Quick and Easy Isolation of T Cells and Other Immune Cells from Large-Volume Samples
Large-scale cell isolation is a critical step in cell therapy research. Current methods can be a significant bottleneck in a lab’s workflow, often requiring a full day for sample processing and cell isolation. In this webinar, Dr. Amanda Vanden Hoek and Ms. Vesna Posarac present two novel platforms that enable efficient cell isolation from large-volume samples: the Easy 250 EasySep™ magnet and the RoboSep™-C instrument.
How to Use Primary Cells to Develop the Next Therapy
Human primary cells are cells isolated directly from tissues, including blood and bone marrow. The use of human primary cells increases the physiological relevance of cell culture systems, enabling you to generate meaningful data that is more predictive of in vivo outcomes. Learn about the advantages of starting with human primary cells for your immunology and cell therapy research workflows. Key considerations for choosing the ideal starting material, donor characterization, regulatory challenges, and tips to streamline your cell-based assays with human primary and cultured cells are also covered.
How to Count Cells Using a Hemocytometer
How to prepare and count stained cell samples using a hemocytometer for both total nucleated cell counts and viable cell counts
Development of Simplified and Defined Cell Culture for Human Pluripotent Stem Cells
Human pluripotent stem cells (hPSCs) have the ability to generate all cell types in the human body and can be used in many applications in basic research and translational medicine, including disease modeling, drug screening and cell therapy. Maintenance of high quality hPSCs is dependent on consistent in vitro cell culture conditions and handling techniques. TeSR™-E8™ is a defined, xeno-free medium with a simple and published formula that was developed to meet the demands of a wide range of applications. The E8 culture system has been successfully used to derive and maintain hPSCs, and enzyme-free methods have also been developed for cell expansion and cryopreservation. More recently, defined differentiation conditions have also been established for specific lineages. These developments will provide an efficient and cost-effective E8 platform for the hPSC field. This webinar is presented by Dr. Guokai Chen at the University of Macau.