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  • 搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

搜索结果: 'methocult media formulations for mouse hematopoietic cells serum containing'

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  7. MethoCult™ Media for Performing Hematopoietic Colony-Forming Unit (CFU) Assays
    MethoCult™ Media for Performing Hematopoietic Colony-Forming Unit (CFU) Assays

    产品类型：

    产品号#：

    03534

    03630

    03134

    03231

    03234

    03334

    03434

    03444

    03436

    04436

    04064

    04100

    04230

    04236

    04431

    04434

    04444

    04464

    04531

    04535

    04545

    04536

    04564

    07700

    07800

    07850

    27100

    27150

    27500

    38068

    28110

    28120

    28230

    28240

    产品名：

    MethoCult™GF M3534

    MethoCult™M3630

    MethoCult™M3134

    MethoCult™M3231

    MethoCult™M3234

    MethoCult™M3334

    MethoCult™GF M3434

    MethoCult™GF M3434

    MethoCult™SF M3436

    MethoCult™ SF H4436

    MethoCult™ H4034 Optimum启动试剂盒套装

    MethoCult™ H4100

    MethoCult™H4230

    MethoCult™SF H4236

    MethoCult™H4431

    MethoCult™H4434经典

    MethoCult™H4434经典

    MethoCult™ H4434 Classic启动试剂盒套装

    MethoCult™H4531

    MethoCult™H4535富集无EPO

    MethoCult™ H4535 Enriched，不含EPO

    MethoCult™ SF H4536

    入门套件MethoCult™H4534经典无EPO

    含 2% FBS的Iscove's MDM

    氯化铵溶液

    氯化铵溶液

    35 mm培养皿

    35 mm培养皿

    DNUGridded Scoring Dish, 5/pk

    Corning® 60 mm 带网格培养皿

    16号钝针

    钝针

    3 mL注射器

    3 mL注射器

  9. High-titer retroviral vectors containing the enhanced green fluorescent protein gene for efficient expression in hematopoietic cells.
    文献
    Limó et al. (NOV 1997) Blood 90 9 3316--21

    High-titer retroviral vectors containing the enhanced green fluorescent protein gene for efficient expression in hematopoietic cells.

    Retroviral vectors constitute the most efficient system to deliver and integrate foreign genes into mammalian cells. We have developed a producer cell line that yields high titers of amphotropic retroviral vectors carrying the enhanced green fluorescent protein (EGFP) gene,a codon humanized,red-shifted variant of the green fluorescent protein (GFP) gene,which can be used as a selectable marker. We have used a hybrid vector that has been shown to efficiently drive gene expression in hematopoietic cells. Virtually all murine and human cell lines and primary human hematopoietic cells tested were transduced with varying efficiency after incubation with vector-containing supernatants. Human CD34(+) cells obtained from cord blood or aphereses products were transduced using a protocol that involves daily addition of vector-containing supernatants for 6 consecutive days. At day 6,up to 16% of the cells expressed EGFP,as assessed by flow cytometry. Sorted EGFP-expressing cells were able to produce fluorescent hematopoietic colonies. EGFP's main advantages are its fast flow cytometry determination and the possibility of cell sorting and simultaneous evaluation of the transduction efficiency along with other phenotypic markers. View Publication

    产品类型：

    产品号#：

    04034

    04044

    产品名：

    MethoCult™H4034 Optimum

    MethoCult™H4034 Optimum

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