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EasySep™小鼠CD4+ T细胞分选试剂盒

免疫磁珠负选不带标记的小鼠CD4+ T细胞

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产品号 #（选择产品）

产品号 #19852_C

免疫磁珠负选不带标记的小鼠CD4+ T细胞

产品优势

操作简单、快速
纯度高达96%
无需分离柱
获得不带标记的活细胞

产品组分包括

EasySep™小鼠CD4+ T细胞分选试剂盒（产品号 #19852）

EasySep™小鼠CD4+ T细胞分选抗体混合物，5 mL
EasySep™ Streptavidin RapidSpheres™ 50001磁珠，1 mL
EasySep™小鼠FcR阻断剂，2 mL

RoboSep™小鼠CD4+ T细胞分选试剂盒（产品号 #19852RF）

EasySep™小鼠CD4+ T细胞分选抗体混合物，5 mL
EasySep™ Streptavidin RapidSpheres™ 50001磁珠，1 mL
EasySep™小鼠FcR阻断剂，2 mL
RoboSep™ 缓冲液（产品号 #20104）
RoboSep™过滤吸头（产品号 #20125）

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New look, same high quality and support! You may notice that your kit contents and packaging look slightly different from previous orders. We are currently updating the format of select EasySep™ Mouse kits to include a Mouse FcR blocker instead of Normal Rat Serum. With this change, all components will now be shipped in a single package, while providing the same cell isolation performance as before.

要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。

EasyEights™ EasySep™ Magnet
Multiple sample processing magnet for column-free immunomagnetic cell separation
EasySep™ Buffer
Cell separation buffer
Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells

总览

实验数据

产品说明书及文档

应用领域

总览

使用EasySep™小鼠CD4+ T细胞分选试剂盒，通过免疫磁珠负选，可轻松高效地从脾细胞或其他组织的单细胞悬液中分选高纯度的小鼠CD4+ T细胞。EasySep™技术结合单克隆抗体的特异性和无柱磁分选系统的简便性，已在发表的研究中广泛应用超过20年。

在该EasySep™负选流程中，非目的细胞通过抗体复合物与磁珠标记。表达以下特异性标志物的非目的细胞将被靶向去除：CD11b、CD45R、Ter119、CD8a、CD49b、CD19、CD11c、TCRγδ和CD24。使用EasySep™磁极吸附后，通过简单地将目的细胞倾倒或吸取至一个新的试管中，即可将被磁珠标记的细胞与不带标记的目的细胞分离开来。在短至15分钟的磁珠分选后，目的CD4+ T细胞可立即用于流式细胞术、细胞培养、基于细胞的实验等下游应用。

了解更多EasySep™免疫磁珠技术的工作原理，或者如何通过RoboSep™实现全自动化免疫磁珠细胞分选。探索更多为您的实验流程优化的产品，包括培养基、补充剂、抗体等。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)

亚型
细胞分选试剂盒

细胞类型
T 细胞，T 细胞，CD4+

种属
小鼠

样本来源
其它细胞系，Spleen

筛选方法
Negative

应用
细胞分选

品牌
EasySep，RoboSep

研究领域
免疫

查看更多显示更少

实验数据

Figure 1. Typical EasySep™ Mouse CD4+ T Cell Isolation Profile

Starting with mouse splenocytes, the CD4+ T cell content (CD3+CD4+) of the isolated fraction is 95.4 ± 3% (mean ± SD), using the purple EasySep™ magnet.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Information Sheet 1

Product Name

RoboSep™ Mouse CD4+ T Cell Isolation Kit

Catalog #

19852RF

Lot #

1000119985 or lower

Language

English

Document Type

Product Information Sheet 2

Product Name

RoboSep™ Mouse CD4+ T Cell Isolation Kit

Catalog #

19852RF

Lot #

1000119986 or higher

Language

English

Document Type

Product Information Sheet 1

Product Name

EasySep™ Mouse CD4+ T Cell Isolation Kit

Catalog #

19852

Lot #

1000119986 or higher

Language

English

Document Type

Product Information Sheet 2

Product Name

EasySep™ Mouse CD4+ T Cell Isolation Kit

Catalog #

19852

Lot #

1000119985 or lower

Language

English

Document Type

Safety Data Sheet 1

Product Name

RoboSep™ Mouse CD4+ T Cell Isolation Kit

Catalog #

19852RF

Lot #

All

Language

English

Document Type

Safety Data Sheet 2

Product Name

RoboSep™ Mouse CD4+ T Cell Isolation Kit

Catalog #

19852RF

Lot #

All

Language

English

Document Type

Safety Data Sheet 3

Product Name

RoboSep™ Mouse CD4+ T Cell Isolation Kit

Catalog #

19852RF

Lot #

All

Language

English

Document Type

Safety Data Sheet 4

Product Name

RoboSep™ Mouse CD4+ T Cell Isolation Kit

Catalog #

19852RF

Lot #

All

Language

English

Document Type

Safety Data Sheet 5

Product Name

RoboSep™ Mouse CD4+ T Cell Isolation Kit

Catalog #

19852RF

Lot #

All

Language

English

Document Type

Safety Data Sheet 1

Product Name

EasySep™ Mouse CD4+ T Cell Isolation Kit

Catalog #

19852

Lot #

All

Language

English

Document Type

Safety Data Sheet 2

Product Name

EasySep™ Mouse CD4+ T Cell Isolation Kit

Catalog #

19852

Lot #

All

Language

English

Document Type

Safety Data Sheet 3

Product Name

EasySep™ Mouse CD4+ T Cell Isolation Kit

Catalog #

19852

Lot #

All

Language

English

Document Type

Safety Data Sheet 4

Product Name

EasySep™ Mouse CD4+ T Cell Isolation Kit

Catalog #

19852

Lot #

All

Language

English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area

Workflow Stages

Workflow Stages for T Cell Research

Cell Sourcing

Cell Isolation

Cell Activation, Expansion, and Differentiation

Cell Characterization

相关材料与文献

Novel immunomodulatory properties of adenosine analogs promote their antiviral activity against SARS-CoV-2. G. Monticone et al. EMBO reports 2024 aug

