Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R

总览

TRA-1-60R 抗体可与 TRA-1-60（足糖萼蛋白，Podocalyxin）发生反应。TRA-1-60 是一种分子量 > 200 kDa 的多能干细胞特异性蛋白，表达于未分化的人类胚胎干细胞 (ES) 和诱导性多能干细胞 (iPS)、胚胎癌 (EC) 细胞、胚胎生殖细胞 (EG) 以及恒河猴 ES 细胞系的表面。在胚胎癌患者血清中检测到了可溶性 TRA-1-60。该表位在细胞分化过程中丢失，含有唾液酸，并与一种名为足糖萼蛋白的大分子量跨膜蛋白结合。尽管唾液酸化，但 TRA-1-60R 抗体识别的表位对神经氨酸酶治疗具有耐药性。

该抗体克隆已经过验证，可用于标记在 TeSR™-E8™（产品号# 05940）、mTeSR™1（产品号# 85850）和 TeSR™2（产品号# 05860）中生长的人类 ES 和 iPS 细胞，以及用于评估使用 EasySep™ 试剂盒分离的细胞的纯度，包括 EasySep™ 人类 ES/iPS 细胞 TRA-1-60 正选试剂盒（产品号# 18166；可能会观察到部分阻断）和 EasySep™ hESC/hiPSC SSEA-4 正选试剂盒（产品号# 18165）。

亚型
一抗

靶抗原
TRA-1-60 (Podocalyxin)

别名
Podocalyxin；TRA-1

活性物种
人，兔，Rhesus

偶联
Alexa Fluor 488，Biotin 或生物素，PE，未偶联的

宿主物种
小鼠

细胞类型
多能干细胞

种属
人，非人灵长类，其它细胞系

应用
细胞分选，流式细胞术，免疫细胞化学，免疫荧光，免疫沉淀，Western印迹

研究领域
干细胞生物学

克隆
TRA-1-60R

基因编号
5420

同种型
IgM，kappa

查看更多显示更少

实验数据

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control, dashed line histogram) labeled with Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R, Alexa Fluor® 488. Labeling of human ES cells with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, Alexa Fluor® 488 is shown (Catalog #60069AD, solid line histogram).
(B) Human ES cells were cultured in mTeSR™1 on BD Matrigel™-coated glass slides, then fixed and stained with Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R, Alexa Fluor® 488. Inset shows cells labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, Alexa Fluor® 488.
(C) Flow cytometry analysis of human iPS cells labeled with Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R, Alexa Fluor® 488 (filled histogram) or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, Alexa Fluor® 488 (open histogram).
(D) Human iPS cells were cultured in mTeSR™1 on BD Matrigel™-coated glass slides, then fixed and stained with Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R, Alexa Fluor® 488. Inset shows cells labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, Alexa Fluor® 488.

Figure 2. Data for Biotin-Conjugated

Flow cytometry analysis of human ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control, dashed line histogram) labeled with Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R, Biotin followed by streptavidin (SAV) APC (filled histogram). Labeling human ES cells with a mouse IgM, kappa biotin isotype control antibody followed by SAV APC is shown (solid line histogram).

Figure 3. Data for PE-Conjugated

(A) Flow cytometry analysis of human embryonic stem (ES) cells (filled histogram) or HT1080 fibrosarcoma cells (negative control, dashed line histogram) labeled with Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R, PE. Labeling of human ES cells with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE is shown (Catalog #60069PE, solid line histogram).
(B) Human ES cells were cultured in mTeSR™1 on BD Matrigel™-coated glass slides, then fixed and stained with Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R, PE. Inset shows cells labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE.
(C) Flow cytometry analysis of human iPS cells labeled with Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R, PE (filled histogram) or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE (open histogram).
(D) Flow cytometry analysis of human ES cells isolated with the EasySep™ Human ES/iPS Cell TRA-1-60 Positive Selection Kit from a mixed population of ES cells and HT1080 fibrosarcoma cells and labeled with Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R, PE. Histograms show labeling of the starting population containing ~5% ES cells (Start) and the isolated cells (Isolated). Labeling with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE is shown in the bottom panel (open histogram).

Figure 4. Data for Unconjugated

(A) Flow cytometry analysis of human ES cells (filled histogram) or HT1080 fibrosarcoma cells (negative control, dashed line histogram) labeled with Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R, followed by goat anti-mouse IgG, FITC. Labeling of human ES cells with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30 (Catalog #60069) followed by goat anti-mouse IgG, FITC is shown (solid line histogram).
(B) Human ES cells were cultured in mTeSR™1 on BD Matrigel™-coated glass slides, then fixed and stained with Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, followed by goat anti-mouse IgG, FITC.
(C) Flow cytometry analysis of human iPS cells labeled with Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R, followed by goat anti-mouse IgG, FITC (filled histogram) or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, followed by goat anti-mouse IgG, FITC (open histogram).
(D) Western blot analysis of denatured/reduced cell lysates from human ES cells (lane 1) or HT1080 fibrosarcoma cells (negative control, lane 2) with Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R.