总览

使用EasySep™人记忆CD8+ T细胞富集试剂盒，通过免疫磁珠负选，可轻松高效地从新鲜或冻存的人外周血单个核细胞 (PBMCs)或裂解的白细胞单采术样本中分离出高度纯化且未被标记的人记忆CD8+ T细胞（CD8+CD45RA-CD45RO+）。EasySep™结合了单克隆抗体的特异性和无柱磁性系统的简便性，迄今已广泛应用于发表的研究中超过20年。

在此 EasySep™负选过程中，表达以下标记的非目的细胞通过抗体复合物和磁珠标记的方式被靶向去除：CD4、CD14、CD16、CD19、CD20、CD34、CD36、CD45RA、CD56、CD61、CD66b、CD123、TCRγ/δ和gly A。随后使用EasySep™磁极将磁珠标记的细胞与未标记的目的记忆CD8+ T细胞分离，随后只需将目的细胞倾倒至新管中即可。磁珠细胞分选获得的记忆CD8+ T细胞即可用于流式细胞术、细胞培养或基于细胞的检测等下游应用。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现全自动化免疫磁珠细胞分选。或选择由EasySep™人记忆CD8+ T细胞富集试剂盒分离的、符合伦理规范的即用型冻存人原代外周血CD8+记忆T细胞。探索更多优化您实验流程的产品，包括培养基、添加剂、抗体等。

在此 EasySep™负选过程中，表达以下标记的非目的细胞通过抗体复合物和磁珠标记的方式被靶向去除：CD4、CD14、CD16、CD19、CD20、CD34、CD36、CD45RA、CD56、CD61、CD66b、CD123、TCRγ/δ和gly A。随后使用EasySep™磁极将磁珠标记的细胞与未标记的目的记忆CD8+ T细胞分离，随后只需将目的细胞倾倒至新管中即可。磁珠细胞分选获得的记忆CD8+ T细胞即可用于流式细胞术、细胞培养或基于细胞的检测等下游应用。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现全自动化免疫磁珠细胞分选。或选择由EasySep™人记忆CD8+ T细胞富集试剂盒分离的、符合伦理规范的即用型冻存人原代外周血CD8+记忆T细胞。探索更多优化您实验流程的产品，包括培养基、添加剂、抗体等。

在此 EasySep™负选过程中，表达以下标记的非目的细胞通过抗体复合物和磁珠标记的方式被靶向去除：CD4、CD14、CD16、CD19、CD20、CD34、CD36、CD45RA、CD56、CD61、CD66b、CD123、TCRγ/δ和gly A。随后使用EasySep™磁极将磁珠标记的细胞与未标记的目的记忆CD8+ T细胞分离，随后只需将目的细胞倾倒至新管中即可。磁珠细胞分选获得的记忆CD8+ T细胞即可用于流式细胞术、细胞培养或基于细胞的检测等下游应用。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现全自动化免疫磁珠细胞分选。或选择由EasySep™人记忆CD8+ T细胞富集试剂盒分离的、符合伦理规范的即用型冻存人原代外周血CD8+记忆T细胞。探索更多优化您实验流程的产品，包括培养基、添加剂、抗体等。

在此 EasySep™负选过程中，表达以下标记的非目的细胞通过抗体复合物和磁珠标记的方式被靶向去除：CD4、CD14、CD16、CD19、CD20、CD34、CD36、CD45RA、CD56、CD61、CD66b、CD123、TCRγ/δ和gly A。随后使用EasySep™磁极将磁珠标记的细胞与未标记的目的记忆CD8+ T细胞分离，随后只需将目的细胞倾倒至新管中即可。磁珠细胞分选获得的记忆CD8+ T细胞即可用于流式细胞术、细胞培养或基于细胞的检测等下游应用。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现全自动化免疫磁珠细胞分选。或选择由EasySep™人记忆CD8+ T细胞富集试剂盒分离的、符合伦理规范的即用型冻存人原代外周血CD8+记忆T细胞。探索更多优化您实验流程的产品，包括培养基、添加剂、抗体等。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • RoboSep™-S (Catalog #21000)

亚型
细胞分选试剂盒

细胞类型
T 细胞，T 细胞，CD8+

种属
人

样本来源
Leukapheresis，PBMC

筛选方法
Negative

应用
细胞分选

品牌
EasySep，RoboSep

研究领域
免疫

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实验数据

Figure 1. Typical EasySep™ Human Memory CD8+ T Cell Enrichment Profile

Starting with mononuclear cells, the memory CD8+ T cell (CD8+CD45RA-CD45RO+) content of the enriched fraction typically ranges from 72 - 92%. In the example above, the purities of the start and enriched fractions are 4.9% and 88.8%, respectively.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Information Sheet

Product Name

EasySep™ Human Memory CD8+ T Cell Enrichment Kit

Catalog #

19159

Lot #

All

Language

English

Document Type

Product Information Sheet

Product Name

RoboSep™ Human Memory CD8+ T Cell Enrichment Kit

Catalog #

19159RF

Lot #

All

Language

English

Document Type

Safety Data Sheet 1

Product Name

EasySep™ Human Memory CD8+ T Cell Enrichment Kit

Catalog #

19159

Lot #

All

Language

English

Document Type

Safety Data Sheet 2

Product Name

EasySep™ Human Memory CD8+ T Cell Enrichment Kit

Catalog #

19159

Lot #

All

Language

English

Document Type

Safety Data Sheet 1

Product Name

RoboSep™ Human Memory CD8+ T Cell Enrichment Kit

Catalog #

19159RF

Lot #

All

Language

English

Document Type

Safety Data Sheet 2

Product Name

RoboSep™ Human Memory CD8+ T Cell Enrichment Kit

Catalog #

19159RF

Lot #

All

Language

English

Document Type

Safety Data Sheet 3

Product Name

RoboSep™ Human Memory CD8+ T Cell Enrichment Kit

Catalog #

19159RF

Lot #

All

Language

English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area

Workflow Stages

Workflow Stages for T Cell Research

Cell Sourcing

Cell Isolation

Cell Activation, Expansion, and Differentiation

Cell Characterization

Workflow Stages for T Cell Engineering

Cell Isolation

Cell Activation, Expansion, and Differentiation

Cell Characterization

Cell Cryopreservation

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x10⁸ cells/mL and a minimum working volume of 100 µL. Samples containing 2x10⁷ cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

EasySep™人记忆CD8+ T细胞富集试剂盒

产品号 #（选择产品）

产品号 #19159_C

产品优势

产品组分包括

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实验数据

产品说明书及文档

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相关材料与文献

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Scientists Helping Scientists™

种属	Human
Magnet Compatibility	• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • RoboSep™-S (Catalog #21000)
样本来源	Leukapheresis, PBMC
Selection Method	Negative

EasySep™人记忆CD8+ T细胞富集试剂盒

产品号 #（选择产品）

产品号 #19159_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

应用领域

相关材料与文献

技术资料 (7)

常见问题

Can EasySep™ be used for either positive or negative selection?

How does the separation work?

Which columns do I use?

How can I analyze the purity of my enriched sample?

Can EasySep™ separations be automated?

Can EasySep™ be used to isolate rare cells?

Are the EasySep™ magnetic particles FACS-compatible?

Can the EasySep™ magnetic particles be removed after enrichment?

Can I alter the separation time in the magnet?

For positive selection, can I perform more than 3 separations to increase purity?

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

文献 (1)

Abstract

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