Abstract

The COVID-19 pandemic reminded us of the urgent need for new antivirals to control emerging infectious diseases and potential future pandemics. Immunotherapy has revolutionized oncology and could complement the use of antivirals, but its application to infectious diseases remains largely unexplored. Nucleoside analogs are a class of agents widely used as antiviral and anti-neoplastic drugs. Their antiviral activity is generally based on interference with viral nucleic acid replication or transcription. Based on our previous work and computer modeling, we hypothesize that antiviral adenosine analogs, like remdesivir, have previously unrecognized immunomodulatory properties which contribute to their therapeutic activity. In the case of remdesivir, we here show that these properties are due to its metabolite, GS-441524, acting as an Adenosine A2A Receptor antagonist. Our findings support a new rationale for the design of next-generation antiviral agents with dual - immunomodulatory and intrinsic - antiviral properties. These compounds could represent game-changing therapies to control emerging viral diseases and future pandemics.

View publication

S100a9 Protects Against the Effects of Repeated Social Defeat Stress. C. M. Moshfegh et al. Biological psychiatry global open science 2023 oct

Abstract

BACKGROUND Posttraumatic stress disorder, a consequence of psychological trauma, is associated with increased inflammation and an elevated risk of developing comorbid inflammatory diseases. However, the mechanistic link between this mental health disorder and inflammation remains elusive. We previously found that S100a8 and S100a9 messenger RNA, genes that encode the protein calprotectin, were significantly upregulated in T lymphocytes and positively correlated with inflammatory gene expression and the mitochondrial redox environment in these cells. Therefore, we hypothesized that genetic deletion of calprotectin would attenuate the inflammatory and redox phenotype displayed after psychological trauma. METHODS We used a preclinical mouse model of posttraumatic stress disorder known as repeated social defeat stress (RSDS) combined with pharmacological and genetic manipulation of S100a9 (which functionally eliminates calprotectin). A total of 186 animals (93 control, 93 RSDS) were used in these studies. RESULTS Unexpectedly, we observed worsening of behavioral pathology, inflammation, and the mitochondrial redox environment in mice after RSDS compared with wild-type animals. Furthermore, loss of calprotectin significantly enhanced the metabolic demand on T lymphocytes, suggesting that this protein may play an undescribed role in mitochondrial regulation. This was further supported by single-cell RNA sequencing analysis demonstrating that RSDS and loss of S100a9 primarily altered genes associated with mitochondrial function and oxidative phosphorylation. CONCLUSIONS These data demonstrate that the loss of calprotectin potentiates the RSDS-induced phenotype, which suggests that its observed upregulation after psychological trauma may provide previously unexplored protective functions.

View publication

Anti-HLA-A2-CAR Tregs prolong vascularized mouse heterotopic heart allograft survival. J. C. Wagner et al. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2022 sep

Abstract

Alloantigen-specific regulatory T cell (Treg) therapy is a promising approach for suppressing alloimmune responses and minimizing immunosuppression after solid organ transplantation. Chimeric antigen receptor (CAR) targeting donor alloantigens can confer donor reactivity to Tregs. However, CAR Treg therapy has not been evaluated in vascularized transplant or multi-MHC mismatched models. Here, we evaluated the ability of CAR Tregs targeting HLA-A2 (A2-CAR) to prolong the survival of heterotopic heart transplants in mice. After verifying the in vitro activation, proliferation, and enhanced suppressive function of A2-CAR Tregs in the presence of A2-antigen, we analyzed the in vivo function of Tregs in C57BL/6 (B6) mice receiving A2-expressing heart allografts. A2-CAR Treg infusion increased the median survival of grafts from B6.HLA-A2 transgenic donors from 23 to 99 days, whereas median survival with polyclonal Treg infusion was 35 days. In a more stringent model of haplo-mismatched hearts from BALB/cxB6.HLA-A2 F1 donors, A2-CAR Tregs slightly increased median graft survival from 11 to 14 days, which was further extended to >100 days when combined with a 9-day course of rapamycin treatment. These findings demonstrate the efficacy of CAR Tregs, alone or in combination with immunosuppressive agents, toward protecting vascularized grafts in fully immunocompetent recipients.

View publication

View All Publications

EasySep™小鼠CD4+ T细胞分选试剂盒

产品号 #（选择产品）

产品号 #19852_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

应用领域

相关材料与文献

Request Pricing

更多信息

Scientists Helping Scientists™

种属	Mouse
Magnet Compatibility	• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
样本来源	Other, Spleen
Selection Method	Negative

EasySep™小鼠CD4+ T细胞分选试剂盒

产品号 #（选择产品）

产品号 #19852_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

应用领域

相关材料与文献

技术资料 (12)

常见问题

Can EasySep™ Streptavidin RapidSpheres™ be used for either positive or negative selection?

How does the separation work?

Which columns do I use?

How can I analyze the purity of my enriched sample?

Can EasySep™ Streptavidin RapidSphere™ separations be automated?

Are cells isolated using EasySep™ RapidSphere™ products FACS-compatible?

Can I alter the separation time in the magnet?

文献 (39)

Abstract

Abstract

Abstract

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更多信息

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