产品号 #100-0230_C
无血清培养基,用于从人胚胎干细胞和诱导多能干细胞高效且可重复地生成肺祖细胞。
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无血清培养基,用于从人胚胎干细胞和诱导多能干细胞高效且可重复地生成肺祖细胞。
cGMP, enzyme-free cell dissociation reagent
Dulbecco’s phosphate-buffered saline without calcium and magnesium
RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2
cGMP, feeder-free maintenance medium for human ES and iPS cells
Compatible antibodies for purity assessment of isolated cells
STEMdiff™ 肺祖细胞试剂盒是一种无血清培养基系统,可高效且可重复地从人类胚胎干细胞 (hESC) 和诱导性多能干细胞 (hiPSC) 分化为肺祖细胞,分化过程包括三个阶段:1) 定型内胚层,2) 前肠内胚层,以及 3) 肺祖细胞。分化后的细胞将表达 NKX2.1,这是肺祖细胞的关键标志物。所得细胞可按照已发表的肺部疾病和肺部发育研究方案进一步成熟,分化为近端或远端气道细胞。
亚型
专用培养基
细胞类型
气道细胞,多能干细胞
种属
人
应用
细胞培养,分化,类器官培养
品牌
STEMdiff
研究领域
疾病建模,药物发现和毒理检测,上皮细胞研究,呼吸系统研究,干细胞生物学
制剂类别
无血清
Figure 1. Generation of Lung Progenitor Cells from hESCs/hiPSCs with the STEMdiff™ Lung Progenitor Kit
Human embryonic (hESCs) or induced (hiPSCs) pluripotent stem cell cultures progress through a simple three-stage process to generate lung progenitor cells. hESCs or hiPSCs clumps are first seeded in mTeSR™1 or mTeSR™ Plus. On Day 1, differentiation is initiated with Medium DE-1 (STEMdiff™ Endoderm Basal Medium containing Supplement MR and Supplement CJ). Subsequently, on Days 2 and 3, the medium is changed to Medium DE-2 (STEMdiff™ Endoderm Basal Medium containing Supplement CJ) for definitive endoderm patterning. On Day 4, to initiate anterior foregut endoderm patterning, the endoderm monolayer is passaged in Medium LP-1 (STEMdiff™ Lung Basal Medium, Lung Supplement (10X), and Supplement 1) and Y-27632 (Dihydrochloride). Finally, at Day 7, the cells are differentiated into the lung progenitor stage with Medium LP-2 (STEMdiff™ Lung Basal Medium, Lung Supplement (10X), and Supplement 2).
请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。
本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。
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种属 | Human |
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配方类别 | Serum-Free |
在气液界面培养的人气道上皮细胞的无血清和无bpe培养基
在气液界面培养的人小气道上皮细胞的无血清和无bpe培养基
无血清和无bpe培养基用于气道类器官的高效建立和分化
Defined animal component-free medium for the differentiation of human ES and iPS cells to definitive endoderm
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