搜索结果: 'methocult media formulations for human hematopoietic cells serum containing'

抗人SATB2 (GLSS)抗体，Polyclonal 兔Polyclonal抗体，抗人SATB2
抗人CD184（CXCR4）抗体，clone 12G5 小鼠Monoclonal IgG2a抗体，抗人、恒河猴、食蟹猴CD184（CXCR4）
抗人CD34抗体，clone 581 小鼠Monoclonal IgG1抗体，抗人CD34
抗人CD45RO抗体，clone UCHL1 小鼠Monoclonal IgG2a抗体，抗人、黑猩猩、普通狨猴CD45RO
抗人巢蛋白抗体，clone 10C2 小鼠Monoclonal IgG1抗体，抗人、食蟹猴巢蛋白
抗人p63（deltaN）抗体，clone Poly6190 抗人p63（deltaN）的兔Polyclonal抗体
抗人SSEA-5抗体，clone 8e11 抗人SSEA-5小鼠Monoclonal IgG1抗体
产品手册
MethoCult™ Media for Performing Hematopoietic Colony-Forming Unit (CFU) Assays

产品类型：

品牌：

MethoCult

产品号#：

00217

00217UK

00215

00215GER

00215UK

03534

03630

03134

03231

03234

03334

03434

03444

03436

04436

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04431

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04564

07700

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27500

38068

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03

产品名：

MethoCult™GF M3534

MethoCult™M3630

MethoCult™M3134

MethoCult™M3231

MethoCult™M3234

MethoCult™M3334

MethoCult™GF M3434

MethoCult™GF M3434

MethoCult™SF M3436

MethoCult™ SF H4436

MethoCult™ H4034 Optimum启动试剂盒套装

MethoCult™ H4100

MethoCult™H4230

MethoCult™SF H4236

MethoCult™H4431

MethoCult™H4434经典

MethoCult™H4434经典

MethoCult™ H4434 Classic启动试剂盒套装

MethoCult™H4531

MethoCult™H4535富集无EPO

MethoCult™ H4535 Enriched，不含EPO

MethoCult™ SF H4536

入门套件MethoCult™H4534经典无EPO

含 2% FBS的Iscove's MDM

氯化铵溶液

氯化铵溶液

35 mm培养皿

35 mm培养皿

Corning® 60 mm 带网格培养皿

16号钝针

16号钝针

3 mL注射器

3 mL注射器
Lu S-J et al. (JUL 2013) Regenerative medicine 8 4 413--424

3D microcarrier system for efficient differentiation of human pluripotent stem cells into hematopoietic cells without feeders and serum [corrected].

BACKGROUND Human embryonic stem cells (hESCs) have been derived and maintained on mouse embryonic fibroblast feeders to keep their undifferentiated status. To realize their clinical potential,a feeder-free and scalable system for large scale production of hESCs and their differentiated derivatives is required. MATERIALS & METHODS hESCs were cultured and passaged on serum/feeder-free 3D microcarriers for five passages. For embryoid body (EB) formation and hemangioblast differentiation,the medium for 3D microcarriers was directly switched to EB medium. RESULTS hESCs on 3D microcarriers maintained pluripotency and formed EBs,which were ten-times more efficient than hESCs cultured under 2D feeder-free conditions (0.11 ± 0.03 EB cells/hESC input 2D vs 1.19 ± 0.32 EB cells/hESC input 3D). After replating,EB cells from 3D culture readily developed into hemangioblasts with the potential to differentiate into hematopoietic and endothelial cells. Furthermore,this 3D system can also be adapted to human induced pluripotent stem cells,which generate functional hemangioblasts with high efficiency. CONCLUSION This 3D serum- and stromal-free microcarrier system is important for future clinical applications,with the potential of developing to a GMP-compatible scalable system. View Publication

产品类型：

产品号#：

04436

05850

05857

05870

05875

85850

85857

85870

85875

产品名：

MethoCult™ SF H4436

mTeSR™1

mTeSR™1
Limó et al. (NOV 1997) Blood 90 9 3316--21

High-titer retroviral vectors containing the enhanced green fluorescent protein gene for efficient expression in hematopoietic cells.

Retroviral vectors constitute the most efficient system to deliver and integrate foreign genes into mammalian cells. We have developed a producer cell line that yields high titers of amphotropic retroviral vectors carrying the enhanced green fluorescent protein (EGFP) gene,a codon humanized,red-shifted variant of the green fluorescent protein (GFP) gene,which can be used as a selectable marker. We have used a hybrid vector that has been shown to efficiently drive gene expression in hematopoietic cells. Virtually all murine and human cell lines and primary human hematopoietic cells tested were transduced with varying efficiency after incubation with vector-containing supernatants. Human CD34(+) cells obtained from cord blood or aphereses products were transduced using a protocol that involves daily addition of vector-containing supernatants for 6 consecutive days. At day 6,up to 16% of the cells expressed EGFP,as assessed by flow cytometry. Sorted EGFP-expressing cells were able to produce fluorescent hematopoietic colonies. EGFP's main advantages are its fast flow cytometry determination and the possibility of cell sorting and simultaneous evaluation of the transduction efficiency along with other phenotypic markers. View Publication

产品类型：

产品号#：

04034

04044

产品名：

MethoCult™H4034 Optimum

MethoCult™H4034 Optimum
Niwa A et al. (JAN 2011) PLoS ONE 6 7 e22261

A novel Serum-Free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors

Elucidating the in vitro differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells is important for understanding both normal and pathological hematopoietic development in vivo. For this purpose,a robust and simple hematopoietic differentiation system that can faithfully trace in vivo hematopoiesis is necessary. In this study,we established a novel serum-free monolayer culture that can trace the in vivo hematopoietic pathway from ES/iPS cells to functional definitive blood cells via mesodermal progenitors. Stepwise tuning of exogenous cytokine cocktails induced the hematopoietic mesodermal progenitors via primitive streak cells. These progenitors were then differentiated into various cell lineages depending on the hematopoietic cytokines present. Moreover,single cell deposition assay revealed that common bipotential hemoangiogenic progenitors were induced in our culture. Our system provides a new,robust,and simple method for investigating the mechanisms of mesodermal and hematopoietic differentiation. View Publication

产品类型：

产品号#：

05850

05857

05870

05875

85850

85857

85870

85875

产品名：

mTeSR™1

mTeSR™1
产品手册
StemSpan™: Defined Media and Supplements for Hematopoietic Cell Expansion

产品类型：

品牌：

StemSpan

产品号#：

02690

02691

02693

02696

02697

02692

09600

09650

09605

09655

09800

09850

09805

09855

09900

09910

09920

产品名：

StemSpan™CC100

StemSpan™CD34+扩增补充（10X）

StemSpan™髓系扩增补品（100X）

StemSpan™巨核细胞扩增添加物 (100X)

StemSpan™CC110

StemSpan™红系扩增添加物 (100X)

StemSpan™ SFEM

StemSpan™ SFEM

StemSpan™ SFEM II

StemSpan™ SFEM II

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搜索结果: 'methocult media formulations for human hematopoietic cells serum containing'

3D microcarrier system for efficient differentiation of human pluripotent stem cells into hematopoietic cells without feeders and serum [corrected].

High-titer retroviral vectors containing the enhanced green fluorescent protein gene for efficient expression in hematopoietic cells.

A novel Serum-Free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors

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