产品号 #02694_C
无血清培养补充,扩大和分化的人单核细胞
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StemSpan™髓系扩增补充II (100X)含有重组人细胞因子的组合,用于选择性地促进人髓系祖细胞的扩增和分化,从人脐带血(CB)样本中分离的CD34+细胞中产生大量CD14+单核细胞。
StemSpan™髓系扩增补充II (100X)用于与以下任何StemSpan™培养基结合使用:
•StemSpan™SFEM(目录#09600)
StemSpan™SFEM II(目录#09605)
StemSpan™-XF(目录#100-0073)
StemSpan™-AOF(目录#100-0130)
当添加到无血清培养基中时,StemSpan™髓系扩增补充II通常促进每个输入CD34+细胞在CD34+人CB细胞启动的14天液体培养中产生数百个CD14+单核细胞。请参阅data选项卡了解更多详细信息。
Contains
• Recombinant human fms-like tyrosine kinase 3 ligand (Flt3L)
• Recombinant human stem cell factor (SCF)
• Recombinant human thrombopoietin (TPO)
• Recombinant human macrophage colony-stimulating factor (M-CSF)
• Recombinant human granulocyte/macrophage colony-stimulating factor (GM-CSF)
• Supplements
Subtype
Supplements
Cell Type
Hematopoietic Stem and Progenitor Cells, Monocytes, Myeloid Cells
Species
Human
Application
Cell Culture, Differentiation, Expansion
Brand
StemSpan
Area of Interest
Stem Cell Biology, Transplantation Research
Formulation Category
Serum-Free
Figure 1. Production of CD14+ Monocytes by the Expansion and Lineage-Specific Differentiation of Human CB CD34+ Cells Cultured in StemSpan™ SFEM II Containing Myeloid Expansion Supplement II
Flow cytometry dot plots for 14-day cultures of human CB CD34+ cells in StemSpan™ SFEM II containing Myeloid Expansion Supplement II. The plots show expression of CD34 and CD45 (A) before and (B) after culture, and (C) expression of CD13 and CD14 on the expanded cells. The frequency of CD34+ cells declined from 87% before culture to less than 1% after 14 days. CD14+ monocytes increased from < 5% on day 0 to 90% by day 14.
请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。
本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。
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种属 | Human |
---|---|
Contains | • Recombinant human fms-like tyrosine kinase 3 ligand (Flt3L) • Recombinant human stem cell factor (SCF) • Recombinant human thrombopoietin (TPO) • Recombinant human macrophage colony-stimulating factor (M-CSF) • Recombinant human granulocyte/macrophage c |
配方类别 | Serum-Free |
人粒细胞扩增和分化的无血清培养补充物
长期培养人体细胞的培养基
用于培养和扩增造血细胞的无血清培养基
人单核细胞向巨噬细胞分化的无血清培养基
人单核细胞向树突状细胞分化的完整试剂盒
抗人、恒河猴、食蟹猴CD14的小鼠单克隆IgG2a抗体
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