抗人 TRA-1-81 抗体，克隆 TRA-1-81

抗人、大鼠、恒河猴TRA181的小鼠单克隆IgM抗体

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产品号 #（选择产品）

产品号 #60065_C

抗人、大鼠、恒河猴TRA181的小鼠单克隆IgM抗体

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总览

实验数据

产品说明书及文档

应用领域

总览

该单克隆抗体可与TRA-1-81上的碳水化合物表位发生反应。TRA-1-81是一种高分子量（> 200 kDa）糖蛋白，表达于人类胚胎癌（EC）、胚胎生殖细胞（EG）、胚胎干细胞（ES）和诱导多能干细胞（iPS），以及恒河猴和大鼠ES细胞系的表面。在小鼠EC、EG、ES或iPS细胞中未观察到免疫反应。TRA-1-81抗体的表位与一种名为足糖萼蛋白（Podocalyxin）的硫酸化角质蛋白跨膜蛋白结合，该蛋白对神经氨酸酶治疗具有抗性。

亚型
一抗

靶抗原
TRA-1-81

别名
Podocalyxin

活性物种
人，大鼠，Rhesus

偶联
Alexa Fluor 488，APC，PE，未偶联的

宿主物种
小鼠

细胞类型
多能干细胞

种属
人，非人灵长类，大鼠

应用
ELISAs，流式细胞术，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹

研究领域
干细胞生物学

克隆
TRA-1-81

基因编号
5420

同种型
IgM，kappa

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实验数据

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of human ES cells labeled with Anti-Human TRA-1-81 Antibody, Clone TRA-1-81, Alexa Fluor® 488 (filled histogram) or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, Alexa Fluor® 488 (Catalog #60069AD) (solid line histogram).

Figure 2. Data for APC-Conjugated

Flow cytometry analysis of human ES cells labeled with Anti-Human TRA-1-81 Antibody, Clone TRA-1-81, APC (filled histogram) or a mouse IgM, kappa APC isotype control antibody (solid line histogram).

Figure 3. Data for PE-Conjugated

(A) Flow cytometry analysis of human ES cells labeled with Anti-Human TRA-1-81 Antibody, Clone TRA-1-81, PE (filled histogram) or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE (Catalog #60069PE) (solid line histogram). (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and labeled with Anti-Human TRA-1-81 Antibody, Clone TRA-1-81, PE. Inset shows cells labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE. (C) Flow cytometry analysis of human iPS cells labeled with Anti-Human TRA-1-81 Antibody, Clone TRA-1-81, PE (filled histogram) or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE (solid line histogram).

Figure 4. Data for Unconjugated

(A) Flow cytometry analysis of human ES cells labeled with Anti-Human TRA-1-81 Antibody, Clone TRA-1-81, followed by goat anti-mouse IgM, FITC (filled histogram), or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30 (Catalog #60069), followed by goat anti-mouse IgM, FITC (solid line histogram). (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and labeled with Anti-Human TRA-1-81, Clone TRA-1-81, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, followed by goat anti-mouse IgG, FITC. (C) Flow cytometry analysis of human iPS cells labeled with Anti-Human TRA-1-81 Antibody, Clone TRA-1-81, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram). (D) Western blot analysis of denatured/reduced cell lysates from human ES cells (lane 1) or HT1080 fibrosarcoma cells (lane 2) with Anti-Human TRA181 Antibody, Clone TRA-1-81.