STEMdiff™ 内皮分化试剂盒

高效将人多能干细胞分化为内皮细胞

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产品号 #（选择产品）

产品号 #08005_C

高效将人多能干细胞分化为内皮细胞

产品优势

高效生成 hPSC 衍生的内皮细胞。
无需细胞富集或分选步骤。
与含有 FBS 的培养基相比，hPSC 衍生的内皮细胞扩增效果更优异。

产品组分包括

STEMdiff™ 内皮诱导培养基试剂盒（目录号 #08005）

STEMdiff™ 内皮诱导培养基，100 mL
STEMdiff™ 内皮扩增基础培养基，120 mL
STEMdiff™ 内皮扩张 5X 补充剂，30 mL
无动物成分细胞贴壁底物，1 mL

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要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。

STEMdiff™ Mesoderm Induction Medium
Defined, xeno-free induction medium for early mesodermal differentiation
Heparin Solution
Cell culture supplement
Y-27632 (Dihydrochloride)
RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2
mTeSR™1
cGMP, feeder-free maintenance medium for human ES and iPS cells
Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells

总览

实验数据

产品说明书及文档

应用领域

总览

STEMdiff™ 内皮细胞分化试剂盒（货号 #08005）包含贴壁底物、无动物成分 (ACF) 内皮细胞诱导培养基和内皮细胞扩增培养基。该试剂盒经过优化，可用于将在康宁Matrigel® 基质上以 mTeSR™1（目录号 #85850）、mTeSR™ Plus（目录号 #05825）或 TeSR™-E8™（目录号 #05990）中维持的人多能干细胞（hPSCs）分化为内皮样细胞。该试剂盒设计用于在使用 STEMdiff™ 中胚层诱导培养基（目录号 #05220，可单独购买）进行早期中胚层诱导后立即进行内皮诱导培养。细胞贴壁底物中的 rhCollagen 成分由 CollPlant 公司生产。

本产品仅供研究使用。如有任何临床或商业应用需求，请联系 STEMCELL。

亚型
专用培养基

细胞类型
内皮细胞

种属
人

应用
分化

品牌
STEMdiff

研究领域
血管生成细胞研究，疾病建模，内皮细胞研究

制剂类别
无血清

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实验数据

Figure 1. Schematic Workflow of Endothelial Induction Using the STEMdiff™ Endothelial Kit

In Phase 1, human embryonic stem (ES) or induced pluripotent stem (iPS) cells are cultured in a TeSR™ maintenance medium (mTeSR™ Plus, mTeSR™1, or TeSR™-E8™). On Day 1 (Phase 2) of the protocol, cells are ready for induction into early mesoderm progenitor cells by replacing TeSR™ medium with STEMdiff™ Mesodermal Induction Medium (MIM). By Day 3 (Phase 3), STEMdiff™ Mesoderm Induction Medium is replaced with STEMdiff™ Endothelial Induction Medium to derive endothelial cells. On Day 7, cells are passaged 5 - 6 times onto cultureware pre-coated with Animal Component-Free Cell Attachment Substrate in STEMdiff™ Endothelial Expansion Medium (Phase 4).

Figure 2. A Representative Flow Cytometric Analysis of Endothelial Marker Expression in hPSC-Derived Endothelial Cells

Human pluripotent stem cell (hPSC; H9 cell line)-derived endothelial cells were obtained at Day 7 using STEMdiff™ Endothelial Induction Medium. Greater than 85% of the cells were CD34+ and had high levels of CD31 and CD144 expression. With subsequent passages, the proportion of cells expressing endothelial markers (CD34+, CD31, and CD144) increased up to passage 5.

Figure 3. STEMdiff™ Endothelial Differentiation Kit Generates Functional hPSC-Derived Endothelial Cells

Endothelial cells generated from hPSCs (F016 cell line) using the STEMdiff™ Endothelial Differentiation Kit take up acetylated LDL when plated at 10,000 cells/cm2. Cells are able to form tubular networks in vitro in a tube formation assay when plated at 20,000 cells/well in a 96 well-plate for 24 hrs.

Figure 4. Endothelial Cells Expand Faster in STEMdiff™ Endothelial Expansion Medium Compared to Serum-Containing Medium

STEMdiff™ Endothelial Expansion Medium (A) sustains expansion rate in later passages and leads to (B) superior expansion of hPSC (C1 cell line)-derived endothelial cells when compared to serum-containing medium.

Figure 5. hPSC-Derived Endothelial Cells Generated Using the STEMdiff™ Endothelial Differentiation Kit Express High Levels of ACE2

(A) hPSC (C1 cell line)-derived endothelial cells were generated using the STEMdiff™ Endothelial Differentiation Kit and expanded in STEMdiff™ Endothelial Expansion Medium for 6 passages at 10,000 cells/cm2. (B) The cells were then analyzed for expression of angiotensin-converting enzyme 2 (ACE2). 85% of cells expressed high levels of ACE2.