产品号 #08005_C
高效将人多能干细胞分化为内皮细胞
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高效将人多能干细胞分化为内皮细胞
Defined, xeno-free induction medium for early mesodermal differentiation
Cell culture supplement
RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2
cGMP, feeder-free maintenance medium for human ES and iPS cells
Compatible antibodies for purity assessment of isolated cells
STEMdiff™ 内皮细胞分化试剂盒(货号 #08005)包含贴壁底物、无动物成分 (ACF) 内皮细胞诱导培养基和内皮细胞扩增培养基。该试剂盒经过优化,可用于将在康宁Matrigel® 基质上以 mTeSR™1(目录号 #85850)、mTeSR™ Plus(目录号 #05825)或 TeSR™-E8™(目录号 #05990)中维持的人多能干细胞(hPSCs)分化为内皮样细胞。该试剂盒设计用于在使用 STEMdiff™ 中胚层诱导培养基(目录号 #05220,可单独购买)进行早期中胚层诱导后立即进行内皮诱导培养。细胞贴壁底物中的 rhCollagen 成分由 CollPlant 公司生产。
本产品仅供研究使用。如有任何临床或商业应用需求,请联系 STEMCELL。
本产品仅供研究使用。如有任何临床或商业应用需求,请联系 STEMCELL。
本产品仅供研究使用。如有任何临床或商业应用需求,请联系 STEMCELL。
本产品仅供研究使用。如有任何临床或商业应用需求,请联系 STEMCELL。
亚型
专用培养基
细胞类型
内皮细胞
种属
人
应用
分化
品牌
STEMdiff
研究领域
血管生成细胞研究,疾病建模,内皮细胞研究
制剂类别
无血清
Figure 1. Schematic Workflow of Endothelial Induction Using the STEMdiff™ Endothelial Kit
In Phase 1, human embryonic stem (ES) or induced pluripotent stem (iPS) cells are cultured in a TeSR™ maintenance medium (mTeSR™ Plus, mTeSR™1, or TeSR™-E8™). On Day 1 (Phase 2) of the protocol, cells are ready for induction into early mesoderm progenitor cells by replacing TeSR™ medium with STEMdiff™ Mesodermal Induction Medium (MIM). By Day 3 (Phase 3), STEMdiff™ Mesoderm Induction Medium is replaced with STEMdiff™ Endothelial Induction Medium to derive endothelial cells. On Day 7, cells are passaged 5 - 6 times onto cultureware pre-coated with Animal Component-Free Cell Attachment Substrate in STEMdiff™ Endothelial Expansion Medium (Phase 4).
Figure 2. A Representative Flow Cytometric Analysis of Endothelial Marker Expression in hPSC-Derived Endothelial Cells
Human pluripotent stem cell (hPSC; H9 cell line)-derived endothelial cells were obtained at Day 7 using STEMdiff™ Endothelial Induction Medium. Greater than 85% of the cells were CD34+ and had high levels of CD31 and CD144 expression. With subsequent passages, the proportion of cells expressing endothelial markers (CD34+, CD31, and CD144) increased up to passage 5.
Figure 3. STEMdiff™ Endothelial Differentiation Kit Generates Functional hPSC-Derived Endothelial Cells
Endothelial cells generated from hPSCs (F016 cell line) using the STEMdiff™ Endothelial Differentiation Kit take up acetylated LDL when plated at 10,000 cells/cm2. Cells are able to form tubular networks in vitro in a tube formation assay when plated at 20,000 cells/well in a 96 well-plate for 24 hrs.
Figure 4. Endothelial Cells Expand Faster in STEMdiff™ Endothelial Expansion Medium Compared to Serum-Containing Medium
STEMdiff™ Endothelial Expansion Medium (A) sustains expansion rate in later passages and leads to (B) superior expansion of hPSC (C1 cell line)-derived endothelial cells when compared to serum-containing medium.
Figure 5. hPSC-Derived Endothelial Cells Generated Using the STEMdiff™ Endothelial Differentiation Kit Express High Levels of ACE2
(A) hPSC (C1 cell line)-derived endothelial cells were generated using the STEMdiff™ Endothelial Differentiation Kit and expanded in STEMdiff™ Endothelial Expansion Medium for 6 passages at 10,000 cells/cm2. (B) The cells were then analyzed for expression of angiotensin-converting enzyme 2 (ACE2). 85% of cells expressed high levels of ACE2.
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种属 | Human |
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配方类别 | Serum-Free |
哺乳动物活细胞计数试剂
Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) 含15 mM HEPES缓冲液
透明聚苯乙烯平底、经组织培养处理的带盖多孔细胞培养板
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