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EasySep™人记忆CD8+ T细胞富集试剂盒

未标记的人记忆CD8+ T细胞的免疫磁珠负选
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产品号 #（选择产品）

产品号 #19159_C

未标记的人记忆CD8+ T细胞的免疫磁珠负选

产品优势

  • 操作简单、快捷，且无需分离柱
  • 纯度高达92%
  • 获得的活细胞无标记

产品组分包括

  • EasySep™ 人记忆 CD8+ T细胞富集试剂盒（产品号 #19159）
    • EasySep™人记忆 CD8+ T细胞富集抗体混合物，1 mL
    • EasySep™ Magnetic Particles磁珠，3 x 1 mL
  • RoboSep™人记忆 CD8+ T细胞富集试剂盒 （产品号 #19159RF）
    • EasySep™人记忆 CD8+ T细胞富集抗体混合物，1 mL
    • EasySep™ Magnetic Particles磁珠，3 x 1 mL
    • RoboSep™ 缓冲液（产品号 #20104）
    • RoboSep™过滤吸头（产品号 #20125）
专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. EasySep™磁铁
    EasySep™磁铁
  2. EasySep™缓冲液
    EasySep™缓冲液
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

使用EasySep™人记忆CD8+ T细胞富集试剂盒，通过免疫磁珠负选，可轻松高效地从新鲜或冻存的人外周血单个核细胞 (PBMCs)或裂解的白细胞单采术样本中分离出高度纯化且未被标记的人记忆CD8+ T细胞（CD8+CD45RA-CD45RO+）。EasySep™结合了单克隆抗体的特异性和无柱磁性系统的简便性，迄今已广泛应用于发表的研究中超过20年。

在此 EasySep™负选过程中，表达以下标记的非目的细胞通过抗体复合物和磁珠标记的方式被靶向去除：CD4、CD14、CD16、CD19、CD20、CD34、CD36、CD45RA、CD56、CD61、CD66b、CD123、TCRγ/δ和gly A。随后使用EasySep™磁极将磁珠标记的细胞与未标记的目的记忆CD8+ T细胞分离，随后只需将目的细胞倾倒至 新管中即可。磁珠细胞分选获得的记忆CD8+ T细胞即可用于流式细胞术、细胞培养或基于细胞的检测等下游应用。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现全自动化免疫磁珠细胞分选。或选择由EasySep™人记忆CD8+ T细胞富集试剂盒分离的 、符合伦理规范的即用型冻存人原代外周血CD8+记忆T细胞 。探索更多优化您实验流程的产品 ，包括培养基、添加剂、抗体等。

磁极兼容性
• EasySep™磁极（产品号 #18000） • “The Big Easy” EasySep™磁极（产品号 #18001） • RoboSep™-S（产品号 #21000）
 
分类
细胞分选试剂盒
 
细胞类型
T 细胞，T 细胞，CD8+
 
种属
人
 
样本来源
白细胞单采术样本、PBMC
 
分选方法
负选
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫
 

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实验数据

Typical EasySep™ Human Memory CD8+ T Cell Enrichment Profile

Figure 1. Typical EasySep™ Human Memory CD8+ T Cell Enrichment Profile

Starting with mononuclear cells, the memory CD8+ T cell (CD8+CD45RA-CD45RO+) content of the enriched fraction typically ranges from 72 - 92%. In the example above, the purities of the start and enriched fractions are 4.9% and 88.8%, respectively.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human Memory CD8+ T Cell Enrichment Kit
Catalog #
19159
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Human Memory CD8+ T Cell Enrichment Kit
Catalog #
19159RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Memory CD8+ T Cell Enrichment Kit
Catalog #
19159
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Memory CD8+ T Cell Enrichment Kit
Catalog #
19159
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human Memory CD8+ T Cell Enrichment Kit
Catalog #
19159RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human Memory CD8+ T Cell Enrichment Kit
Catalog #
19159RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human Memory CD8+ T Cell Enrichment Kit
Catalog #
19159RF
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (7)

EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Human T Cell Research Product Workflow
Brochure
Human T Cell Research Product Workflow
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep...
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology

常见问题

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

文献 (1)

Human breast tumor-infiltrating CD8+ T cells retain polyfunctionality despite PD-1 expression. C. A. Egelston et al. Nature communications 2018 OCT

Abstract

Functional CD8+ T cells in human tumors play a clear role in clinical prognosis and response to immunotherapeutic interventions. PD-1 expression in T cells involved in chronic infections and tumors such as melanoma often correlates with a state of T-cell exhaustion. Here we interrogate CD8+ tumor-infiltrating lymphocytes (TILs) from human breast and melanoma tumors to explore their functional state. Despite expression of exhaustion hallmarks, such as PD-1 expression, human breast tumor CD8+ TILs retain robust capacity for production of effector cytokines and degranulation capacity. In contrast, melanoma CD8+ TILs display dramatic reduction of cytokine production and degranulation capacity. We show that CD8+ TILs from human breast tumors can potently kill cancer cells via bi-specific antibodies. Our data demonstrate that CD8+ TILs in human breast tumors retain polyfunctionality, despite PD-1 expression, and suggest that they may be harnessed for effective immunotherapies.
View publication
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更多信息

更多信息
种属 Human
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • RoboSep™-S (Catalog #21000)
样本来源 Leukapheresis, PBMC
Selection Method Negative
标记抗体
  1. 抗人CD45RA，克隆HI100
    抗人CD45RA，克隆HI100

    抗人、黑猩猩CD45RA的小鼠单克隆IgG2b抗体

  2. 抗人CD45RO抗体，克隆UCHL1
    抗人CD45RO抗体，克隆UCHL1

    小鼠单克隆IgG2a抗体，抗人、黑猩猩、普通狨猴CD45RO

  3. 抗人CD8a抗体，克隆RPA-T8
    抗人CD8a抗体，克隆RPA-T8

    小鼠单克隆IgG1抗体，抗人、恒河猴、食蟹猴CD8a

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