技术资料

Robinson M et al. (AUG 2016) Stem Cell Reviews and Reports 12 4 476--483

Functionalizing Ascl1 with Novel Intracellular Protein Delivery Technology for Promoting Neuronal Differentiation of Human Induced Pluripotent Stem Cells

Pluripotent stem cells can become any cell type found in the body. Accordingly,one of the major challenges when working with pluripotent stem cells is producing a highly homogenous population of differentiated cells,which can then be used for downstream applications such as cell therapies or drug screening. The transcription factor Ascl1 plays a key role in neural development and previous work has shown that Ascl1 overexpression using viral vectors can reprogram fibroblasts directly into neurons. Here we report on how a recombinant version of the Ascl1 protein functionalized with intracellular protein delivery technology (Ascl1-IPTD) can be used to rapidly differentiate human induced pluripotent stem cells (hiPSCs) into neurons. We first evaluated a range of Ascl1-IPTD concentrations to determine the most effective amount for generating neurons from hiPSCs cultured in serum free media. Next,we looked at the frequency of Ascl1-IPTD supplementation in the media on differentiation and found that one time supplementation is sufficient enough to trigger the neural differentiation process. Ascl1-IPTD was efficiently taken up by the hiPSCs and enabled rapid differentiation into TUJ1-positive and NeuN-positive populations with neuronal morphology after 8 days. After 12 days of culture,hiPSC-derived neurons produced by Ascl1-IPTD treatment exhibited greater neurite length and higher numbers of branch points compared to neurons derived using a standard neural progenitor differentiation protocol. This work validates Ascl1-IPTD as a powerful tool for engineering neural tissue from pluripotent stem cells. View Publication

产品号#：

05832

05833

05838

05872

05873

05940

07174

07180

07183

07190

27147

07191

36254

07930

07931

07940

07955

07956

07959

07954

27845

27945

27840

27865

27940

27965

05835

05839

08581

08582

100-0483

100-0484

100-1061

07952

100-0763

100-0485

100-1077

产品名：

STEMdiff™ 神经花环选择试剂

STEMdiff™神经前体细胞培养基

STEMdiff™神经祖细胞冻存液

Vitronectin XF™

CellAdhere™ 稀释缓冲液

DMEM/F-12 with 15 mM HEPES

CryoStor® CS10

CryoStor® CS10

CryoStor® CS10

CryoStor® CS10

CryoStor® CS10

STEMdiff™ 神经诱导培养基

STEMdiff™ 神经诱导培养基

STEMdiff™SMADi神经诱导试剂盒

STEMdiff™SMADi神经诱导试剂盒，2套

Hausser Scientificᵀᴹ 明线血球计数板

ReLeSR™

CryoStor® CS10

CryoStor® CS10

Vitronectin XF™

温和细胞解离试剂

ReLeSR™
Er JC et al. (FEB 2015) Angewandte Chemie - International Edition 54 8 2442--2446

Neuo: A fluorescent chemical probe for live neuron labeling

To address existing limitations in live neuron imaging,we have developed NeuO,a novel cell-permeable fluorescent probe with an unprecedented ability to label and image live neurons selectively over other cells in the brain. NeuO enables robust live neuron imaging and isolation in vivo and in vitro across species; its versatility and ease of use sets the basis for its development in a myriad of neuronal targeting applications. View Publication

产品号#：

01801

产品名：

NeuroFluor™ NeuO
科学海报
BrainPhys™ Neuronal Medium Supports the Electrical Activities of Neurons Derived from Human Pluripotent Stem Cells and Primary CNS Tissues in Long-Term Cultures

Conference：

SFN 2016,Advances in Drug Discovery 2017,iForum 2017
47:18

线上讲座
Madeline Lancaster on Brain Organoids: Modeling Human Brain Development in a Dish

发布日期: 09/20/2017
25:30

线上讲座
Brains in a Dish: Using Cerebral Organoids to Study Human Brain Development and Disease

发布日期: 07/03/2017
科学海报
STEMdiff™ Cerebral Organoid Kit: A New Tool for the Culture of 3D Brain Organoids Derived from hPSCs

Conference：

ISSCR 2017
产品手册
神经添加物用于培养高一致性、高品质的神经细胞

品牌：

NeuroCult
56:27

线上讲座
The Road to Functional Human Neuronal Circuits in Vitro

发布日期: 05/03/2016
产品手册
BrainPhys™神经元培养基用于提高神经元功能性

品牌：

BrainPhys
科学海报
BrainPhys™ Neuronal Medium: A Medium Optimized to Support the Synaptic Activity of Neurons Derived from Human Pluripotent Stem Cells and Primary CNS Tissues

Conference：

SFN 2015
29:27

线上讲座
Tired of Manual Neural Rosette Isolation? How to Form and Isolate More Efficiently

发布日期: 04/24/2012
1:05:46

线上讲座
Modeling Human Neurological Disease with Induced Pluripotent Stem Cells

发布日期: 04/25/2014