as the benefits of and regulations around data registration for cell line characterization. Since 2010, Dr. Kurtz has managed hPSCreg, a freely accessible global registry for human hPSC lines. This presentation and the following Q&A session were moderated by Dr. Joanne Mountford from the University of Glasgow.
This presentation was part of a Round Table series titled “Challenges in Ensuring hPSC Quality”, hosted in partnership with Nature Research. Global experts gathered at the Springer Nature headquarters in London, UK, to tackle some of the most pertinent issues impacting the use of human pluripotent stem cells (hPSCs), ranging from fundamental biology research to therapeutic applications. Explore the full series here.
Nature Research Round Table: Maintenance of Human Pluripotent Stem Cells In Vitro
Dr. Tenneille Ludwig from the WiCell Research Institute provides information on in vitro culture systems for ES and iPS cell maintenance. The WiCell Research Institute is widely known for their human pluripotent stem cell (hPSC) banking services, with over 1,350 cell lines available to researchers around the globe. This presentation and the following Q&A session were moderated by Dr. Ludovic Vallier from the Wellcome Trust Sanger Institute.
This presentation was part of a Round Table series titled “Challenges in Ensuring hPSC Quality”, hosted in partnership with Nature Research. Global experts gathered at the Springer Nature headquarters in London, UK, to tackle some of the most pertinent issues impacting the use of human pluripotent stem cells (hPSCs), ranging from fundamental biology research to therapeutic applications. Explore the full series here.
Nature Research Round Table: Pluripotency Tests
Dr. Peter Andrews discusses what the term “pluripotency” means in relation to ES and iPS cells, how the term has evolved, and how to assess pluripotent potential by comparing existing assays. Dr. Andrews is co-director of the Centre for Stem Cell Biology at the University of Sheffield. This presentation and the following Q&A session were moderated by Dr. Ludovic Vallier from the Wellcome Trust Sanger Institute.
This presentation was part of a Round Table series titled “Challenges in Ensuring hPSC Quality”, hosted in partnership with Nature Research. Global experts gathered at the Springer Nature headquarters in London, UK, to tackle some of the most pertinent issues impacting the use of human pluripotent stem cells (hPSCs), ranging from fundamental biology research to therapeutic applications. Explore the full series here.
Note: Some original data from this presentation has been omitted to abide by copyright rules.
Optimized Workflows for High-Efficiency Genome Editing in Stem and Primary Cell Types
CRISPR-Cas genome editing in cell culture systems is a powerful technique for disease modeling and the development of cellular therapies. Compared to work with immortalized cell lines, genome editing of stem and primary cells presents unique challenges, including issues related to efficient delivery and expression of CRISPR machinery, clonogenicity, and cytotoxicity. In this webinar, our in-house expert, Dr. Ashley Watson, discusses optimized workflows for CRISPR-Cas9 genome editing in human pluripotent stem cells and primary T cells.
Development, Compatibility, and Applications of mTeSR™ Plus; an Enhanced Medium for the Maintenance of Human Pluripotent Stem Cells (hPSCs)
High quality hPSC maintenance cultures are the foundation for robust and reproducible downstream applications. In this talk, presented at the 2019 ISSCR Annual Meeting in Los Angeles, in-house scientist Dr. Melanie Kardel is joined by Loren Ornelas and Dr. Dhruv Sareen from the Cedars-Sinai Induced Pluripotent Stem Cell (iPSC) Core. This talk features an overview of mTeSR™ Plus, core services, highlights key collaborations, and describes the core facility’s experience with mTeSR™ Plus including cell line transition and quality control.
EasySep™小鼠TIL（CD45）正选试剂盒
Nature Research Round Table: Regulations Around Human Induced Pluripotent Stem Cell Registration Dr. Andreas Kurtz from the Berlin-Brandenburg Center for Regenerative Therapies discusses data associated with human pluripotent stem cells (hPSCs) as well as the benefits of and regulations around data registration for cell line characterization. Since 2010, Dr. Kurtz has managed hPSCreg, a freely accessible global registry for human hPSC lines. This presentation and the following Q&A session were moderated by Dr. Joanne Mountford from the University of Glasgow.
Nature Research Round Table: Maintenance of Human Pluripotent Stem Cells In Vitro Dr. Tenneille Ludwig from the WiCell Research Institute provides information on in vitro culture systems for ES and iPS cell maintenance. The WiCell Research Institute is widely known for their human pluripotent stem cell (hPSC) banking services, with over 1,350 cell lines available to researchers around the globe. This presentation and the following Q&A session were moderated by Dr. Ludovic Vallier from the Wellcome Trust Sanger Institute.
Nature Research Round Table: Pluripotency Tests Dr. Peter Andrews discusses what the term “pluripotency” means in relation to ES and iPS cells, how the term has evolved, and how to assess pluripotent potential by comparing existing assays. Dr. Andrews is co-director of the Centre for Stem Cell Biology at the University of Sheffield. This presentation and the following Q&A session were moderated by Dr. Ludovic Vallier from the Wellcome Trust Sanger Institute.
How to Count hPSC Aggregates to Determine Plating Density for Maintenance and Differentiation Step-by-step instructions for counting human pluripotent stem cell (hPSC) aggregates to determine and adjust the plating densities for hPSC maintenance and differentiation
Optimized Workflows for High-Efficiency Genome Editing in Stem and Primary Cell Types CRISPR-Cas genome editing in cell culture systems is a powerful technique for disease modeling and the development of cellular therapies. Compared to work with immortalized cell lines, genome editing of stem and primary cells presents unique challenges, including issues related to efficient delivery and expression of CRISPR machinery, clonogenicity, and cytotoxicity. In this webinar, our in-house expert, Dr. Ashley Watson, discusses optimized workflows for CRISPR-Cas9 genome editing in human pluripotent stem cells and primary T cells.
Development, Compatibility, and Applications of mTeSR™ Plus; an Enhanced Medium for the Maintenance of Human Pluripotent Stem Cells (hPSCs) High quality hPSC maintenance cultures are the foundation for robust and reproducible downstream applications. In this talk, presented at the 2019 ISSCR Annual Meeting in Los Angeles, in-house scientist Dr. Melanie Kardel is joined by Loren Ornelas and Dr. Dhruv Sareen from the Cedars-Sinai Induced Pluripotent Stem Cell (iPSC) Core. This talk features an overview of mTeSR™ Plus, core services, highlights key collaborations, and describes the core facility’s experience with mTeSR™ Plus including cell line transition and quality control.
