STEMdiff™ 肺祖细胞试剂盒

无血清培养基，用于从人胚胎干细胞和诱导多能干细胞高效且可重复地生成肺祖细胞。

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产品号 #（选择产品）

产品号 #100-0230_C

无血清培养基，用于从人胚胎干细胞和诱导多能干细胞高效且可重复地生成肺祖细胞。

产品优势

使用稳健的培养基将 hPSC 分化为肺祖细胞，经过优化，可与mTeSR™1或mTeSR™ Plus共同使用
获得能够向下游近端和远端气道细胞分化的肺祖细胞
通过优化的无血清配方最大限度地提高实验的可重复性
便捷的格式和易于使用的操作方案

产品组分包括

STEMdiff™ 内胚层基础培养基，100 mL
STEMdiff™ 定型内胚层补充剂 MR (100X)，0.35 mL
STEMdiff™ 定型内胚层补充剂 CJ (100X)，1.1 mL
STEMdiff™ 肺基础培养基，180 mL
STEMdiff™ 肺补充剂 (10X)，20 mL
STEMdiff™ 肺祖细胞补充剂 1 (100X)，0.55 mL
STEMdiff™ 肺祖细胞补充剂 2 (100X)，1.45 mL

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总览

STEMdiff™ 肺祖细胞试剂盒是一种无血清培养基系统，可高效且可重复地从人类胚胎干细胞 (hESC) 和诱导性多能干细胞 (hiPSC) 分化为肺祖细胞，分化过程包括三个阶段：1) 定型内胚层，2) 前肠内胚层，以及 3) 肺祖细胞。分化后的细胞将表达 NKX2.1，这是肺祖细胞的关键标志物。所得细胞可按照已发表的肺部疾病和肺部发育研究方案进一步成熟，分化为近端或远端气道细胞。

实验数据

Illustration showing the protocol, media, and three stages (definitive endoderm, anterior foregut endoderm, and lung progenitors) invoved in the differentiation of human PSCs into lung progenitor cells using the STEMdiff™ Lung Progenitor Kit.

Figure 1. Generation of Lung Progenitor Cells from hESCs/hiPSCs with the STEMdiff™ Lung Progenitor Kit

Human embryonic (hESCs) or induced (hiPSCs) pluripotent stem cell cultures progress through a simple three-stage process to generate lung progenitor cells. hESCs or hiPSCs clumps are first seeded in mTeSR™1 or mTeSR™ Plus. On Day 1, differentiation is initiated with Medium DE-1 (STEMdiff™ Endoderm Basal Medium containing Supplement MR and Supplement CJ). Subsequently, on Days 2 and 3, the medium is changed to Medium DE-2 (STEMdiff™ Endoderm Basal Medium containing Supplement CJ) for definitive endoderm patterning. On Day 4, to initiate anterior foregut endoderm patterning, the endoderm monolayer is passaged in Medium LP-1 (STEMdiff™ Lung Basal Medium, Lung Supplement (10X), and Supplement 1) and Y-27632 (Dihydrochloride). Finally, at Day 7, the cells are differentiated into the lung progenitor stage with Medium LP-2 (STEMdiff™ Lung Basal Medium, Lung Supplement (10X), and Supplement 2).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

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Product Information Sheet

Product Name

STEMdiff™ Lung Progenitor Kit

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